1997
DOI: 10.1006/bbrc.1997.6817
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An Alternative Form of Nucleolysin Binds to a T-Cluster DNA in the Silencer Element of Platelet Factor 4 Gene

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Cited by 8 publications
(9 citation statements)
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“…The cell pellet was washed with CATCH medium and centrifuged again. The cell pellet was resuspended in lysis buffer (140 mM NH 4 Cl, 17 mM Tris-HCl [pH 7.2]) and incubated at 37°C for 10 minutes. Cells were centrifuged and washed twice with CATCH buffer.…”
Section: Preparation Of Rat Megakaryocytesmentioning
confidence: 99%
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“…The cell pellet was washed with CATCH medium and centrifuged again. The cell pellet was resuspended in lysis buffer (140 mM NH 4 Cl, 17 mM Tris-HCl [pH 7.2]) and incubated at 37°C for 10 minutes. Cells were centrifuged and washed twice with CATCH buffer.…”
Section: Preparation Of Rat Megakaryocytesmentioning
confidence: 99%
“…4 We compared the sequences of this region in the rat PF4 promoter to those in the human and mouse PF4 promoters and found that homologous sequences to the rat TME exist in both human and mouse ( Figure 9A). EMSA using these TME-like sequences as competitors showed that MEIS1/PBX Analysis of TME binding proteins in rat megakaryocytes.…”
Section: Meis1/pbx Complexes Synergistically Activate Pf4 Gene Expresmentioning
confidence: 99%
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“…1 To investigate megakaryocytic gene expression, the rat and human PF4 promoter have been studied. [2][3][4][5][6][7][8][9][10] We previously demonstrated that GATA-1 and ETS-1 are important transcription factors for the transactivation of the PF4 gene. 5 More recently, we identified the novel regulatory element termed "TME" in the PF4 promoter and demonstrated that homeodomain proteins, myeloid ecotropic integration site 1 (MEIS1) and pre-B-cell leukemia transcription factors (PBXs) (PBX1B and PBX2), bind to the TGACAG motifs in the tandem repeat of MEIS1 binding element (TME) by forming MEIS1/PBX complexes.…”
Section: Introductionmentioning
confidence: 99%
“…In the human, the TIAL1 gene was previously assigned to the chromosome 10 radiation map (under the name TCBP), close to WI-5255 (Doi et al, 1997), itself close to the marker D10S209, linked to the gene FGFR2 (Dib et al, 1996 and http://www. ncbi.nlm.nih.gov/genemap) cytogenetically mapped near the 10q telomere, at 10q25→ q26 (GDB and references therein).…”
Section: Resultsmentioning
confidence: 99%