An 85-kilodalton surface antigen gene family of Trypanosoma cruzi encodes polypeptides homologous to bacterial neuraminidases

Takle, Garry B.; Cross, George A.M.

doi:10.1016/0166-6851(91)90114-l

Cited by 54 publications

(33 citation statements)

References 49 publications

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“…The gp82 sequence shares a considerable degree of homology with members of the gp85 family expressed in tissue culture or blood trypomastigotes (9,11,13,26). Alignments by BLAST algorithm have revealed 60 to 65% identities between the central domain of metacyclic trypomastigote gp82 and the C-terminal region glycoproteins of the gp85 family and 69% identity with the C-terminal region of an amastigote surface protein.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Cell Adhesion Site ofTrypanosoma cruziMetacyclic Stage Surface Glycoprotein gp82

Manque¹,

Eichinger

Juliano

et al. 2000

Infect Immun

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The surface glycoprotein gp82, expressed in the insect-stage metacyclic trypomastigotes of Trypanosoma cruzi, has been implicated in mammalian cell invasion. Here we have characterized the cell adhesion site of gp82 by using recombinant proteins and synthetic peptides based on gp82. The recombinant protein Del-4/8, lacking 65 amino acids of gp82 central domain (at positions 257 to 321), was virtually devoid of cell-binding activity and lacked the ability to inhibit parasite invasion, in contrast to J18, the construct containing the full-length gp82 sequence (amino acids 1 to 516). Constructs with shorter deletions, i.e., Del-4 (deleted from 257 to 271) and Del-8 (deleted from 293 to 321), bound to target cells to a significantly lesser degree than did J18. The sites deleted in recombinant proteins Del-4 and Del-8 contained acidic amino acids critical for cell adhesion. Thus, the cell-binding capacity of protein Del-E/D, lacking the glutamic acid (259/260) and aspartic acid (303/304) pairs, was negligible, as was its capacity to inhibit parasite internalization. Of a set of synthetic peptides spanning the gp82 central domain, a 22-mer hybrid peptide, p4/8, formed by two noncontiguous sequences (at positions 257 to 273 and 302 to 306) and containing the four acidic residues, competed with the binding of J18 protein to target cells and significantly inhibited (ϳ60%) the penetration of parasites. This peptide, generated by the juxtaposition of sequences that are separated by a hydrophobic stretch in the linear molecule, appears to be mimicking a conformation-dependent cell-binding site of gp82. Experiments of antibody competition with a set of 20-mer overlapping peptides mapped the epitope for 3F6, a monoclonal antibody directed to gp82 that inhibits parasite invasion, to the sequence represented by peptide p3 (244 to 263), which has a partial overlap with the cell adhesion site.

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Section: Discussionmentioning

confidence: 99%

Characterization of the Cell Adhesion Site ofTrypanosoma cruziMetacyclic Stage Surface Glycoprotein gp82

Manque¹,

Eichinger

Juliano

et al. 2000

Infect Immun

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“…Immunoadorbing exhaustively antitrypomastigote serum with epimastigote forms, an approach described by Zingales et al (1982), was the method employed by these groups to select clones expressing genes coding for proteins compatible with a molecular mass of 85 kDa. Some clones have been fully or partially sequenced: TSA-1 (Fouts et al 1991); Tt34c1 (Takle & Cross 1991); SA85-1.1, 1.2, 1.3 (Kahn et al 1991) and pTt21 (Takle et al 1992). After these first disclosures, other groups mentioned in Table III found genes having high degree of homology with the previously cloned sequences.…”

Section: The Gp-85 Glycoprotein Familymentioning

confidence: 94%

Relevant glycoconjugates on the surface of Trypanosoma cruzi

Colli

Alves

1999

Mem. Inst. Oswaldo Cruz

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“…Several genes for surface antigens display homology among them and can be grouped in a superfamily designated as the gp85/sialidase multigene family (Takle & Cross 1991, Campetella et al 1992, Cross & Takle 1993, Colli 1993, Araya et al 1994.…”

Section: Gene Organization: Single Copy Genes Multicopy Gene Familiesmentioning

confidence: 99%

The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener

Santos

Cano

Schijman

et al. 1997

Mem. Inst. Oswaldo Cruz

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An 85-kilodalton surface antigen gene family of Trypanosoma cruzi encodes polypeptides homologous to bacterial neuraminidases

Cited by 54 publications

References 49 publications

Characterization of the Cell Adhesion Site ofTrypanosoma cruziMetacyclic Stage Surface Glycoprotein gp82

Characterization of the Cell Adhesion Site ofTrypanosoma cruziMetacyclic Stage Surface Glycoprotein gp82

Relevant glycoconjugates on the surface of Trypanosoma cruzi

The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener

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