Direct electrochemistry of hemoglobin on graphene/Fe3O4 nanocomposite-modified glass carbon electrode and its sensitive detection for hydrogen peroxide

Abstract

Graphene/Fe 3 O 4 nanocomposite was prepared for the immobilization of hemoglobin (Hb) to improve the electron transfer between Hb and glass carbon electrode (GCE). The characterization of nanocomposites was described by transmission electron microscopy, Fourier transform infrared, Raman spectroscopy, and X-ray photoelectron spectroscopy, respectively. The electrochemistry of Hb on the graphene/ Fe 3 O 4 -based GCE was investigated by cyclic voltammetry and amperometric measurement. The modified electrode showed a wide linear range from 0.25 μmol/L to 1.7 mmol/ L with a correlation coefficient of 0.9967. The detection limit of the H 2 O 2 biosensor was estimated at 6.0×10 −6 mol/L at a signal-to-noise ratio of 3.

Adsorption and desorption studies of lysozyme by Fe3O4–polymer nanocomposite via fluorescence spectroscopy

“…The prominent peaks of Fe 3 O 4 -PAAm magnetic hydrogel in ATR-FTIR curve at 1215 cm À1 , 1365 cm À1 and 1735 cm À1 belong to CAOAC, CAOH and C@O bonds, respectively [23][24][25]. These bonds probably be formed from hydroxide groups of NaOH interact with PAAm.…”

Magnetic Fe3O4-Reduced Graphene Oxide Nanocomposites-Based Electrochemical Biosensing

“…Graphite oxide (GO) was prepared from natural graphite by the modified Hummer's method [21,22]. The superparamagnetic Fe 3 O 4 -RGO nanosheets were prepared via solvothermal method with some modification of those reported literatures [23,24].…”

A novel electrochemical sensor based on Fe2O3 nanoparticles/N-doped graphene for electrocatalytic oxidation of L-cysteine

“…However, the employment of capping agents, which could effectively restrain NPs from aggregating, may severely limit the chemical activities of the NPs, hindering their practical use. To the best of our knowledge, anchoring NPs on GR sheets not only block aggregation to retain their high specific surface area but also improve their electrochemical activity [25][26][27]. For example, iron(III) oxide-graphene nanocomposites have been prepared as novel electrode materials for electrochemical applications [26][27][28].…”