An Activity‐Based Ratiometric Fluorescent Probe for In Vivo Real‐Time Imaging of Hydrogen Molecules

Gong, Wanjun; Jiang, Lingdong; Zhu, Yanxia; Jiang, Mengna; Chen, Danyang; Jin, Zhaokui; Qin, Shucun; Yu, Zhiqiang; He, Qianjun

doi:10.1002/anie.202114594

Cited by 29 publications

(24 citation statements)

References 34 publications

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“…The administration of antioxidants for scavenging ROS is recognized to be a promising strategy of arthritis treatment (7)(8)(9). Among various antioxidants, hydrogen molecule has broad-spectrum antioxidation and anti-inflammation effects, outstanding tissue penetration ability, and high biosafety against a variety of inflammationrelated diseases including arthritis (10)(11)(12)(13)(14)(15). Sung et al and Li et al (16,17) locally injected the Mg powder-encapsulated poly(lacticco-glycolic acid) microparticles into mouse arthritis knee to deliver hydrogen molecules for inflammation mitigation, arresting the progression of arthritis.…”

Section: Introductionmentioning

confidence: 99%

NIR-photocatalytic regulation of arthritic synovial microenvironment

et al. 2022

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Synovial microenvironment (SME) plays a vital role in the formation of synovial pannus and the induction of cartilage destruction in arthritis. In this work, a concept of the photocatalytic regulation of SME is proposed for arthritis treatment, and monodispersive hydrogen–doped titanium dioxide nanorods with a rutile single-crystal structure are developed by a full-solution method to achieve near infrared–photocatalytic generation of hydrogen molecules and simultaneous depletion of overexpressed lactic acid (LA) for realizing SME regulation in a collagen-induced mouse model of rheumatoid arthritis. Mechanistically, locally generated hydrogen molecules scavenge overexpressed reactive oxygen species to mediate the anti-inflammatory polarization of macrophages, while the simultaneous photocatalytic depletion of overexpressed LA inhibits the inflammatory/invasive phenotypes of synoviocytes and macrophages and ameliorates the abnormal proliferation of synoviocytes, thereby remarkably preventing the synovial pannus formation and cartilage destruction. The proposed catalysis-mediated SME regulation strategy will open a window to realize facile and efficient arthritis treatment.

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Section: Introductionmentioning

confidence: 99%

NIR-photocatalytic regulation of arthritic synovial microenvironment

et al. 2022

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“…The diffusion of H 2 is an important factor for destroying the internal structure of biofilm, so we further explored the permeation of H 2 in bacterial biofilm under confocal microscope imaging taking advantage of our newly-developed ratiometric fluorescent hydrogen nano-probe (NDI-N 3 /Pd@MSN-PEG) [ 42 ]. The nano-probe was uniformly dispersed and fixed into S.a. bacterial biofilm, and then saturated hydrogen-rich water was added on the biofilm followed by confocal microscope tomoscan imaging.…”

Section: Resultsmentioning

confidence: 99%

Sonocatalytic hydrogen/hole-combined therapy for anti-biofilm and infected diabetic wound healing

Chen

Jiang

et al. 2023

National Science Review

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It is a great challenge to effectively eradicate biofilm and cure biofilm-infected diseases because dense extracellular polymeric substance matrix prevents routine antibacterial agents from penetrating into biofilm. H2 is an emerging energy-regulating molecule possessing both high biosafety and high tissue permeability. In this work, we propose a concept of sonocatalytic hydrogen/hole-combined ‘inside/outside-cooperation’ anti-biofilm for promoting bacteria-infected diabetic wound healing based on piezoelectric two-dimensional nanomaterials. Proof-of-concept experiments using C3N4 nanosheets as a representative piezoelectric catalyst with wide band gap and high biosafety have verified that sonocatalytically generated H2 and holes rapidly penetrate into biofilm to inhibit bacterial energy metabolism and oxidatively deprive polysaccharides/NADH in biofilm to destroy the bacterial membrane/electron transport chain, respectively, inside/outside-cooperatively eradicating biofilm. A bacteria-infected diabetic wound model is used to confirm the excellent in vivo anti-bacterial performance of sonocatalytic hydrogen/hole-combined therapy, remarkably improving bacteria-infected diabetic wound healing. The proposed strategy of sonocatalytic hole/hydrogen-combined ‘inside/outside-cooperation’ will break a highway for treatment of deep-seated biofilm infection.

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“…Ratiometric fluorescence imaging is a method where intensities at two or more different wavelengths are measured to detect the dynamic behavior and function relationships of biomolecules in living organisms [ 17 , 18 , 19 ]. Ratiometric fluorescence imaging provides built-in self-calibration for factors unrelated to the analyte (e.g., variable probe concentration and distribution, instrument sensitivity, dynamic biological environment and photobleaching), thus improving the reliability of biomolecular detection [ 20 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Ratiometric Near-Infrared Fluorescence Liposome Nanoprobe for H2S Detection In Vivo

Liu

Zhang

et al. 2023

Molecules

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Accurate detection of H2S is crucial to understanding the occurrence and development of H2S-related diseases. However, the accurate and sensitive detection of H2S in vivo still faces great challenges due to the characteristics of H2S diffusion and short half-life. Herein, we report a H2S-activatable ratiometric near-infrared (NIR) fluorescence liposome nanoprobe HS-CG by the thin-film hydration method. HS-CG shows “always on” fluorescence signal at 816 nm and low fluorescence signal at 728 nm; the NIR fluorescence ratio between 728 and 816 nm (F728/F816) is low. Upon reaction with H2S, the fluorescence at 728 nm could be more rapidly turned on due to strong electrostatic interaction between enriched HS− and positively charged 1,2-dihexadecanoyl-sn-glycero-3-phosphocholine (DPPC) doped in the liposome nanoprobe HS-CG, resulting in a large enhancement of F728/F816, which allows for sensitive visualization of the tumor H2S levels in vivo. This study demonstrates that this strategy of electrostatic adsorption between HS− and positively charged molecules provides a new way to enhance the reaction rate of the probe and H2S, thus serving as an effective platform for improving the sensitivity of imaging.

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An Activity‐Based Ratiometric Fluorescent Probe for In Vivo Real‐Time Imaging of Hydrogen Molecules

Cited by 29 publications

References 34 publications

NIR-photocatalytic regulation of arthritic synovial microenvironment

NIR-photocatalytic regulation of arthritic synovial microenvironment

Sonocatalytic hydrogen/hole-combined therapy for anti-biofilm and infected diabetic wound healing

Ratiometric Near-Infrared Fluorescence Liposome Nanoprobe for H2S Detection In Vivo

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