Amplified electrochemical immunoassay for 5-methylcytosine using a nanocomposite prepared from graphene oxide, magnetite nanoparticles and β-cyclodextrin

Zhou, Yunlei; Jiang, Wenjing; Wu, Hui; Liu, Fei; Yin, Huanshun; Lü, Nan; Ai, Shiyun

doi:10.1007/s00604-019-3575-6

Cited by 14 publications

(2 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, this dual-signal amplified biosensor showed a low detection limit of 1.665 pM for m6ATP. Phos-tag-based biorecognition events can introduce functional materials into the biosensing system and result in the generation, enhancement or impairment of the PEC signal [50,51]. The biological recognition system itself can change the photocurrent.…”

Section: Electrochemical Methodsmentioning

confidence: 99%

Biosensors with Metal Ion–Phosphate Chelation Interaction for Molecular Recognition

Hao

Dong

et al. 2023

Molecules

View full text Add to dashboard Cite

Biosensors show promising prospects in the assays of various targets due to their advantages of high sensitivity, good selectivity and rapid response. Molecular recognition is a key event of biosensors, which usually involves the interaction of antigen–antibody, aptamer–target, lectin–sugar, boronic acid–diol, metal chelation and DNA hybridization. Metal ions or complexes can specifically recognize phosphate groups in peptides or proteins, obviating the use of biorecognition elements. In this review, we summarized the design and applications of biosensors with metal ion–phosphate chelation interaction for molecular recognition. The sensing techniques include electrochemistry, fluorescence, colorimetry and so on.

show abstract

Section: Electrochemical Methodsmentioning

confidence: 99%

Biosensors with Metal Ion–Phosphate Chelation Interaction for Molecular Recognition

Hao

Dong

et al. 2023

Molecules

View full text Add to dashboard Cite

show abstract

“…Aiming to reduce the oxidation potential of enzyme products and avoid electrode fouling, a wide variety of alternative substrate/product pairs were developed for ALP-based electrochemical immunosensors, including p -aminophenyl phosphate (PAPP)/ p -aminophenol (PAP) [ 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 ], hydroquinone diphosphate(HQDP)/hydroquinone (HQ) [ 54 , 55 , 56 , 57 , 58 ], p -nitrophenylphosphate (PNPP)/ p -nitrophenol (PNP) [ 59 , 60 , 61 , 62 ], α-naphthyl phosphate (NPP)/α-naphthol [ 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 ], 3-indoxyl phosphate (3-IP)/indigo blue [ ...…”

Section: Electrochemical Methodsmentioning

confidence: 99%

Progress in Electrochemical Immunosensors with Alkaline Phosphatase as the Signal Label

Chen,

La,

et al. 2023

Biosensors

View full text Add to dashboard Cite

Electrochemical immunosensors have shown great potential in clinical diagnosis, food safety, environmental protection, and other fields. The feasible and innovative combination of enzyme catalysis and other signal-amplified elements has yielded exciting progress in the development of electrochemical immunosensors. Alkaline phosphatase (ALP) is one of the most popularly used enzyme reporters in bioassays. It has been widely utilized to design electrochemical immunosensors owing to its significant advantages (e.g., high catalytic activity, high turnover number, and excellent substrate specificity). In this work, we summarized the achievements of electrochemical immunosensors with ALP as the signal reporter. We mainly focused on detection principles and signal amplification strategies and briefly discussed the challenges regarding how to further improve the performance of ALP-based immunoassays.

show abstract

Functionalized graphene oxide nanosheets with unique three-in-one properties for efficient and tunable antibacterial applications

Zhu

Chen

et al. 2020

Nano Res.

View full text Add to dashboard Cite

Amplified electrochemical immunoassay for 5-methylcytosine using a nanocomposite prepared from graphene oxide, magnetite nanoparticles and β-cyclodextrin

Cited by 14 publications

References 33 publications

Biosensors with Metal Ion–Phosphate Chelation Interaction for Molecular Recognition

Biosensors with Metal Ion–Phosphate Chelation Interaction for Molecular Recognition

Progress in Electrochemical Immunosensors with Alkaline Phosphatase as the Signal Label

Functionalized graphene oxide nanosheets with unique three-in-one properties for efficient and tunable antibacterial applications

Contact Info

Product

Resources

About