Amphiphilic shell nanomagnetic adsorbents for selective and highly efficient capture of low-density lipoprotein from hyperlipidaemia serum

Yu, Yameng; Ma, Boya; Jiang, Xinbang; Guo, Chen; Liu, Zhuang; Li, Nan; Chai, Yamin; Wang, Lichun; Du, Yunzheng; Wang, Biao; Li, Wenzhong; Ou, Lailiang

doi:10.1039/d2tb00291d

Cited by 9 publications

(6 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The electropositivity of LDL is attributed to the presence of arginine and lysine clusters at the 25% position of the carboxyl terminus of apoB-100 . In addition, the presence of a large number of UC and lipophilic moieties on apoB-100 endows LDL with fairly strong hydrophobicity so that both hydrophobic and electrostatic domains are present on the surface of LDL. , On the contrary, the major proprotein domain of HDL, apolipoprotein A-I (apoA-I), demonstrated an electrically neutral property due to the equal numbers of positively and negatively charged amino acid residues. , Therefore, the nanoadsorbent was designed to possess both negatively charged moieties and hydrophobic groups to facilitate the binding with LDL and repulsion of DHL during the adsorption process.…”

Section: Resultsmentioning

confidence: 99%

“…39,40 On the contrary, the major proprotein domain of HDL, apolipoprotein A-I (apoA-I), demonstrated an electrically neutral property due to the equal numbers of positively and negatively charged amino acid residues. 41,42 Therefore, the nanoadsorbent was designed to possess both negatively charged moieties and hydrophobic groups to facilitate the binding with LDL and repulsion of DHL during the adsorption process.…”

Section: Adsorption Experimentmentioning

confidence: 99%

See 1 more Smart Citation

Photorenewable Azobenzene Polymer Brush-Modified Nanoadsorbent for Selective Adsorption of LDL in Serum

Guo

Jiang

et al. 2022

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

The elevated concentration of low-density lipoprotein (LDL) is recognized as a leading factor of hyperlipidemia (HLP), and selective adsorption of serum LDL is regarded as a practical therapy. Based on the superior structure–function characteristics of stimuli-responsive materials, a photorenewable nanoadsorbent (SiO2@Azo@Gly) with high selectivity and reusability was developed using azobenzene as the functional ligand. Its principle was certified by the preparation of silicon nanoparticles with atom transfer radical polymerization (ATRP)-initiating groups via a sol–gel reaction and their subsequent grafting of azobenzene polymer brushes by surface-initiated ATRP, followed by modification with glycine. Immobilization of carboxylated azobenzene polymer brushes onto the nanoparticles endowed SiO2@Azo@Gly with high adsorption selectivity and reusability. The advanced nanoadsorbent exhibited excellent LDL adsorption capacity at about 27 mg/g and could be regenerated by illumination with high efficiency (circulations ≥ 5); this was further verified by transmission electron microscopy (TEM) and Fourier-transform infrared (FTIR) analysis. SiO2@Azo@Gly also demonstrated superior adsorption efficiency and selectivity in serum from HLP patients, the respective adsorption capacities of LDL, triglyceride, and total cholesterol were about 15.65, 24.48, and 28.36 mg/g, and the adsorption to high-density lipoprotein (cardioprotective effect) was only about 3.66 mg/g. Green regeneration of the nanoadsorbent could be achieved completely through a simple photoregeneration process, and the recovery rate was still 97.9% after five regeneration experiments.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Adsorption Experimentmentioning

confidence: 99%

Photorenewable Azobenzene Polymer Brush-Modified Nanoadsorbent for Selective Adsorption of LDL in Serum

Guo

Jiang

et al. 2022

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this study, a simple and convenient method of PDIC is employed for the preparation of microspheres, allowing for the production of a large quantity of microspheres within 3 min. , Rosiglitazone (R), a selective peroxisome proliferator-activated receptor gamma agonist, is chosen as a typical drug to inhibit the inflammatory response in AS. − Acrylic acid (AA) and acrylated chondroitin sodium sulfate (CSA) are used to provide negatively charged sites for LDL adsorption due to their abundant carboxyl and sulfonic acid groups, − and the CSA (structure similar to LDL receptors) has been shown to recognize LDL to some extent. ,, Acrylated beta-cyclodextrin (CD) with a hydrophilic outer cavity and hydrophobic inner cavity is utilized as a weak binding site for R and provides a strong hydrophobic site for LDL after R release. , In brief, monomers are dissolved in deionized water to obtain homogeneous aqueous solution, and then, AA-CSA-CD are prepared through the PDIC in a single droplet. The obtained microspheres are placed in a dimethyl sulfoxide solution of R, and after complete binding of the R, the LDL adsorption microspheres AA-CSA-CD-R are obtained.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Rosiglitazone Release and Low-Density Lipoprotein Removal by Chondroitin Sodium Sulfate/Cyclodextrin/Poly(acrylic acid) Composite Adsorbents for Atherosclerosis Therapy

