AmperometricL-Lactate Biosensors: 1. Lactic Acid Sensing Electrode Containing Lactate Oxidase in a Composite Poly-L-lysine Matrix

Iwuoha, Emmanuel I.; Rock, Armin; Smyth, Malcolm R.

doi:10.1002/(sici)1521-4109(199905)11:5<367::aid-elan367>3.0.co;2-0

Cited by 41 publications

(7 citation statements)

References 45 publications

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“…The estimated electrode sensitivity was 3.1 mA M À1 and the detection limit was estimated from the noise to signal ratio of the SWV responses and found to be 0.65 mM. this chemical sensor is within the range of 10 À2 and 10 À7 M obtainable for enzyme based biosensors [36,37].…”

Section: Application Of Gce/ppynsa As Phenol Sensormentioning

confidence: 81%

Electrochemical Interrogation and Sensor Applications of Nanostructured Polypyrroles

et al. 2006

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The polymerization of pyrrole in b-naphthalene sulfonic acid (NSA) gave nanotubules, nanomicelles or nanosheets of polypyrrole (PPy) depending on the amount of NSA in the polymer and the temperature of the reaction. Scanning electron microscopy (SEM) measurements showed that the diameters of the nanostructured polypyrrole-bnaphthalene sulfonic acid (PPyNSA) composites were 150 -3000 nm for the tubules, 100 -150 nm for the micelles and 20 nm for the sheets. A red shift in the UV-vis absorption spectra of PPy was observed for PPyNSA which indicates the involvement of bulky b-naphthalene sulfonate ion in the polymerization process. The UV-vis also showed the existence of polaron and bi-polaron in the polymer which may be responsible for the improved solubility of PPyNSA compared to PPy. All the characteristic IR bands of polypyrrole were observed in the FTIR spectra of PPyNSA, with slight variation in the absolute values. However, the absence of NÀH stretching at 3400 cm À1 and 1450 cm À1 usually associated with neutral polypyrrole confirms that the polymer is not in the aromatic state but in the excited polaron and bipolaron defect state. Electrochemical analysis of PPyNSA reveals two redox couples: a/a' -partly oxidized polypyrrole-naphthalene sulfonate radical cation/neutral polypyrrole naphthalene sulfonate; b/b' -fully oxidized naphthalene sulfonate radical cation/partly reduced polypyrrole-naphthalene sulfonate radical anion. The corresponding formal potentials measured at 5 mV/s, E8' (5 mV/s) , are 181 mV and 291 mV, respectively. Amperometric phenol sensor constructed with PPyNSA on a glassy carbon electrode (GCE) gave a sensitivity of 3.1 mA M À1 and a dynamic linear range of 0.65 -139.5 mM. The data for the determination of phenol on the GCE/ PPyNSA electrode was consistent with the electrocatalytic Michaelis-Menten model, giving an apparent MichaelisMenten constant (K M ') value of 160 mM.

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Section: Application Of Gce/ppynsa As Phenol Sensormentioning

confidence: 81%

Electrochemical Interrogation and Sensor Applications of Nanostructured Polypyrroles

et al. 2006

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“…The determination of L‐lactate concentration in blood is essential for the diagnosis of a number of clinical disorders such as heart failure, metabolic disorders, respiratory insufficiency and shocks 1. Lactate levels higher than the reference values of 0.44–1.78 mmol dm −3 in venous blood, are a marker of metabolic, respiratory or hemodynamic disturbances 2. In sports medicine, blood lactate levels during exercise are used for estimating the physical conditions of athletes.…”

