Amplitude-Dependent Stress Fiber Reorientation in Early Response to Cyclic Strain

Takemasa, Tohru; Sugimoto, Kenkichi; Yamashita, Kazuo

doi:10.1006/excr.1996.3428

Cited by 76 publications

(62 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Indeed, Théry et al [2006] found that full cell spreading is merely followed by reinforcement of the membrane tension by SF formation. Thus, while it is believed that when responding to external mechanical stimuli SFs are reorganized where perturbations in strain or tension are minimized [Takemasa et al, 1997[Takemasa et al, , 1998Hayakawa et al, 2001], the present study suggests that even in the absence of external stimuli SFs are likely to appear in cytoplasmic regions of low strain. A low strain linear region intrinsically appears connecting two separate stress-concentrated points (where maturation of adhesions occurs).…”

mentioning

confidence: 73%

The position and size of individual focal adhesions are determined by intracellular stress‐dependent positive regulation

Deguchi¹,

Matsui²,

Iio³

2011

Cytoskeleton

View full text Add to dashboard Cite

It remains unclear how the subcellular positions and sizes of individual focal adhesions (FAs) are determined in stationary cells. The elucidation of spatial regulation mechanisms is important for accurate understanding of the cellular response to mechanical stress. Through a theoretical analysis on previously reported cell behavior, the present study demonstrates a close correlation between the appearances of mechanosensitive elements and intracellular stress reflecting traction stress that the cell exerts on the substrate. The magnitude and distribution of stress were predicted in this analysis by mimicking intrinsic actomyosin contraction independent of extracellular stimuli. Positions of FAs and actin stress fibers corresponded to the local maximum and minimum stress points, respectively, and thus were determined by the global configuration of cell adhesions. Furthermore, their subcellular sizes were in agreement with the predicted stress magnitudes that were dependent on the local mechanical environment. These results suggest that a positive regulation (i.e., force and cell adhesion enhance each other) functions in the organization of individual FAs in nonmigrating cells.

show abstract

mentioning

confidence: 73%

The position and size of individual focal adhesions are determined by intracellular stress‐dependent positive regulation

Deguchi¹,

Matsui²,

Iio³

2011

Cytoskeleton

View full text Add to dashboard Cite

show abstract

“…Slow stretching of the film induces cell orientation that is parallel to the direction of applied stretch (Collinsworth et al, 2000;Eastwood et al, 1998) but, when the stretch rate exceeds ~1 Hz, cells reorient nearly perpendicular to the stress direction (Kurpinski et al, 2006;Smith et al, 1997;Takemasa et al, 1997). Accordingly, Safran and co-workers have proposed for cells a homeostatic stress in terms of a 'force dipole' at the cell-matrix interface, which cells are programmed to reach over time (De et al, 2007).…”

Section: Cell Morphology and Cytoskeletal Forces Are Directed By Extrmentioning

confidence: 99%

Matrix elasticity, cytoskeletal forces and physics of the nucleus: how deeply do cells ‘feel’ outside and in?

Buxboim

Ivanovska

Discher

2010

Journal of Cell Science

366

333

View full text Add to dashboard Cite

SummaryCellular organization within a multicellular organism requires that a cell assess its relative location, taking in multiple cues from its microenvironment. Given that the extracellular matrix (ECM) consists of the most abundant proteins in animals and contributes both structure and elasticity to tissues, ECM probably provides key physical cues to cells. In vivo, in the vicinity of many tissue cell types, fibrous characteristics of the ECM are less discernible than the measurably distinct elasticity that characterizes different tissue microenvironments. As a cell engages matrix and actively probes, it senses the local elastic resistance of the ECM and nearby cells via their deformation, and -similar to the proverbial princess who feels a pea placed many mattresses below -the cell seems to possess feedback and recognition mechanisms that establish how far it can feel. Recent experimental findings and computational modeling of cell and matrix mechanics lend insight into the subcellular range of sensitivity. Continuity of deformation from the matrix into the cell and further into the cytoskeleton-caged and -linked nucleus also supports the existence of mechanisms that direct processes such as gene expression in the differentiation of stem cells. Ultimately, cells feel the difference between stiff or soft and thick or thin surroundings, regardless of whether or not they are of royal descent.

show abstract

“…Stretching cells beyond a certain amount, thereby increasing pre-extension, causes rapid disruption and reorganization of SFs. This is associated with altered cell morphology and orientation, generally perpendicular to the stretching direction [Banes et al, 1990;Takemasa et al, 1997Takemasa et al, , 1998Wang et al, 2000aWang et al, , 2001aHayakawa et al, 2001;Wille et al, 2004]. On the other hand, pre-extension appears to be critical to SF stability.…”

Section: Introductionmentioning

confidence: 98%

Actin stress fiber pre‐extension in human aortic endothelial cells

Feng

Hucker

et al. 2008

Cell Motil. Cytoskeleton

View full text Add to dashboard Cite

Actin stress fibers (SFs) enable cells to sense and respond to mechanical stimuli and affect adhesion, motility and apoptosis. We and others have demonstrated that cultured human aortic endothelial cells (HAECs) are internally stressed so that SFs are pre-extended beyond their unloaded lengths. The present study explores factors affecting SF pre-extension. In HAECs cultured overnight the baseline pre-extension was 1.10 and independent of the amount of cell shortening. Decreasing contractility with 30 mM BDM or 10 microM blebbistatin decreased pre-extension to 1.05 whereas increasing contractility with 2 nM calyculin A increased pre-extension to 1.26. Knockdown of alpha-actinin-1 with an interfering RNA increased pre-extension to 1.28. None of these affected the wavelength of the buckled SFs. Pre-extension was the same in unperturbed cells as in those in which the actin cytoskeleton was disrupted by both chemical and mechanical means and then allowed to reassemble. Finally, disrupting MTs or IFs did not affect pre-extension but increased the wavelength. Taken together, these results suggest that pre-extension of SFs is determined primarily by intrinsic factors, i.e. the level of actin-myosin interaction. This intrinsic control of pre-extension is sufficiently robust that pre-extension is the same even after the actin cytoskeleton has been disrupted and reorganized. Unlike pre-extension, the morphology of the compressed SFs is partially determined by MTs and IFs which appear to support the SFs along their lengths.

show abstract

Amplitude-Dependent Stress Fiber Reorientation in Early Response to Cyclic Strain

Cited by 76 publications

References 11 publications

The position and size of individual focal adhesions are determined by intracellular stress‐dependent positive regulation

The position and size of individual focal adhesions are determined by intracellular stress‐dependent positive regulation

Matrix elasticity, cytoskeletal forces and physics of the nucleus: how deeply do cells ‘feel’ outside and in?

Actin stress fiber pre‐extension in human aortic endothelial cells

Contact Info

Product

Resources

About