Xiao,

Xu,

Liu

et al. 2024

Biomacromolecules

View full text Add to dashboard Cite

Atherosclerosis (AS) is characterized by the accumulation of substantial low-density lipoprotein (LDL) and inflammatory response. Hemoperfusion is commonly employed for the selective removal of LDL from the body. However, conventional hemoperfusion merely focuses on LDL removal and does not address the symptom of plaque associated with AS. Based on the LDL binding properties of acrylated chondroitin sodium sulfate (CSA), acrylated beta-cyclodextrin (CD) and acrylic acid (AA), along with the anti-inflammatory property of rosiglitazone (R), the fabricated AA-CSA-CD-R microspheres could simultaneously release R and facilitate LDL removal for hemoperfusion. The AA and CSA offer electrostatic adsorption sites for LDL, while the CD provides hydrophobic adsorption sites for LDL and weak binding sites for R. According to the Sips model, the maximum static LDL adsorption capacity of AA-CSA-CD-R is determined to be 614.73 mg/g. In dynamic simulated perfusion experiments, AA-CSA-CD-R exhibits an initial cycle LDL adsorption capacity of 150.97 mg/g. The study suggests that the weakened inflammatory response favors plaque stabilization. The anti-inflammatory property of the microspheres is verified through an inflammation model, wherein the microsphere extracts are cocultured with mouse macrophages. Both qualitative analysis of iNOS\TNF-α and quantitative analysis of IL-6\TNF-α collectively demonstrate the remarkable anti-inflammatory effect of the microspheres. Therefore, the current study presents a novel blood purification treatment of eliminating pathogenic factors and introducing therapeutic factors to stabilize AS plaque.

show abstract

“…acute coronary syndrome [ 10 ], muscle weakness [ 11 ], muscle pain [ 12 ] and low efficacy) from existing treatments and inefficacy in managing patients with LDL levels above 300 mg/dl [ 13 ] pose significant obstacles to HLP treatment. Studies have indicated that selectively lowering LDL level while maintaining HDL with anti-atherosclerotic properties in patients represents a promising clinical treatment strategy for HLP [ 14 , 15 ]. Encouragingly, hemopurification-based adsorption has shown to be an effective therapy for treating HLP, demonstrating not only good adsorption efficiency for pathogenic LDL and selectivity adsorption resisting HDL, but also a reduction in toxicities, particularly effective for patients with familial hypercholesterolemia [ 16 ] and those who fail to achieve complete recovery through diet and drug therapy.…”

Section: Introductionmentioning

confidence: 99%

Dual stimulus-responsive renewable nanoadsorbent for selective adsorption of low-density lipoprotein in serum

Guo,

Jiang,

Guo

et al. 2024

Regenerative Biomaterials

View full text Add to dashboard Cite

Selective removal of ultra-high low-density lipoprotein (LDL) from the blood of hyperlipemia patients using hemoperfusion is considered an efficient method to prevent the deterioration of atherosclerotic cardiovascular disease. Based on the exceptional structure-function properties of multi-stimulus-responsive materials, we developed a magnetic photorenewable nanoadsorbent (Fe3O4@SiO2@Azo-COOH) with outstanding selectivity and regenerative characteristics, featuring functionalized azobenzene as the ligand. The dual-stimulus response endowed Fe3O4@SiO2@Azo-COOH with rapid separation and photoregenerative properties. The adsorbent demonstrated excellent removal efficiency of LDL with an adsorption capacity of 15.06 mg/g, and highly repetitive adsorption performance (≥5 cycles) under irradiation. Fe3O4@SiO2@Azo-COOH also exhibited remarkable adsorption properties and selectivity in human serum, with adsorption capacities of 10.93, 21.26 and 9.80 mg/g for LDL, total cholesterol and triglycerides, and only 0.77 mg/g for high-density lipoprotein (HDL), resulting in a 93% selective adsorption difference (LDL/HDL). Complete green regeneration of the nanoadsorbent was achieved through a simple regeneration process, maintaining a recovery rate of 99.4% after five regeneration experiments. By combining dynamic perfusion experiment with micromagnetic microfluidics, the LDL content decreased by 16.6%. Due to its superior adsorption capacity and regenerative properties, the dual stimulus-responsive nanosorbent is considered a potential hemoperfusion adsorbent.

show abstract

Amphiphilic shell nanomagnetic adsorbents for selective and highly efficient capture of low-density lipoprotein from hyperlipidaemia serum

Abstract: Removal of low-density lipoprotein (LDL) from hyperlipemia patients’ blood represents an effective approach to prevent the progression of atherosclerotic cardiovascular disease. Based on the LDL structural characteristics and intermolecular interactions,...

Cited by 9 publications

References 51 publications

Photorenewable Azobenzene Polymer Brush-Modified Nanoadsorbent for Selective Adsorption of LDL in Serum

Photorenewable Azobenzene Polymer Brush-Modified Nanoadsorbent for Selective Adsorption of LDL in Serum

Simultaneous Rosiglitazone Release and Low-Density Lipoprotein Removal by Chondroitin Sodium Sulfate/Cyclodextrin/Poly(acrylic acid) Composite Adsorbents for Atherosclerosis Therapy

Dual stimulus-responsive renewable nanoadsorbent for selective adsorption of low-density lipoprotein in serum

Contact Info

Product

Resources

About