Amplification-free detection of HBV DNA mediated by CRISPR-Cas12a using surface-enhanced Raman spectroscopy

Du, Yuwan; Ji, Shuaifeng; Dong, Qingyang; Wang, Jiang; Han, Dianpeng; Gao, Zhixian

doi:10.1016/j.aca.2023.340864

Cited by 23 publications

(8 citation statements)

References 30 publications

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“…Early detection of viral nucleic acids can be crucial in disease prevention, especially for DNA viruses like Hepatitis B virus (HBV), where the detection of DNA genomic material can be specifically recognized by reprogramming CRISPR/Cas12a. In combination with novel gold nanoparticles conjugated to 4-ATP via nonspecific ssDNA as a Raman probe, Du et al established a CRISPR-SERS assay targeting HBV DNA . The Raman reporter molecules can amplify the SERS signal without the need for prior amplification.…”

Section: Crispr/cas12-based Biosensors For Disease Diagnosticsmentioning

confidence: 99%

Exploiting the Specificity of CRISPR/Cas System for Nucleic Acids Amplification-Free Disease Diagnostics in the Point-of-Care

Yee,

Ali,

Mohd-Naim

et al. 2024

Chem Bio Eng.

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Rapid and reliable molecular diagnostics employing target nucleic acids and small biomarkers are crucial strategies required for the precise detection of numerous diseases. Although diagnoses based on nucleic acid recognition are some of the most efficient and precise procedures, these tests often require expensive equipment and skilled professionals. Recent advancements in diagnostic innovations, particularly those based on clustered regularly interspaced short palindromic repeats (CRISPR), aim to provide thorough screening at homes, in clinics, and in the field. In comparison to traditional molecular techniques like PCR, CRISPR/Cas-based detection, using the single-stranded nucleic acid trans-cleavage abilities of Cas12 or Cas13, shows significant potential as a molecular diagnostic tool. It offers benefits such as attomolar-level sensitivity, single-base precision, and rapid turnover rates. Both Cas enzymes demonstrate exceptional specificity and sensitivity, holding substantial promise in disease diagnostics and beyond. Consequently, various amplification-free CRISPR/Cas-based detection methods have emerged, aiming to maintain sensitivity despite the absence of pre-amplification. This allows for the detection of non-nucleic acid targets and facilitates integration into point-of-care settings. This Review highlights current advances in amplification-free CRISPR/Cas detection systems in disease diagnostics and investigates their utility in point-of-care settings. Furthermore, the mechanisms of alternative CRISPR-based amplification-free detection of other small molecules, aside from nucleic acids, for disease diagnosis will also be briefly discussed.

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Section: Crispr/cas12-based Biosensors For Disease Diagnosticsmentioning

confidence: 99%

Exploiting the Specificity of CRISPR/Cas System for Nucleic Acids Amplification-Free Disease Diagnostics in the Point-of-Care

Yee,

Ali,

Mohd-Naim

et al. 2024

Chem Bio Eng.

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“…Besides SARS-CoV-2, the Cas system-based SERS biosensors can also be used to detect HPV, 199,200 and HBV. 201 The use of Prussian blue (PB) under alkaline conditions can produce ferricyanide anions (Fe (CN) 6 4− ) and exhibit a unique characteristic Raman peak located in the "biological Ramansilent region". Pan et al developed a Cas12a-driven SERS biosensor for milk authenticity testing.…”

Section: Application Of Crispr/cas System-based Surfaceenhanced Raman...mentioning

confidence: 99%

“…Besides SARS-CoV-2, the Cas system-based SERS biosensors can also be used to detect HPV, 199,200 and HBV. 201…”

Section: Crispr/cas System In Optical Biosensorsmentioning

confidence: 99%

Recent progress on the CRISPR/Cas system in optical biosensors

Li,

Zhai,

Dong

et al. 2024

Anal. Methods

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“…Based on the advantages of short waiting times and low test environment requirements, this platform is a potential nucleic acid test tool for point-of-care testing. Du et al 63 used a similar method for amplification-free detection of hepatitis B virus (HBV) DNA using AuNPs as the SERS substrate. This system could achieve rapid and highly sensitive detection of HBV DNA within 50 min with a wide detection range of 0.1 pM to 1 nM.…”

Section: Raman Reporter Modifies Metal Surface: Sers Signal Turn-offmentioning

confidence: 99%

Current advance of CRISPR/Cas-based SERS technology

Wang

Chang

et al. 2023

Sens. Diagn.

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Amplification-free detection of HBV DNA mediated by CRISPR-Cas12a using surface-enhanced Raman spectroscopy

Cited by 23 publications

References 30 publications

Exploiting the Specificity of CRISPR/Cas System for Nucleic Acids Amplification-Free Disease Diagnostics in the Point-of-Care

Exploiting the Specificity of CRISPR/Cas System for Nucleic Acids Amplification-Free Disease Diagnostics in the Point-of-Care

Recent progress on the CRISPR/Cas system in optical biosensors

Current advance of CRISPR/Cas-based SERS technology

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