Amygdalin induces apoptosis in human cervical cancer cell line HeLa cells

Chen, Yu; Ma, Jinshu; Wang, Fang; Hu, Jie; Ai, Chunqing; Wei, Chengguo; Yang, Qing; Fan, Li

doi:10.3109/08923973.2012.738688

Cited by 102 publications

(74 citation statements)

References 30 publications

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“…Moreover, our results showed that the amygdalin is efficiently induced to the inhibition of cell growth at ∼10 mg/ml concentration as reported in the previous studies (Shragg et al 1982;Chen et al 2013;Makarević et al 2014aMakarević et al , 2014b. However, we interestingly found that the normal somatic cells (MCR-5 fibroblasts) show considerable resistance to the cell growth and senescence at 10 mg/ml concentration of amygdalin.…”

Section: Discussionsupporting

confidence: 90%

“…Cell growth and proliferation were dose-dependently induced in UMUC-3, RT112 and TCCSUP bladder cancer cell lines treated with amygdalin, resulting in arrest of cell cycle progression by down-regulation of cdk2 and cyclin A (Makarević et al 2014a), and amygdalin treatment to SNU-C4 human colon cancer cells also displayed anticancer effect via down-regulation of cell cycle-related genes, implying that the amygdalin compound is induced to incomplete DNA synthesis (Park et al 2005). Furthermore, it has been demonstrated that apoptosis is induced by up-regulating caspase-3 activity and pro-apoptotic Bax protein, whereas down-regulating anti-apoptotic protein Bcl-2 in the amygdalin-treated HeLa cells (Chen et al 2013), human DU145 and LNCaP prostate cancer cells (Chang et al 2006). In another study, it has been shown that amygdalin influences the decrease in adhesion and migratory properties of UMUC-3, TCCSUP and RT112 bladder cancer cells by Figure 5.…”

Section: Discussionmentioning

confidence: 91%

“…Amygdalin, sometimes known as laetrile or vitamin B17, is one of the natural cyanogenic diglucoside existing in the fruits and nuts, such as cherries, apricots kernels, apples and almonds (Holzbecher et al 1984;Santos Pimenta et al 2014). It has been previously reported that amygdalin treatment in human cancer cells exerts a potential cytotoxic and anti-cancer effects by inducing apoptotic cell death (Syrigos et al 1998;Chang et al 2006;Chen et al 2013), arrest cell cycle through downregulation of cell cycle-related genes (Park et al 2005;Makarević et al 2014a) and blocking metastasis that can lead to the spread of cancer to other tissues (Makarević et al 2014b). Primarily, cancer has been treated by using conventional therapies; however, amygdalin is also used as an alternative medicine and was found one of the most useful and effective alternative in cancer therapy (Milazzo et al 2007;Sauer et al 2015).…”

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confidence: 99%

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Inhibition of cell growth and down-regulation of telomerase activity by amygdalin in human cancer cell lines

Moon

Kim

Yun

et al. 2015

Animal Cells and Systems

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The purpose of this study was to examine the effect of amygdalin on cell growth and telomerase activity in human cancer and MRC-5 fibroblast cell lines. The level of β-glucosidase activity for releasing cyanide was significantly (P < .05) higher in cancer cell lines (A-549, MDA-MB-231, MCF-7 and U87-MG) than in MRC-5 fibroblasts. Growth rate of cancer cells was apparently inhibited in concentrations above 10 mg/ml amygdalin with senescent-like abnormal morphology. Whereas the effects were absent or marginally detected in MRC-5 fibroblasts. High incidence of β-galactosidase activity was observed in amygdalin-treated cancer cells, compared with that of untreated control while no difference was observed between the control and amygdalin-treated MRC-5 fibroblasts. Furthermore, level of telomerase activity was significantly (P < .05) higher (∼8-13 fold) in cancer cell lines along with high expression of telomerase reverse transcriptase (TERT) and telomerase RNA component (TERC) than in MRC-5 fibroblasts which did not expressed TERT and TERC. However, telomerase activity was significantly (P < .05) down-regulated in amygdalin-treated cancer cells with the decreased expression of TERT and TERC compared with control cancer cells. There were no difference in the telomerase activity between control and amygdalin-treated MRC-5 fibroblasts. Based on these observations, we concluded that amygdalin treatment offers a valuable option for the cancer treatment, causing inhibition of cell growth and down-regulation of telomerase activity in human cancer cell lines by increasing β-glucosidase activity.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 91%

mentioning

confidence: 99%

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Inhibition of cell growth and down-regulation of telomerase activity by amygdalin in human cancer cell lines

