Amplicon sequencing reveals significantly increased <i>Vibrio</i> abundance and associated gene functions in vibriosis‐infected black tiger shrimp (<i>Penaeus monodon</i>)

Foysal, Javed; Momtaz, Farhana; Kawser, A. Q. M. Robiul; Ali, Hina; Raihan, Topu; Siddik, Muhammad A.B.; Rahman, Mahbubur; Tay, Chin Yen

doi:10.1111/jfd.13304

Cited by 7 publications

(8 citation statements)

References 47 publications

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“…APM: Vp AHPND ‐infected; CTL: uninfected control; FH: Foysal et al. ( 2021 ) healthy; FI: Foysal et al. ( 2021 ) infected; HH: Hossain et al.…”

Section: Resultsmentioning

confidence: 99%

“…( 2021 ) healthy; FI: Foysal et al. ( 2021 ) infected; HH: Hossain et al. ( 2021 ) healthy; HI: Hossain et al.…”

Section: Resultsmentioning

confidence: 99%

“…The beta diversity parameters, including Bray‐Curtis dissimilarity, Euclidean distance, Jaccard coefficient, and Manhattan distance were also calculated and plotted as PCOA diagrams using Usearch software (Edgar, 2010 ) and R software (Team RC, 2013 ). The raw data sequences (healthy and infected samples) of previous studies (Foysal et al., 2021 ; Hossain et al., 2021 ) were retrieved from NCBI database (BioProject ID: PRJNA662111; PRJNA662500) and used in the beta diversity analysis for cross comparison purpose. A Venn diagram was plotted using Mothur software (Schloss et al., 2009 ) and R software (Team RC, 2013 ).…”

Section: Methodsmentioning

confidence: 99%

“…studies (Foysal et al, 2021;Hossain et al, 2021) were retrieved from NCBI database (BioProject ID: PRJNA662111; PRJNA662500) and used in the beta diversity analysis for cross comparison purpose. A Venn diagram was plotted using Mothur software (Schloss et al, 2009) and R software (Team RC, 2013).…”

Section: F I G U R E 1 Overall Pathogenic and Toxin Flow Of Ahpnd Inf...mentioning

confidence: 99%

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Biochemical indexes and gut microbiota testing as diagnostic methods for Penaeus monodon health and physiological changes during AHPND infection with food safety concerns

Soo

Bhassu

2022

Food Science & Nutrition

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Severe shrimp disease outbreaks have a destructive impact on shrimp aquaculture and its associated downstream food processing industries. Thus, it is essential to develop proper methods for shrimp disease control, which emphasizes the importance of food safety. In this study, we performed biochemical tests and gut microbiome analysis using uninfected control and Vp AHPND ‐infected Penaeus monodon samples. Biochemical tests were performed to assess the phenoloxidase (PO) activity, respiratory Burst (RB) activity, nitrite concentration, superoxide dismutase (SOD) activity, total hemocyte count (THC), and total protein concentrations. Overall, upregulations were detected in these biochemical tests, which showed the activation of the immune response in P. monodon during acute hepatopancreatic necrosis disease (AHPND) infection, especially at 6 hpi and 12 hpi. Besides that, shrimp gut samples were collected and pooled ( n = 3), followed by DNA extraction, PCR amplification targeting the V3/V4 16S ribosomal RNA (rRNA) region, next‐generation sequencing (NGS), and bioinformatics analysis. Proteobacteria was the most abundant phylum in both samples. The Rhodobacteraceae family and Maritimibacter genus were proposed to be vital forshrimp health maintenance. Vp AHPND bacterial colonization and secondary Vibrio infections were postulated to have occurred based on the higher abundances of Vibrionaceae family and Vibrio genus in the Vp AHPND ‐infected sample. Firmicutes phylum together with Photobacterium and Aliiroseovarius genera were inferred to be pathogenic or related factors of AHPND infections. In conclusion, physiology (immune response activation) and gut microbiome changes of disease tolerant P. monodon during AHPND infection were identified. Both biochemical tests and 16S rRNA analysis are proposed as a combined strategy for shrimp health diagnosis for ensuring shrimp health maintenance, disease control, and food safety.

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“…APM: Vp AHPND ‐infected; CTL: uninfected control; FH: Foysal et al. ( 2021 ) healthy; FI: Foysal et al. ( 2021 ) infected; HH: Hossain et al.…”

Section: Resultsmentioning

confidence: 99%

“…( 2021 ) healthy; FI: Foysal et al. ( 2021 ) infected; HH: Hossain et al. ( 2021 ) healthy; HI: Hossain et al.…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: F I G U R E 1 Overall Pathogenic and Toxin Flow Of Ahpnd Inf...mentioning

confidence: 99%

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Biochemical indexes and gut microbiota testing as diagnostic methods for Penaeus monodon health and physiological changes during AHPND infection with food safety concerns

Soo

Bhassu

2022

Food Science & Nutrition

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“…Besides Flavobacterium, Aeromonas bacteria were also found in shrimp A2. The existence of the Aeromonas bacteria group in the study of Foysal et al [36] showed that the genus Aeromonas was the most dominant bacterium, especially in the hepatopancreas of shrimp. Bravo and Figueras [32] explained that Aeromonas can be isolated from the aquatic environment and has been considered a group of pathogenic bacteria in humans and animals.…”

Section: Shrimp A2 Shrimp B2mentioning

confidence: 99%

Identification of bacterial communities related to handling in commercial white-leg shrimp (Litopenaeus vannamei) using metagenomic analysis

Abdullah

Pratama

Sauqi

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Post-harvest handling, which includes the use of cold chains and proper hygiene, plays a critical role in maintaining fish quality and food safety. The bacterial community in white-leg shrimp will be affected by differences in the level of hygiene used in traditional and modern markets. This study was aimed to isolate bacterial DNA and identify bacterial communities on the surface of shrimps sold in the commercial market using metagenomic analysis. The research methods covered the isolation of bacterial DNA followed by the metabarcoding analysis of 16S rRNA and bioinformatics analysis. The sequence abundance of bacteria in White-leg shrimp samples were dominated by the genus Myroides, with a relative abundance of 49.18% in traditional markets and 84.89% in modern markets. Bacterial DNA from the skin surface of shrimp from traditional and modern markets has been isolated. The samples of shrimp from traditional markets had a higher relative abundance and diversity of bacteria than samples from modern markets.

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Gut Microbiome Research: A New Avenue for Aquaculture Disease Management

Foysal

Gupta

Reddy

2021

Biotechnological Advances in Aquaculture Health Management

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Amplicon sequencing reveals significantly increased Vibrio abundance and associated gene functions in vibriosis‐infected black tiger shrimp (Penaeus monodon)

Cited by 7 publications

References 47 publications

Biochemical indexes and gut microbiota testing as diagnostic methods for Penaeus monodon health and physiological changes during AHPND infection with food safety concerns

Biochemical indexes and gut microbiota testing as diagnostic methods for Penaeus monodon health and physiological changes during AHPND infection with food safety concerns

Identification of bacterial communities related to handling in commercial white-leg shrimp (Litopenaeus vannamei) using metagenomic analysis

Gut Microbiome Research: A New Avenue for Aquaculture Disease Management

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