Amplification and methylation of an esterase gene associated with insecticide-resistance in greenbugs, Schizaphis graminum (Rondani) (Homoptera: Aphididae)

Ono, Midori; Swanson, Jeffrey J.; Field, Linda M.; Devonshire, A. L.; Siegfried, Blair D.

doi:10.1016/s0965-1748(99)00082-x

Cited by 39 publications

(20 citation statements)

References 23 publications

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“…This conclusion is supported by results of western blotting using the E4 antiserum of M. persicae where increased signal intensity of the 66 kDa band was observed in the resistant strain. The cross-reactivity exhibited between the 66 kDa band from both resistant and susceptible D. v. virgifera with M. persicae E4 antiserum suggests that the resistance associated esterase is not immunologically distinct from the susceptible esterase and the intensity difference is likely the result of the quantitative rather than qualitative modification of group II esterases [26][27][28][29].…”

Section: Discussionmentioning

confidence: 98%

Partial purification and characterization of a methyl-parathion resistance-associated general esterase in Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae)

Zhou

Scharf

Sarath

et al. 2004

Pesticide Biochemistry and Physiology

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Increased hydrolytic metabolism of organophosphate insecticides has been associated with resistance among Nebraska western corn rootworm populations. In this study, resistance-associated esterases were partially purified by differential centrifugation, ion exchange, and hydroxyapatite column chromatography, with a final purification factor of 100-fold and recovery of approximately 10%. Kinetic analysis of the partially purified enzyme indicated that the K m of the group II esterases was identical for the two populations, although V max was consistently threefold higher in the resistant population. A putative esterase, DvvII, was further purified to homogeneity by preparative polyacrylamide gel electrophoresis. DvvII is a monomer with a molecular weight of approximately 66 kDa, although three distinct isoforms with similar pIs were evident based on isoelectric focusing gel electrophoresis. Immunoassays with the Myzus persicae E4 antiserum indicated that group II esterases from D. v. virgifera were cross-reactive and expressed at much higher titers in the resistant population relative to the susceptible counterpart. These results suggest that the resistance is likely associated with overproduction of an esterase isozyme in resistant D. v. virgifera populations.

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Section: Discussionmentioning

confidence: 98%

Partial purification and characterization of a methyl-parathion resistance-associated general esterase in Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae)

Zhou

Scharf

Sarath

et al. 2004

Pesticide Biochemistry and Physiology

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“…Although insecticide resistance has not, to date, been reported in S. avenae, it has evolved in several other aphid species, including most recently in the greenbug, Schizaphis graminum (Rondani) (Homoptera: Aphididae) (Ono et al, 1999). Our last point of consideration concerns the validity of using suction traps to obtain genetically representative samples of aphids over large areas of their preferred host (ie wheat).…”

Section: Discussionmentioning

confidence: 99%

Evidence for gene flow and local clonal selection in field populations of the grain aphid (Sitobion avenae) in Britain revealed using microsatellites

et al. 2004

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Samples of the grain aphid, Sitobion avenae (F.), a major European pest of cereals, were collected in June and July 1997 from fields sown with winter wheat in a rough transect south-west of Rothamsted, UK. These aphids were genotyped at four microsatellite loci known from previous studies to be highly polymorphic. Allelic frequencies were similar between samples collected in the fields and in the 12.2 m high suction trap at Rothamsted, and there were many widespread genotypes (clones), providing evidence that the species is highly migratory. However, field samples were found to display a high level of genotypic heterogeneity ( ¼ variable clonal composition), most probably the result of clonal selection. The suction trap genotypes sample were slightly different from the field samples, indicative of the inclusion of genotypes from plant hosts (cereals and grasses, Poaceae) other than winter wheat and/or genotype-biased emigration from the field. The relevance of these data to modelling of aphid outbreaks is briefly discussed.

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“…The second change involves modification of regulatory DNA sequences outside the protein coding region, which results in increased protein synthesis. Gene amplification has been conclusively documented in the aphids, Myzus persicae (esterases E4 and FE4) (Field et al, 1988;Field and Devonshire, 1998) and Schizaphis graminum (Type I esterases) Ono et al, 1999), mosquitoes, Culex quinquefasciatus, C. pipens, C. tarsalis, and C. tritaeniorhynchus (esterases A and B) (Mouches et al,1986;Karunaratne et al, 1998;Hemingway et al, 1998), and brown planthoppers, Nilaparvata lugens (NI-EST1) (Small and Hemingway, 2000). The combined results from biochemical, kinetic, and inhibition characterizations (Zhou et al, 2004), as well as immunological analyses (this study) imply an over-expression of the resistance-associated esterase in D. v. virgifera.…”

Section: Discussionmentioning

confidence: 99%

Immunological assessment of an insecticide resistance-associated esterase in the Western corn rootworm

Zhou

Scharf

Meinke

et al. 2005

Arch. Insect Biochem. Physiol.

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In previous investigations, we have determined that organophosphate resistance in the western corn rootworm, Diabrotica virgifera virgifera, is at least partially attributed to a group of non-specific carboxylesterases referred to as group II. Antiserum raised against a purified 66-kDa group II esterase is specific for the denatured enzyme. This antiserum reacts similarly with both beetle homogenates from resistant and susceptible populations, although there is much higher signal intensity in immunoblots of resistant relative to susceptible beetles. These results suggest that overproduction of group II esterases is the underlying basis of esterase-mediated resistance in D. v. virgifera by demonstrating that (1) group II esterases are immunologically indistinguishable between the resistant and susceptible populations, and (2) the intensity differences are due to increased group II esterase proteins in the resistant population. The diagnostic potential of immunological-based assays was tested with a traditional diagnostic concentration bioassay and a biochemical-based native PAGE assay. Significant correlations were observed among all three diagnostic assays (regression coefficients ranging from 0.95 to 0.96). These results demonstrate the importance of the 66-kDa protein as a resistance-associated biochemical marker, thus emphasizing the potential for 66-kDa protein-targeted immunoassays in resistance monitoring programs. Arch. Insect Biochem. Physiol. 58:157-165, 2005.

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Amplification and methylation of an esterase gene associated with insecticide-resistance in greenbugs, Schizaphis graminum (Rondani) (Homoptera: Aphididae)

Abstract: "Amplification and methylation of an esterase gene associated with insecticide-resistance in greenbugs, Schizaphis graminum (Rondani) (Homoptera: Aphididae)" (1999).

Cited by 39 publications

References 23 publications

Partial purification and characterization of a methyl-parathion resistance-associated general esterase in Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae)

Partial purification and characterization of a methyl-parathion resistance-associated general esterase in Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae)

Evidence for gene flow and local clonal selection in field populations of the grain aphid (Sitobion avenae) in Britain revealed using microsatellites

Immunological assessment of an insecticide resistance-associated esterase in the Western corn rootworm

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