An aligned nanofibrous collagen scaffold by electrospinning and its effects on <i>in vitro</i> fibroblast culture

Zhong, Shian; Teo, Wee Eong; Zhu, Xin; Beuerman, Roger W.; Ramakrishna, Seeram; Yung, Lin‐Yue Lanry

doi:10.1002/jbm.a.30870

Cited by 318 publications

(231 citation statements)

References 26 publications

(38 reference statements)

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“…83 Schwan cells aligned along the PCL fibers and elongated along the orientation of the fibers, suggesting that the fibers guided the cell alignment and spreading. 144 Similar alignment has also been shown in fibroblasts 145 and endothelial cells. 146 Thus, in tissues where ordered cell alignment is necessary, fibers can be oriented by spinning the collector plate or with different void spaces.…”

Section: Cellular Interactionssupporting

confidence: 75%

Next Generation of Electrosprayed Fibers for Tissue Regeneration

Hong

Madihally

2011

Tissue Engineering Part B: Reviews

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Electrospinning is a widely established polymer-processing technology that allows generation of fibers (in nanometer to micrometer size) that can be collected to form nonwoven structures. By choosing suitable process parameters and appropriate solvent systems, fiber size can be controlled. Since the technology allows the possibility of tailoring the mechanical properties and biological properties, there has been a significant effort to adapt the technology in tissue regeneration and drug delivery. This review focuses on recent developments in adapting this technology for tissue regeneration applications. In particular, different configurations of nozzles and collector plates are summarized from the view of cell seeding and distribution. Further developments in obtaining thick layers of tissues and thin layered membranes are discussed. Recent advances in porous structure spatial architecture parameters such as pore size, fiber size, fiber stiffness, and matrix turnover are summarized. In addition, possibility of developing simple three-dimensional models using electrosprayed fibers that can be utilized in routine cell culture studies is described. Tissue EngineeringT issue engineering or regeneration is a multidisciplinary study to restore, maintain, and enhance tissue and organ function. 1 In tissue engineering, biodegradable scaffolds are used to support and guide cells to proliferate, organize, and produce their own extracellular matrix (ECM). Scaffolding material eventually disappears leaving only the necessary healthy tissue in a topologically required form. 2,3 Assembly and maturation of ECM elements is important in determining the biomechanics and the quality of the tissue. For example, collagen provides tensile strength to the tissue, elastic fibers contribute to the elasticity of the tissue, while proteoglycans fills the extracellular space, creating a space for the tissue regulation of growth factors and other interactions. 4 Delicate balance between different matrix elements is necessary to generate healthy tissue. The size and shape of collagen fibers in ECM relies on tissues and organs even in the same species. The shape is cord or tape with a width of 1-20 mm and the collagen fibrils (unit can be observed by electron microscopy) are cylindrical with a diameter ranging from 10 to over 500 nm where cell is attaching and hugging. 5 Obtaining a biodegradable matrix conducive for cell colonization is a fundamental requirement for tissue regeneration. Like ECM, biomimic scaffold should allow cell attachment and migration, enables diffusion of vital cell nutrients, and retains cells. Further, chemical and mechanical properties of scaffold influence cell viability and proliferation. 6 Appropriate pore size and porosity are essential to modulate cell seeding and diffusion. Biodegradability is essential because scaffolds are absorbed and distributed to the nearby tissue, which allows no surgical removal from body. The surface of scaffold is suitable for cell attachment and migration. The mechanical properties...

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Section: Cellular Interactionssupporting

confidence: 75%

Next Generation of Electrosprayed Fibers for Tissue Regeneration

Hong

Madihally

2011

Tissue Engineering Part B: Reviews

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“…An assortment of natural polymers, such as the proteins collagen, elastin and silk fibroin, has been electrospun [25][26][27][28][29][30][31]. In addition, a wide variety of synthetic polymers such as polystyrene (PS), polycaprolactone (PCL), poly(methyl methacrylate) (PMMA) and poly(vinyl alcohol) (PVA) have been electrospun from solution.…”

Section: Introductionmentioning

confidence: 99%

Hierarchically Structured Electrospun Fibers

Zander

2013

Polymers

130

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Abstract:Traditional electrospun nanofibers have a myriad of applications ranging from scaffolds for tissue engineering to components of biosensors and energy harvesting devices. The generally smooth one-dimensional structure of the fibers has stood as a limitation to several interesting novel applications. Control of fiber diameter, porosity and collector geometry will be briefly discussed, as will more traditional methods for controlling fiber morphology and fiber mat architecture. The remainder of the review will focus on new techniques to prepare hierarchically structured fibers. Fibers with hierarchical primary structures-including helical, buckled, and beads-on-a-string fibers, as well as fibers with secondary structures, such as nanopores, nanopillars, nanorods, and internally structured fibers and their applications-will be discussed. These new materials with helical/buckled morphology are expected to possess unique optical and mechanical properties with possible applications for negative refractive index materials, highly stretchable/high-tensile-strength materials, and components in microelectromechanical devices. Core-shell type fibers enable a much wider variety of materials to be electrospun and are expected to be widely applied in the sensing, drug delivery/controlled release fields, and in the encapsulation of live cells for biological applications. Materials with a hierarchical secondary structure are expected to provide new superhydrophobic and self-cleaning materials.

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“…16 Fibroblasts have also been shown to co-align and polarize along the axial direction of collagen fibers, whereas fibroblasts adhered to randomly oriented collagen fibers have an anisotropic orientation. 17 Perhaps even more significant, increased proliferation of fibroblasts adhered to aligned collagen scaffolds compared to the random scaffold was shown, with fibroblast morphology on aligned collagen comparing well with that seen in native tissues. 18 Elias et al showed that osteoblasts proliferate significantly more on a network of »100 nm diameter carbon fibers rather than on flat glass.…”

Section: Local Topology Effect On Cellsmentioning

confidence: 79%

Elucidating the molecular mechanisms underlying cellular response to biophysical cues using synthetic biology approaches

Denning

Roos

2016

Cell Adhesion & Migration

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The use of synthetic surfaces and materials to influence and study cell behavior has vastly progressed our understanding of the underlying molecular mechanisms involved in cellular response to physicochemical and biophysical cues. Reconstituting cytoskeletal proteins and interfacing them with a defined microenvironment has also garnered deep insight into the engineering mechanisms existing within the cell. This review presents recent experimental findings on the influence of several parameters of the extracellular environment on cell behavior and fate, such as substrate topography, stiffness, chemistry and charge. In addition, the use of synthetic environments to measure physical properties of the reconstituted cytoskeleton and their interaction with intracellular proteins such as molecular motors is discussed, which is relevant for understanding cell migration, division and structural integrity, as well as intracellular transport. Insight is provided regarding the next steps to be taken in this interdisciplinary field, in order to achieve the global aim of artificially directing cellular response.

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An aligned nanofibrous collagen scaffold by electrospinning and its effects on in vitro fibroblast culture

Cited by 318 publications

References 26 publications

Next Generation of Electrosprayed Fibers for Tissue Regeneration

Next Generation of Electrosprayed Fibers for Tissue Regeneration

Hierarchically Structured Electrospun Fibers

Elucidating the molecular mechanisms underlying cellular response to biophysical cues using synthetic biology approaches

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