“…The passive enhancing method (Long et al, 2009;Schönfeld et al, 2004;Zhang et al, 2010) mainly utilizes the energy from pumps and improves mixing by enhancing relative movement of fluid particles, which results in complex microchannel structure as well as possible dead volume. The active enhancing method, in contrast, is working with the help of extra energy input, such as ultrasound (Yaralioglu et al, 2004), piezoelectrically vibrating (Woias et al, 2000), electric field (Oddy et al, 2001), pressure pulse (Glasgow and Aubry, 2003) etc. Other than these direct energy input approaches, the introduction of inert fluid is another simple but effective method to intensify mixing process.…”