An advanced method for the determination of carboxyl methyl esterase activity using gas chromatography–chemical ionization–mass spectrometry

Koo, Yeon Jong; Yoon, Eunsil; Song, Jong Tae; Seo, Hak Soo; Kim, Jeong‐Han; Lee, Yin-Won; Lee, Jong Seob; Cheong, Jong-Joo; Choi, Yang Do

doi:10.1016/j.jchromb.2008.01.015

Cited by 15 publications

(3 citation statements)

References 23 publications

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“…Jasmonates were extracted from 1 g of leaf tissue ground into a fine powder. The jasmonates were quantified by GC/ mass spectrometry (Koo et al 2008). Tuberonic acid was quantified according to the procedure of Gidda et al (2003).…”

Section: Quantification Of Jasmonatesmentioning

confidence: 99%

Overexpression of jasmonic acid carboxyl methyltransferase increases tuber yield and size in transgenic potato

Sohn

Lee

Seo

et al. 2010

Plant Biotechnol Rep

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Jasmonates control diverse plant developmental processes, such as seed germination, flower, fruit and seed development, senescence and tuberization in potato. To understand the role of methyl jasmonate (MeJA) in potato tuberization, the Arabidopsis JMT gene encoding jasmonic acid carboxyl methyltransferase was constitutively overexpressed in transgenic potato plants. Increases in tuber yield and size as well as in vitro tuberization frequency were observed in transgenic plants. These were correlated with JMT mRNA level--the higher expression level, the higher the tuber yield and size. The levels of jasmonic acid (JA), MeJA and tuberonic acid (TA) were also higher than those in control plants. Transgenic plants also exhibited higher expression of jasmonate-responsive genes such as those for allene oxide cyclase (AOC) and proteinase inhibitor II (PINII). These results indicate that JMT overexpression induces jasmonate biosynthesis genes and thus JA and TA pools in transgenic potatoes. This results in enhanced tuber yield and size in transgenic potato plants.

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Section: Quantification Of Jasmonatesmentioning

confidence: 99%

Overexpression of jasmonic acid carboxyl methyltransferase increases tuber yield and size in transgenic potato

Sohn

Lee

Seo

et al. 2010

Plant Biotechnol Rep

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“…Therefore, carboxylesterase assays would benefit various biochemical studies for understanding its mechanism of action and role in drug metabolism. A number of strategies have been established for carboxylesterase detection with the application of chromatography,6 mass spectrometry,7 chemiluminescence,8 and fluorescence 9. Among them, biomolecular probes based on different fluorogenic substrates have received much attention due to the simplicity, speed, and sensitivity of their assays 10.…”

Section: Introductionmentioning

confidence: 99%

A Fluorogenic Probe with Aggregation‐Induced Emission Characteristics for Carboxylesterase Assay through Formation of Supramolecular Microfibers

Wang

Liu

et al. 2014

Chemistry An Asian Journal

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Herein, we report a novel fluorescent "light-up" probe useful for carboxylesterase assay that is based on a tetraphenylethylene derivative containing carboxylic ester groups. The specific cleavage of the carboxylic ester bonds by carboxylesterase results in the generation of a relatively hydrophobic moiety that self-assembles into supramolecular microfibers, thus giving rise to "turn-on" fluorescent signals. A high sensitivity towards carboxylesterase was achieved with a detection limit as low as 29 pM, which is much lower than the corresponding assays based on other fluorescent approaches.

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“…Some analytical methods have been reported for the quantitative determination of jasmonic acid, including radioimmunoassay, enzyme-linked immunosorbent assay (ELISA), , gas chromatography–mass spectrometry (GC/MS), − liquid chromatography–mass spectrometry (LC/MS) or liquid chromatography–tandem mass spectrometry (LC/MS/MS), , high-performance liquid chromatography coupled with fluorescence detection (HPLC-FD), ,, capillary electrophoresis–mass spectrometry (CE-MS), and capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) . It has been reported that GC/MS and ELISA are well suitable for the assay of large numbers of samples and exhibit good selectivity for jasmonic acid. , However, these methods have some inherent problems.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Determination of Jasmonic Acid in Plant Tissues Using High-Performance Liquid Chromatography with Fluorescence Detection

Xiong

Rao

Guo

et al. 2012

J. Agric. Food Chem.

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An ultrasensitive and selective high-performance liquid chromatographic method for the volatile signaling hormone, jasmonic acid, has been developed based on precolumn derivatization with 1,3,5,7-tetramethyl-8-aminozide-difluoroboradiaza-s-indacene (BODIPY-aminozide). The derivatization reaction was carried out at 60 °C for 30 min in the presence of phosphoric acid. The formed jasmonic acid derivative was eluted using a mobile phase of methanol/pH 6.50 ammonium formate buffer/tetrahydrofuran (67:30:3, v/v/v) in 10 min on a C(18) column and detected with fluorescence detection at excitation and emission wavelengths of 495 and 505 nm, respectively. The detection limit (signal-to-noise ratio = 4) reached 1.14 × 10(-10) M or 2.29 fmol per injection (20 μL), which is the lowest of the existing methods. The proposed method has been successfully applied to the direct determination of trace jasmonic acid in the crude extracts of soybean leaves from soybean mosaic virus-infected and normal plants with recoveries of 95-104%.

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An advanced method for the determination of carboxyl methyl esterase activity using gas chromatography–chemical ionization–mass spectrometry

Cited by 15 publications

References 23 publications

Overexpression of jasmonic acid carboxyl methyltransferase increases tuber yield and size in transgenic potato

Overexpression of jasmonic acid carboxyl methyltransferase increases tuber yield and size in transgenic potato

A Fluorogenic Probe with Aggregation‐Induced Emission Characteristics for Carboxylesterase Assay through Formation of Supramolecular Microfibers

Ultrasensitive Determination of Jasmonic Acid in Plant Tissues Using High-Performance Liquid Chromatography with Fluorescence Detection

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