2012
DOI: 10.1016/j.bej.2012.07.008
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An amperometric biosensor based on laccase immobilized onto nickel nanoparticles/carboxylated multiwalled carbon nanotubes/polyaniline modified gold electrode for determination of phenolic content in fruit juices

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Cited by 64 publications
(34 citation statements)
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“…The solid laccase sample was dissolved in deionized water at concentration of 20 mg mL -1 . The laccase was 9 immobilized onto the surface of GF through hydrophobic or electrostatics interactions by layering 50 uL of enzyme solution in the condition of undisturbed at 4 o C for about 2h [16]. Aniline monomer was purified through distillation under reduced pressure before use.…”
Section: Gf Electrode Modificationmentioning
confidence: 99%
See 1 more Smart Citation
“…The solid laccase sample was dissolved in deionized water at concentration of 20 mg mL -1 . The laccase was 9 immobilized onto the surface of GF through hydrophobic or electrostatics interactions by layering 50 uL of enzyme solution in the condition of undisturbed at 4 o C for about 2h [16]. Aniline monomer was purified through distillation under reduced pressure before use.…”
Section: Gf Electrode Modificationmentioning
confidence: 99%
“…Laccases are the multicopper oxidases capable of catalyzing the oxidation of various organic and inorganic substrates at the presence of molecular oxygen as oxidant [16][17][18][19][20]. Recently, laccases were widely used as a biocatalyst to assist the polymerization of such monomer as aniline [21][22][23].…”
Section: Introductionmentioning
confidence: 99%
“…The other is that the composite of CNTs/PANI is prepared in advance. Subsequently, the composite is fixed directly on the surface of the bare electrode by dropping and dipping method [23][24][25][26]. However, these ways may change the original novel nanometer hollow tube structure of the CNTs due to adding electrode materials (for example, the binder), which may decrease the utilization efficiency of the CNTs and increase the resistance of the composite.…”
Section: Introductionmentioning
confidence: 99%
“…According to current research reports [15][16][17][18][19][20][21][22][23][24][25][26], the CNTs/PANI chemically modified electrodes (CNTs/PANI-CMEs) can be prepared by two ways. One is fixing functionalized CNTs on the surface of the bare electrode by dropping and dipping method, then growing PANI on the CNTs by chemical oxidation or electrochemical polymerization [19][20][21][22].…”
Section: Introductionmentioning
confidence: 99%
“…For example a biosensor using nickel nanoparticles (NiNPs)/carboxylated multiwalled carbon nanotubes/polyaniline composite was developed for phenol detection in fruit juice. The results show the electrodeposition of NiNPs onto the electrode surface caused to increase in current intensity due to increase of electroactive area [23]. Likewise, nanomaterials in a lot of sensors and biosensors have an important role to increase of the response sensitivity [24][25][26][27][28][29].…”
Section: Introductionmentioning
confidence: 99%