An ABC transporter is required for alkaline stress and potassium transport regulation in<i>Sinorhizobium meliloti</i>

Lin, Dong Xu; Tang, Hui; Wang, En Tao; Chen, Wen Xin

doi:10.1111/j.1574-6968.2009.01500.x

Cited by 15 publications

(7 citation statements)

References 31 publications

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“…The ABC superfamily proteins mediate the transport across biological membranes not only as ATP-dependent pumps but also as ion channels and channel regulators [14]. Previous reports have suggested that ABC transporters in soybean and tomato are involved in salt stress responses and auxin transport [15,16], Seven ABC transporter proteins increased in expression in our study, suggesting that a high expression of ABC transporters in alligator weed stems are required for potassium transport regulation and LK responses [17,18]. However, further research is required to clarify the details of their function.…”

Section: Discussionsupporting

confidence: 55%

Physiological Analysis and Proteome Quantification of Alligator Weed Stems in Response to Potassium Deficiency Stress

Lyu

et al. 2019

IJMS

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The macronutrient potassium is essential to plant growth, development and stress response. Alligator weed (Alternanthera philoxeroides) has a high tolerance to potassium deficiency (LK) stress. The stem is the primary organ responsible for transporting molecules from the underground root system to the aboveground parts of the plant. However, proteomic changes in response to LK stress are largely unknown in alligator weed stems. In this study, we investigated the physiological and proteomic changes in alligator weed stems under LK stress. First, the chlorophyll and soluble protein content and SOD and POD activity were significantly altered after 15 days of LK treatment. The quantitative proteomic analysis suggested that a total of 296 proteins were differentially abundant proteins (DAPs). The functional annotation analysis revealed that LK stress elicited complex proteomic alterations that were involved in oxidative phosphorylation, plant-pathogen interactions, glycolysis/gluconeogenesis, sugar metabolism, and transport in stems. The subcellular locations analysis suggested 104 proteins showed chloroplastic localization, 81 proteins showed cytoplasmic localization and 40 showed nuclear localization. The protein–protein interaction analysis revealed that 56 proteins were involved in the interaction network, including 9 proteins involved in the ribosome network and 9 in the oxidative phosphorylation network. Additionally, the expressed changes of 5 DAPs were similar between the proteomic quantification analysis and the PRM-MS analysis, and the expression levels of eight genes that encode DAPs were further verified using an RT-qPCR analysis. These results provide valuable information on the adaptive mechanisms in alligator weed stems under LK stress and facilitate the development of efficient strategies for genetically engineering potassium-tolerant crops.

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Section: Discussionsupporting

confidence: 55%

Physiological Analysis and Proteome Quantification of Alligator Weed Stems in Response to Potassium Deficiency Stress

Lyu

et al. 2019

IJMS

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“…In contrast, accumulation of RNA corresponding to SupAB (SMb20484-5) decreased in the hfq mutant. The SupAB systems participates in alkaline tolerance, possibly by controlling potassium uptake under high pH (Lin et al 2009). Differential accumulation of actSR and supAB mRNAs was confirmed by qPCR (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Role of the Sinorhizobium meliloti Global Regulator Hfq in Gene Regulation and Symbiosis

Gao¹,

Barnett²,

Long³

et al. 2010

MPMI

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The RNA-binding protein Hfq is a global regulator which controls diverse cellular processes in bacteria. To begin understanding the role of Hfq in the Sinorhizobium meliloti–Medicago truncatula nitrogen-fixing symbiosis, we defined free-living and symbiotic phenotypes of an hfq mutant. Over 500 transcripts were differentially accumulated in the hfq mutant of S. meliloti Rm1021 when grown in a shaking culture. Consistent with transcriptome-wide changes, the hfq mutant displayed dramatic alterations in metabolism of nitrogen-containing compounds, even though its carbon source utilization profiles were nearly identical to the wild type. The hfq mutant had reduced motility and was impaired for growth at alkaline pH. A deletion of hfq resulted in a reduced symbiotic efficiency, although the mutant was still able to initiate nodule development and differentiate into bacteroids.

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“…Whereas some Mycobacterium cells survived pH values of < 1 and > 13, only a small number of acidophilic Archaea can live in the pH range 2 to 0 (Golyshina, ), and no microbe is capable of growth above pH 12 (Rummel et al ., ). By contrast to the mycobacteria tested, viability is reduced to low levels for S. aureus at pH values of 3.5 and 10 (Cebrián et al ., ); for Enterococcus faecalis at pH 4.8 or 10.5 (Flahaut et al ., ); and for the soil bacterium Sinorhizobium meliloti at pH 10 (Lin et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Extraordinary solute‐stress tolerance contributes to the environmental tenacity of mycobacteria

Santos

Carvalho²,

Stevenson

et al. 2015

Environ Microbiol Rep

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Mycobacteria are associated with a number of well-characterized diseases, yet we know little about their stress biology in natural ecosystems. This study focuses on the isolation and characterization of strains from Yellowstone National Park (YNP) and Glacier National Park (GNP; USA), the majority of those identified were Mycobacterium parascrofulaceum, Mycobacterium avium (YNP) or Mycobacterium gordonae (GNP). Generally, their windows for growth spanned a temperature range of > 60 °C; selected isolates grew at super-saturated concentrations of hydrophobic stressors and at levels of osmotic stress and chaotropic activity (up to 13.4 kJ kg(-1) ) similar to, or exceeding, those for the xerophilic fungus Aspergillus wentii and solvent-tolerant bacterium Pseudomonas putida. For example, mycobacteria grew down to 0.800 water activity indicating that they are, with the sole exception of halophiles, more xerotolerant than other bacteria (or any Archaea). Furthermore, the fatty-acid composition of Mycobacterium cells grown over a range of salt concentrations changed less than that of other bacteria, indicating a high level of resilience, regardless of the stress load. Cells of M. parascrofulaceum, M. smegmatis and M. avium resisted the acute, potentially lethal challenges from extremes of pH (< 1; > 13), and saturated MgCl2 solutions (5 M; 212 kJ kg(-1) chaotropicity). Collectively, these findings challenge the paradigm that bacteria have solute tolerances inferior to those of eukaryotes.

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An ABC transporter is required for alkaline stress and potassium transport regulation inSinorhizobium meliloti

Cited by 15 publications

References 31 publications

Physiological Analysis and Proteome Quantification of Alligator Weed Stems in Response to Potassium Deficiency Stress

Physiological Analysis and Proteome Quantification of Alligator Weed Stems in Response to Potassium Deficiency Stress

Role of the Sinorhizobium meliloti Global Regulator Hfq in Gene Regulation and Symbiosis

Extraordinary solute‐stress tolerance contributes to the environmental tenacity of mycobacteria

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