Moon

Kim

Yun

et al. 2015

Animal Cells and Systems

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“…Aghadavod [52] yaptığı literatür incelemesinde, amigdalinin damar sertliği ve şeker hastalığının tedavisinde ve kanser oluşumunun önlenmesinde pozitif etkiye sahip olduğu sonucuna ulaşmıştır. Diğer taraftan amigdalinin antikanserojen etki göstermediği, parçalanma ürünlerinden bazılarının böyle bir aktiviteye sahip olduğu belirlenmiştir [59]. Song ve Xu [19] tarafından amigdalinin tümör hücreleri üzerine etkili olduğu ancak etki mekanizması üzerinde yeterli çalışmanın bulunmadığı ifade edilmiştir.…”

Section: Tedavi Edici Etkisiunclassified

Ami̇gdali̇n Ve Özelli̇kleri̇

Çelik¹,

Yildirim²

2017

Ömer Halisdemir Üniversitesi Mühendislik Bilimleri Dergisi

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ÖZDoğada en çok karşılaşılan siyanojenik glikozit olan amigdalin kayısı, badem, kiraz, elma, erik, armut ve şeftali gibi meyvelerin çekirdeklerinde bulunur. Amigdalin, D(-)-mandelonitril-β-D-gentiobiyozit, D(-)-amigdalin, R-amigdalin, laetril ve vitamin B 17 gibi isimlerle de anılmaktadır. Amigdalinin parçalanması enzimatik aktivite, ısıl işlem, mineral asitler ve yüksek askorbik asit konsantrasyonları ile gerçekleşebilir ve parçalanma sonucunda zehirli hidrojen siyanür oluşur. Amigdalin, alyuvar üretiminin kaybı ile karakterize edilen kansızlık, astım, yüksek tansiyon, damar sertliği, şeker hastalığı, migren ve özellikle de kanser tedavisinde kullanılmaktadır. Laboratuvar koşullarında yapılan bazı çalışmalarda tümör gelişiminin engellenmesine dair olumlu sonuçlar elde edilmiş olmasına karşın özellikle hastalığı ilerlemiş kişilerde amigdalinin tümör gelişimini önlediğine ilişkin ikna edici veriler bulunmamaktadır. Bu nedenle amigdalinin anti-kanserojen etkisinin olup olmadığı konusunun biran önce açıklığa kavuşturulması için ayrıntılı bilimsel çalışmalara ihtiyaç duyulmaktadır. Bu derlemede, amigdalinin kimyasal özellikleri, bulunduğu gıdalar, parçalanması, sağlık üzerine etkisi ve analiz yöntemlerine ilişkin bilgiler verilmiştir.Anahtar Kelimeler: Amigdalin, kimyasal özellikleri, parçalanması, sağlık üzerine etkileri AMYGDALIN AND ITS PROPERTIES ABSTRACTAmygdalin is the most common cyanogenic glycoside in nature, and it is found in fruit kernels, such as apricot, almond, cherry, apple, plum, pear and peach. Amygdalin is also called D(-)-mandelonitrile-β-D-gentiobioside, D(-)-amygdalin, R-amygdalin, laetrile and vitamin B 17 . Amygdalin can be degraded by enzymatic activity, heat treatment, mineral acids or high concentrations of ascorbic acid, and after its degradation, toxic hydrogen cyanide is formed. Amygdalin has been used to treat aplastic anaemia, asthma, hypertension, atherosclerosis, diabetes, migraine, and most important of all, cancer. Although tumour-inhibiting effects of amygdalin have been observed from some in vitro studies, there is no substantial evidence showing that amygdalin induces discrete tumour regression in cancer patients, particularly in those with late-stage disease. Therefore, further investigation is necessary to clarify whether amygdalin has an anti-carcinogenic effect as soon as possible. This paper aims to provide current knowledge about amygdalin regarding its chemical properties, presence in foods, degradation, possible health effects, and analysis methods.

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“…This article is available from: http://www.acanceresearch.com/ standards, regardless of all this Americans use it as drug for cancer treatment [11,12].…”

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confidence: 99%

Review on Pharmacological Activity of Amygdalin

Qadir¹,

Fatima²

2017

Arch Can Res

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Amygdalin is obtained from the pebbles of rosaceous fruits, like apricots, almond, cherries, peaches and plums. It is a plant glucoside which is traditionally used as antitumor drug. It produces synergistic effect if it combines with conditional chemotherapy drugs. Amygdalin also used to cure many other diseases like to control asthma, improve immune system, causes apoptosis of human renal fibroblast, inhibit hyperglycemia. Amygdalin is banning to use as antitumor drug by FDA due to lack evidences of cure in case of cancer but in ancient times it is used as antitumor drug by Chinese.

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Amygdalin induces apoptosis in human cervical cancer cell line HeLa cells

Cited by 102 publications

References 30 publications

Inhibition of cell growth and down-regulation of telomerase activity by amygdalin in human cancer cell lines

Inhibition of cell growth and down-regulation of telomerase activity by amygdalin in human cancer cell lines

Ami̇gdali̇n Ve Özelli̇kleri̇

Review on Pharmacological Activity of Amygdalin

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