Amyloid‐β Inspired Short Peptide Amphiphile Facilitates Synthesis of Silver Nanoparticles as Potential Antibacterial Agents

Abstract

An amyloid‐β inspired biocompatible short peptide amphiphile (sPA) molecule was used for controlled and targeted delivery of bioactive silver nanoparticles via transforming sPA nanostructures. Such sPA‐AgNPs hybrid structures can be further used to develop antibacterial materials to combat emerging bacterial resistance. Due to the excellent antibacterial activity of silver, the growth of clinically relevant bacteria was inhibited in the presence of AgNPs‐sPA hybrids. Bacterial tests demonstrated that the high biocompatibility and low cytotoxicity of the designed sPA allow it to work as a model drug delivery agent. It therefore shows great potential in locally addressing bacterial infections. The results of our study suggest that these nanodevices have the potential to trap and then engage in the facile delivery of their chemical payload at the target site, thereby working as potential delivery materials. This system has potential therapeutic value for the treatment of microbiota triggered progression of neurodegenerative diseases.

Antimicrobial Activity of Silver Nanoparticles Loaded Biomimetic Isomeric Short Lipopeptide Nanostructures

“…The high‐resolution TEM image (Figure 8E, inset) of AgNPs showed that d spacing was 2.6 Å along with the selected area electron diffraction (SAED) pattern and indexing of rings as (111), (200), (220), and (311) also confirmed polycrystalline and fcc nature of AgNP (Figure 8F). This is in good agreement with reported literature [24,25] . A such drastic change in morphology of sPA2 nanostructures (Figure 1F and 2 B) in the presence of AgNPs is perhaps due to shuffled key amino acid sequence in sPA2 .…”

“…Therefore, an alternative approach is needed to minimize the toxic effects of AgNPs. Based on available reports and our lab protocols regarding the synthesis of metal nanoparticles, sPA1 and 2 exhibits the potential to synthesize AgNPs by facile method [24–27] . Hence, to prepare AgNPs with help of sPA1 and 2 , 200 μM solution of each sPA with 200 μM of Ag(I) ions were incubated in sunlight for 5–10 minutes [24,28] …”

“…It is documented that the presence of α‐helix or β‐sheet‐type secondary structures plays an important role during cellular uptake, where the presence of helical structures indicates more cell‐penetrating abilities [43] . Therefore, in presence of metal nanoparticles, these sPA molecules can potentially act as efficient drug carrier agents to deliver encapsulated bioactive nanoparticles to targeted sites [44,25] . Cytotoxicity assays against healthy eukaryotic cells were performed in the presence of sPA1 & 2 , and their AgNPs hybrids (Figure 11 and ESI) [40–41] .…”

“…Therefore, we investigated concentration-dependent self-assembly behavior of short peptide amphiphiles (sPA 1 and 2, Scheme 1), followed by their surface modification by using bioactive silver nanoparticles (AgNPs) to develop AgNP functionalized antibacterial biomaterials. [24,25] Such AgNPs-sPA nanostructures have ability to facilitate transportation of AgNPs via cell membrane, therefore can be convenient drug-delivery agents. [25] In this study, we wished to perform self-assembly of sPA1 and 2 at different concentrations [26] , i. e., 10 μM, 100 μM, and 200 μM, followed by the formation of AgNPs.…”

Creating de novo peptide-based bioactivities: from assembly to origami

“…70 This mortise-tenon joint is independent of the properties of the material encapsulated inside the polymer, and with this advantage, nanostructures with a discrete number of ligands (i.e., peptides) can be obtained by gel electrophoresis. 71 Such peptide/protein-nanoparticle 72 hybrid building blocks hold great potential for bottom-up origami-like nanofabrication, with which Parak and co-workers demonstrated the signicant differences of nanoparticles conjugated with a different number of antibodies in tumor-targeting therapy. 73 Articial light-harvesting complexes have been a hot spot to use "innite" light energy due to the depleting fossil fuels such as coal, oil and natural gas.…”