The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener

Santos, Marcia Rodrigues dos; Cano, Maria Isabel Nogueira; Schijman, Alejandro G.; Lorenzi, Hernán; Vázquez, Martı́n; Levìn, Mariano J.; Ramı́rez, José Luis; Brandão, Adeílton; Degrave, Wim; Silveira, José Franco da

doi:10.1590/s0074-02761997000600018

Cited by 28 publications

(14 citation statements)

References 48 publications

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“…The molecular karyotype of clone CL Brener consists of 20 chromosomal bands (0.45-3.5 Mb 12 megabase bands (3.5-1.0 Mb), and 8 intermediate bands (0.45-1.0 Mb) . The distribution of the ethidium bromide fluorescence is not the same for all chromosomal bands indicating that most of the bands contain two or more comigrating chromosomes which are not necessarily homologous Henriksson et al 1996;Santos et al 1997). Indeed, the existence of homologous chromosomes which differ substantially in size has been suggested (Henriksson et al 1995Cano et al 1995).…”

Section: Identification Of Markers For Chromosomal Bands XVI and Xviimentioning

confidence: 99%

“…Classical genetic studies on this parasite have been hampered by the existence of many parasite strains with distinct biological and immunological characteristics, the lack of sexual stages, and a high variability in both number and size of chromosomes (Brener 1973;Solari 1995;Cano et al 1995;Henriksson et al 1995Henriksson et al , 1996Santos et al 1997). For this reason, one of the main goals of the recently started T. cruzi Genome Initiative is to construct a physical map of the parasite's genome as a resource for DNA sequence analysis (Hanke et al 1996Zingales et al 1997a;Ferrari et al 1997;Frohme et al 1998).…”

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confidence: 99%

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Physical Mapping of a 670-kb Region of Chromosomes XVI and XVII from the Human Protozoan Parasite Trypanosoma cruzi Encompassing the Genes for Two Immunodominant Antigens

Santos

Lorenzi²,

Porcile³

et al. 1999

Genome Res.

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As part of the Trypanosoma cruzi Genome Initiative, we have mapped a large portion of the chromosomal bands XVI (2.3 Mb) and XVII (2.6 Mb) containing the highly repetitive and immunodominant antigenic gene families h49 and jl8. Restriction mapping of the isolated chromosomal bands and hybridization with chromosome specific gene probes showed that genes h49 and jl8 are located in a pair of size-polymorphic homologous chromosomes. To construct the integrated map of the chromosomes harboring the h49 and jl8 loci, we used YAC, cosmid, and phage overlapping clones, and long range restriction analysis using a variety of probes (i.e., known gene sequences, ESTs, polymorphic repetitive sequences, anonymous sequences, STSs generated from the YAC ends). The total length covered by the YAC contig was approximately 670 kb, and its map agreed and was complementary to the one obtained by long-range restriction fragment analysis. Average genetic marker spacing in a 105 kb region around h49 and jl8 genes was estimated to be 6.2 kb/marker. We have detected some polymorphism in the H49/JL8 antigens-encoding chromosomes, affecting also the coding regions. The physical map of this region, together with the isolation of specific chromosome markers, will contribute in the global effort to sequence the nuclear genome of this parasite.

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Section: Identification Of Markers For Chromosomal Bands XVI and Xviimentioning

confidence: 99%

mentioning

confidence: 99%

Physical Mapping of a 670-kb Region of Chromosomes XVI and XVII from the Human Protozoan Parasite Trypanosoma cruzi Encompassing the Genes for Two Immunodominant Antigens

Santos

Lorenzi²,

Porcile³

et al. 1999

Genome Res.

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“…The development of the pulsedfield gel electrophoresis (PFGE) technique made possible the identification of 20 chromosomal bands ranging from 450 Kb to 3500 Kb for CL Brener, the model clone of the T. cruzi genome initiative (Santos et al 1997, Porcile et al 2003. However, the intensities of different chromosomal bands vary in ethidium bromide stained gels, indicating that the chromosome number may be possibly much higher than 20 (Santos et al 1997).…”

Section: Karyotypingmentioning

confidence: 99%

Trypanosoma cruzi: genetic structure of populations and relevance of genetic variability to the pathogenesis of chagas disease

Macedo

Machado

Oliveira

et al. 2004

Mem. Inst. Oswaldo Cruz

257

196

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“…29 Tulahuén and CL Brener epimastigote chromosomes 33 were separated by Pulse Field Gel Electrophoresis (PFGE) (CHEF DR II Bio-Rad) using standard procedures. [34][35][36] The DNA was transferred onto nylon membranes. The Tc45 radioactive DNA probe was hybridized under high stringency conditions and the membrane was exposed to radioautography.…”

mentioning

confidence: 99%

“…The Tc45 radioactive DNA probe was hybridized under high stringency conditions and the membrane was exposed to radioautography. 34,36 Tc45 sequence analysis. Homologies with other described genes were determined by using the BLAST search algorithm at the NCBI Data Base.…”

mentioning

confidence: 99%

Tc45, a dimorphic Trypanosoma cruzi immunogen with variable chromosomal localization, is calreticulin.

Aguillón

Ferreira²,

Pérez³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

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Abstract. We demonstrate that Tc45, a polypeptide described as an immunogenetically restricted Trypanosoma cruzi antigen in mice, is calreticulin, a dimorphic molecule encoded by genes with variable chromosomal distribution. Previously we showed that IgG from A.SW (H2 s ) mice immunized with T. cruzi trypomastigotes or epimastigotes and sera from infected humans recognize Tc45, a 45 kD parasite polypeptide. Herein we describe the cloning, sequencing, and expression of the Tc45 gene. A 98% homology in the deduced amino acid sequence was found with a T. cruzi calreticulin-like molecule and 41% with Leishmania donovani and human calreticulin. In the T. cruzi CL Brener clone and in the Tulahuén strain, the gene is located in two and four chromosomes, respectively. Calreticulin was detected in several T. cruzi clones, in the Tulahuén strain, and in T. rangeli, displaying alternative 43 and 46 kD forms.

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The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener

Cited by 28 publications

References 48 publications

Physical Mapping of a 670-kb Region of Chromosomes XVI and XVII from the Human Protozoan Parasite Trypanosoma cruzi Encompassing the Genes for Two Immunodominant Antigens

Physical Mapping of a 670-kb Region of Chromosomes XVI and XVII from the Human Protozoan Parasite Trypanosoma cruzi Encompassing the Genes for Two Immunodominant Antigens

Trypanosoma cruzi: genetic structure of populations and relevance of genetic variability to the pathogenesis of chagas disease

Tc45, a dimorphic Trypanosoma cruzi immunogen with variable chromosomal localization, is calreticulin.

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