Section: Introductionmentioning

confidence: 99%

“…Unfortunately, H 2 O 2 oxidation has been reported to require relatively high applied potentials and is therefore suspectible to interferences from oxidizible molecules such as ascorbic acid, acetaminophen and uric acid 11. In order to minimize the contribution of interfering substances to the biosensor response, different strategies have been presented in the literature including (a) using permselective membranes 2, 12 (b) modification of the electrode by catalysts 2, 13; (c) coimmobilization of LOD with horseradish peroxidase (HRP) 14 and (d) using artificial electron transfer mediators such as ferrocene 15, meldola’s blue 16, ferrocenemethanol 10, quinone 17, tetrathiafulvalene 18 and hydroxymethylferrocene 19.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Lactate Biosensor Based on Carbon Paste Electrode Modified with Benzo[c]cinnoline and Multiwalled Carbon Nanotubes

et al. 2015

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Amperometric lactate biosensor based on a carbon paste electrode modified with benzo[c]cinnoline and multiwalled carbon nanotubes is reported. Incorporation of benzo[c]cinnoline acting as a mediator and multiwalled carbon nanotubes providing a conduction pathway to accelerate electron transfer due to their excellent conductivity into carbon paste matrix resulted in a high performance lactate biosensor. The resulting biosensor exhibited a fast response, high selectivity, good repeatability and storage stability. Under the optimal conditions, the enzyme electrode showed the detection limit of 7.0×10−8 M with a linear range of 2.0×10−7 M–1.1×10−4 M. The usefulness of the biosensor was demonstrated in serum samples.

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“…A few CoPC based schemes have also been developed for the analysis of hydrogen peroxide [17,18], oxygen [19], and hydrazine [20,21]. A few types of CoPC based biosensors have been also developed for the sensing of uric acid [22], glucose [23], lactic acid [24], xanthine [25], cholesterol [26], and organo-phosphate [27]. However, there is no report, yet, of an ethylene bisdithiocarbamate pesticide sensor.…”

Section: Introductionmentioning

confidence: 99%

Determination of an Ethylene Bisdithiocarbamate Based Pesticide (Nabam) by Cobalt Phthalocyanine Modified Carbon Ink Electrode

Lin

Wang

2004

Electroanalysis

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A phthalocyanine based sensor, for anodic detection of sodium ethylene bisdithiocarbamate (Nabam) by coating a mixture of cobalt phthalocyanine (CoPC) modified carbon ink on the surface of a glassy carbon electrode, has been described. The modified ink was prepared by mixing three percent of cobalt phthalocyanine into carbon ink and then diluting the mixture with cyclohexanone in w/w ratio of 1/9. A suitable portion (or 1 mL) of ink mixture was then dipcoated on a rotating disk glassy carbon electrode. The modified ink electrode was air-dried for 15 mins before use. In comparison to the bare ink electrode on which the oxidation of Nabam takes place at 300 mV (vs. 3 M Ag/AgCl), the oxidation potential (À 125 mV) of Nabam at the CoPC modified ink decreases significantly. A typical calibration plot of Nabam proportionally increases over the concentration range of 2.5 to 36 mM (R 0.9990). The detection limit is estimated about 28.8 nM (S/N 3) and its response time (between 10% to 90% of steady-state response) is about 5.3 s at the injection of 5 mM Nabam. The sensitivity requirement of JMPR meeting (Joint FAO/WHO Meeting on Pesticide Residues) for ethylene bisdithiocarbamates (EBDCs) is achieved by this proposed scheme.

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AmperometricL-Lactate Biosensors: 1. Lactic Acid Sensing Electrode Containing Lactate Oxidase in a Composite Poly-L-lysine Matrix

Cited by 41 publications

References 45 publications

Electrochemical Interrogation and Sensor Applications of Nanostructured Polypyrroles

Electrochemical Interrogation and Sensor Applications of Nanostructured Polypyrroles

Amperometric Lactate Biosensor Based on Carbon Paste Electrode Modified with Benzo[c]cinnoline and Multiwalled Carbon Nanotubes

Determination of an Ethylene Bisdithiocarbamate Based Pesticide (Nabam) by Cobalt Phthalocyanine Modified Carbon Ink Electrode

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