In the current study; firstly, biodiesel was produced by the transesterification method from refined corn oil. After the biodiesel production, effects of the using neat biodiesel (B100) and different biodiesel/diesel fuel blends (BDFBs) on combustion, engine performance, and exhaust emissions were experimentally investigated in a diesel engine. Also, using the heat release rate (HRR) curves, an empirical correlation was developed for estimating the CD for the test engine. Tests were performed for neat diesel fuel (NDF), B100, and five different BDFBs such as (5, 10, 15, 20, 50; v/v) % under full load and at nine engine speeds. At the end of this study, it has been determined that using biodiesel slightly reduces the brake effective power and effective efficiency, but it increases significantly the specific fuel consumption (BSFC). Using biodiesel increases the BSFC by an average of 3-20%. On the other hand, using biodiesel evidently reduces CO and soot emissions, and increases HC emissions and CO2. The selected BDFBs and B100 increase the cylinder pressures in all selected operating conditions. The use of biodiesel has not shown substantial changes in HRR values. Combustion duration values increase up to (10-15) % biodiesel ratios, after these ratios, they start to decrease.