Amycolatopsis eburnea sp. nov., an actinomycete associated with arbuscular mycorrhizal fungal spores

Abstract

A novel actinomycete, designated strain GLM-1T, was isolated from arbuscular mycorrhizal fungal spores from Funneliformis mosseae RYA08, collected from Aquilaria crassna Pierre ex Lec. rhizosphere soil in Klaeng, Rayong Province, Thailand. Morphological characteristics of this strain included long chains of rod-like cells and squarish elements. The cell-wall composition of this novel isolate contained meso-diaminopimelic acid. The whole-cell diagnostic sugars were arabinose and galactose. The predominant menaquinone was MK-9(H4). The major fatty acids were iso-C16 : 0 and iso-C15 : 0. Only phosphatidylethanolamine was detected as a polar lipid. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain GLM-1T was closely related to Amycolatopsis rhabdoformis SB026T (99.11 %) with a low DNA–DNA hybridization value of 22.6–34.7 %. Genome sequencing revealed a genome size of 10 Mbp. There were obvious distinctions in the average nucleotide identity values between stain GLM-1T and its closely related strains at around 86–93 % (ANIb) and 89–94 % (ANIm). The digital DNA–DNA hybridization values between strain GLM-1T and type strains of phylogenetically related species were 34–55 %. The G+C content of the genomic DNA was 71.8 mol%. Based on these data, strain GLM-1T is considered to represent a novel species of the genus Amycolatopsis , for which the name Amycolatopsis eburnea sp. nov. is proposed. The type strain is GLM-1T (=TBRC 9315T=NBRC 113658T)

Isolation, Characterization, and Efficacy of Actinobacteria Associated with Arbuscular Mycorrhizal Spores in Promoting Plant Growth of Chili (Capsicum flutescens L.)

“…Finally, AMF spores were rinsed with sterilized distilled water. The final washing water of the AMF spores was used to isolate actinobacteria on the surface of AMF spores on the actinobacteria isolate agar (Difco TM , New Jersey, USA) following the method described in previous studies [19,20]. The isolation plates were then incubated at 30 • C for 1 month.…”

Photobacterium arenosum WH24, Isolated from the Gill of Pacific Oyster Crassostrea gigas from the North Sea of Germany: Co-cultivation and Prediction of Virulence

“…The Invisorb Spin Plant Mini Kit was used to extract genomic DNA following the instruction of the kit manufacturers (Stratec Molecular, Germany). PCR amplification of the 16S rRNA gene was performed according to Primahana et al [ 25 ] with the primer F27 (5′AGAGTTTGATCMTGGCTCAG3′) and 1492R (5′TACGGYTACCTTGTTACGACTT-3′) [ 26 ]. The 16S rRNA gene was sequenced employing an Applied Biosystems 3730XL automated sequencer (ABI).…”

Winogradskyella luteola sp.nov., Erythrobacter ani sp. nov., and Erythrobacter crassostrea sp.nov., isolated from the hemolymph of the Pacific Oyster Crassostrea gigas

“…The Invisorb Spin Plant Mini Kit was used to extract genomic DNA in accordance with the manufacturer's instructions (Stratec Molecular, Germany).PCR amplification of the 16S rRNA gene was applied with the primer F27 (5′-AGA GTT TGATCMTGG CTC AG-3′) and 1492R (5′-TAC GGY TAC CTT GTT ACG ACTT-3′) (Chaiya et al 2019). The 16S rRNA gene was sequenced employing an Applied Biosystems 3730XL automated sequencer (ABI).…”