Winogradskyella luteola sp.nov., Erythrobacter ani sp. nov., and Erythrobacter crassostrea sp.nov., isolated from the hemolymph of the Pacific Oyster Crassostrea gigas

Abstract

Three new bacterial strains, WHY3T, WH131T, and WH158T, were isolated and described from the hemolymph of the Pacific oyster Crassostrea gigas utilizing polyphasic taxonomic techniques. The 16S rRNA gene sequence analysis revealed that strain WHY3T was a member of the genus Winogradskyella, whereas strains WHI31T and WH158T were members of the genus Erythrobacter. According to the polygenomic study the three strains formed individual lineages with strong bootstrap support. The comparison of dDDH-and ANI values, percentage of conserved proteins (POCP), and average amino acid identity (AAl) between the three strains and their relatives established that the three strains represented two separate genera. Menaquinone-6 was reported as the major respiratory quinone in strain WHY3T and Ubiquinone-10 for strains WH131T and WH158T, respectively. The major cellular fatty acids for strain WHY3T were C15:0, anteiso-C15:1 ω7c, iso-C15:0, C16:1ω7c. The major cellular fatty acids for strains WH131T and WH158T were C14:02-OH and t18:1ω12 for WH131T and C17:0, and C18:1ω7c for strain WH158T. Positive Sudan Black B staining Indicated the presence of polyhydroxyalkanoic acid granules for strains WH131T and WH158T but not for strain WHY3T. The DNA G + C contents of strains WHY3T, WH131T and WH158T were 34.4, 59.7 and 56.6%, respectively. Gene clusters predicted some important genes involved in the bioremediation process. Due to the accomplishment of polyphasic taxonomy, we propose three novel species Winogradskyella luteola sp.nov. (type strain WHY3T = DSM 111804T = NCCB 100833T), Erythrobacter ani sp.nov. (WH131T = DSM 112099T = NCCB 100824T) and Erythrobacter crassostrea sp.nov. (WH158T = DSM 112102T = NCCB 100877T).

Isolation and Cultivation of Carotenoid-Producing Strains from Tidal Flat Sediment and Proposal of Croceibacterium aestuarii sp. nov., a Novel Carotenoid-Producing Species in the Family Erythrobacteraceae

“…However, due to the low bootstrap value and limited recovery nodes, it is challenging to establish conclusive relationships between Croceibacterium species using the 16S ribosomal RNA gene-based phylogeny. Hence, it was imperative to construct a more robust phylogenomic tree to elucidate its evolutionary association with Croceibacterium species, as performed in other Erythrobacteraceae genera recently [23,[51][52][53][54][55][56][57][58][59][60][61].…”

Alteromonas gilva sp. nov. and Erythrobacter fulvus sp. nov., isolated from a tidal mudflat

“…A member of the genus Erythrobacter was first reported by Shiba and Simidu in 1982 [10] and the genus description was subsequently emended by Subhash et al in 2013 [11] and Xu et al in 2020 [12]. According to the LPSN database (http://lpsn.dsmz.de/genus/erythrobacter) [2], the genus currently consists of 15 species and three recently isolated three species include Erythrobacter ani, E. aurantius and E. crassostrea [13,14]. Erythrobacter members have largely been isolated from mudflat, seawater or hot springs [15][16][17][18][19][20][21], and consist of Gram-stain-negative, rod-shaped, and reddish-orange-pigmented bacteria with a DNA G+C content ranging from 63.8 to 67.8 mol% [17].…”

Winogradskyella bathintestinalis sp. nov., isolated from the intestine of the deep-sea loosejaw dragonfish, Malacosteus niger

“…Most recently, 16S rRNA gene amplicons belonging to members of the genus Winogradskyella have been detected in human faecal samples, yet no culturable representatives have been isolated [14]. Furthermore, Winogradskyella are of increasing biotechnological interest due to their antibiofilm activity [15, 16], exopolysaccharide production [17], heavy metal resistance [17] and bioremediation potential [18, 19].…”