An allosteric model for the binding of bilirubin to the bilirubin imprinted poly(methacrylic acid-co-ethylene glycol dimethylacrylate)

Syu, Mei-Jywan; Nian, You Ming

doi:10.1016/j.aca.2005.03.031

Cited by 28 publications

(9 citation statements)

References 23 publications

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“…The demonstration of template (or template analog)‐selective recognition by an MIP remains a cornerstone in the development of all new MIP systems. Various strategies have been employed to characterize ligand‐polymer binding events,…”

Section: Fundamental Aspectsmentioning

confidence: 99%

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

2014

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SummaryHerein we present a survey of the literature covering the development of molecular imprinting science and technology over the years 2004 to 2011. In total, 3779 references to the original papers, reviews, edited volumes and monographs from this period are included, along with recently identified uncited materials from prior to 2004 which were omitted in the first instalment of this series covering the years 1930 to 2003. 1 In the presentation of the assembled references, a section presenting reviews and monographs covering the area is followed by sections describing fundamental aspects of molecular imprinting including the development of novel polymer formats. Thereafter, literature describing efforts to apply these polymeric materials to a range of application areas is presented. Current trends and areas of rapid development are discussed.2

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Section: Fundamental Aspectsmentioning

confidence: 99%

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

2014

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“…However, the adsorption capacity of MIP particles was also good, when compared to other MIP adsorbents. [30][31][32][33] Differences of bilirubin adsorption capacity are due to the properties of each adsorbent, such as structure, functional groups, ligand loading, and accessible surface area.…”

Section: Effect Of Equilibrium Concentration Of Bilirubinmentioning

confidence: 99%

Selective Removal of Bilirubin from Human Plasma with Bilirubin-Imprinted Particles

Baydemir

Andaç

Bereli

et al. 2007

Ind. Eng. Chem. Res.

129

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The objective of this study is to prepare bilirubin-imprinted polymeric particles for the selective removal of bilirubin from hyperbilirubinemic human plasma. N-methacryloyl-(L)-tyrosine methylester (MAT) was chosen as the complexing monomer. In the first step, functional monomer MAT was synthesized by the reaction of L-tyrosine methylester and methacryloyl chloride and characterized by nuclear magnetic resonance (NMR). Bilirubin then was complexed with MAT and the bilirubin-imprinted poly(2-hydroxyethyl methacrylate-Nmethacryloyl-(L)-tyrosine methylester) [MIP] was produced by bulk polymerization. The template molecules (i.e., bilirubin) then were removed using sodium carbonate and sodium hydroxide. MIP particles were characterized by elemental analysis, water uptake tests, Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM). Bilirubin adsorption experiments from human plasma were performed in a batch experimental setup. Cholesterol and testosterone were used as competing molecules in selectivity tests. Obtained results were as follows: the water uptake ratio of MIP and non-imprinted (NIP) particles were 64.7% and 51.3%, respectively, in water. According to the elemental analysis results, the incorporation of MAT was 69.0 µmol/g for MIP particles. SEM micrographs showed the surface roughness and porosity. The specific surface area of the MIP particles was determined to be 27.8 m 2 /g. The pore diameter of the MIP particles varied over a range of 20-245 Å and the average pore diameter was 25.0 Å. Template molecules (i.e., bilirubin) were removed from the polymer structure in the ratio of 87% of the initial concentration. Bilirubin adsorption increased as the bilirubin concentration increased, up to 0.8 mg/mL. The maximum bilirubin adsorption capacity was 3.4 mg/g of the dry weight of particles. MIP particles were 6.3 and 3.0 times more selective, with respect to the cholesterol and testosterone, respectively. Reusability of the MIP particles was also investigated. MIP particles showed a negligible loss in the bilirubin adsorption capacity after five adsorption-desorption cycles with the same adsorbent.

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“…Theoretically, monolayered binding results in specific recognition of the MIPs toward target molecules. However, formation of hydrogen bonds or noncovalent bonds between the analytes or between the MIPs and the analyte molecules might also occur . In this work, the Freundlich model did not fit the equilibrium data but did not exclude the possibility of a multilayered binding.…”

Section: Resultsmentioning

confidence: 82%

“…In fact, the Freundlich model did not take into account the effect of the interactions between the different analytes, which should be considered when establishing the binding model in the presented systems herein. The allosteric model is proposed to model the multilayer binding behavior of the MIPs as represented in the Equation . The model has been successfully applied for the description of the specific binding behavior of the different MIPs toward their templates .…”

Section: Resultsmentioning

confidence: 99%

Synthesis of molecularly imprinted polymers using acrylamide‐β‐cyclodextrin as a cofunctional monomer for the specific capture of tea saponins from the defatted cake extract of Camellia oleifera

Guo

Xiong

et al. 2016

J of Separation Science

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Molecularly imprinted polymers were synthesized using mixed tea saponins as a template and acrylamide-β-cyclodextrin as a cofunctional monomer for the specific binding and purification of tea saponins from the defatted cake extract of Camellia oleifera. The adsorption properties of the prepared polymers were systematically evaluated including adsorption kinetics, adsorption isotherms, and selective recognition characteristics. It showed that the adsorption kinetics followed the pseudo first-order kinetic model (R = 0.995) with an equilibrium time of 3 h, adsorption isotherm data fitted well with the Langmuir-Freundlich model (R = 0.984) with an adsorption capacity of 14.23 mg/g. The relative selectivity coefficient (k´) in the presence of the analogues glycyrrhizic acid and glycyrrhetinic acid were 1.16 and 17.21, respectively. The performance of the molecularly imprinted polymers as solid-phase extraction materials was investigated and the results indicated that using acrylamide-β-cyclodextrin as a cofunctional monomer improved both the adsorption capacity and active sites stability of the imprinted polymers. The solid-phase extraction using the polymers as packing materials was subsequently applied for the separation of tea saponins in raw C. oleifera press extract, and targets were obtained with a purity reaching 89%.

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An allosteric model for the binding of bilirubin to the bilirubin imprinted poly(methacrylic acid-co-ethylene glycol dimethylacrylate)

Cited by 28 publications

References 23 publications

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

Selective Removal of Bilirubin from Human Plasma with Bilirubin-Imprinted Particles

Synthesis of molecularly imprinted polymers using acrylamide‐β‐cyclodextrin as a cofunctional monomer for the specific capture of tea saponins from the defatted cake extract of Camellia oleifera

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