Amyloidogenic Proteins and Peptides

Complementary DNAs encoding human stefins A (HSA) and B (HSB) were synthesized using Pichia-preferred codons by overlap extension PCR. The full-length genes were ligated downstream of the glyceraldehyde-3-phosphate dehydrogenase promoter in the Pichia expression vector pGAPZαC and successfully expressed in Pichia pastoris strain X-33. Functional recombinant HSA and HSB proteins were purified from culture medium at yields of 121.3 ± 13.5 (n = 3) and 95.4 ± 4.1 (n = 3) mg/L, respectively. Using this expression strategy, we demonstrated that high levels of bioactive recombinant HSA and HSB can be produced by fermentation in P. pastoris.

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Functional expression of amyloidogenic human stefins A and B in Pichia pastoris using codon optimization

Nakamura

Maeda

Morimoto

et al. 2013

Biotech and App Biochem

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Amyloidogenic Proteins and Peptides

Cited by 1 publication

References 104 publications

Functional expression of amyloidogenic human stefins A and B in Pichia pastoris using codon optimization

Functional expression of amyloidogenic human stefins A and B in Pichia pastoris using codon optimization

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