An Anabolic Signaling Response of Rat Soleus Muscle to Eccentric Contractions Following Hindlimb Unloading: A Potential Role of Stretch-Activated Ion Channels

Tyganov, Sergey A.; Mirzoev, Timur M.; Шенкман, Борис

doi:10.3390/ijms20051165

Cited by 23 publications

(17 citation statements)

References 42 publications

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“…Thus, chronic contractile activity may modulate the sensitivity of mTORC1 and MPS to muscle contraction. However, recently, Tyganov et al (2019) have observed that the response of mTORC1 activation and MPS to acute muscle contraction is impaired after muscle unloading in soleus muscle. Although the reason for this discrepancy is unclear, experimental conditions such as muscle contraction (in vivo vs. ex vivo muscle contraction), unloading period, and sampling time point after muscle contraction may vary the results.…”

Section: Discussionmentioning

confidence: 99%

Response of Resistance Exercise-Induced Muscle Protein Synthesis and Skeletal Muscle Hypertrophy Are Not Enhanced After Disuse Muscle Atrophy in Rat

et al. 2020

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Skeletal muscle disuse rapidly decreases muscle mass. Resistance training (RT) is believed as the most effective way to gain muscle mass via an increase in mTORC1 activity and muscle protein synthesis (MPS). However, it remains unclear whether muscle atrophy by disuse alters the mTORC1 activation and MPS response to an acute resistance exercise (RE) and chronic RT-mediated skeletal muscle hypertrophy. This study investigated the influence of disuse muscle atrophy on the response of mTORC1 activation and MPS to an acute RE. We also evaluated whether disuse muscle atrophy affects the response of RT-induced muscle mass gain. Thirty male Sprague-Dawley rats were randomly divided into control (CON) or hindlimb suspension (HS) groups. A 14-day HS via the tail was used as the model for gastrocnemius muscle disuse in the HS group. Unilateral lower limb muscle contraction using by percutaneous electrical stimulation was used to mimic the stimuli of RE. Ten bouts of RE were performed in 3-week as chronic RT. Our results showed that MPS and mTORC1 activity was unchanged after HS at basal state. However, the ribosomal RNA (rRNA) level was reduced in HS rats compared to that in CON rats at basal state. MPS and rRNA increased in both HS and CON rats in response to acute RE to the same extent. However, the level of mTORC1 activation in response to an acute RE was significantly higher in HS than that in the CON group at 12 h after exercise, even though no difference was observed at 3 h after exercise. The 10-bout RT significantly increased gastrocnemius muscle mass in both CON and HS rats. The response of muscle hypertrophy did not differ between the groups. Therefore, MPS in response to acute RE and muscle hypertrophy in response to chronic RT were unaltered after disuse muscle atrophy.

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Section: Discussionmentioning

confidence: 99%

Response of Resistance Exercise-Induced Muscle Protein Synthesis and Skeletal Muscle Hypertrophy Are Not Enhanced After Disuse Muscle Atrophy in Rat

et al. 2020

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“…SAC opening has been connected to the activation of the Akt/mTOR pro-trophic pathway in skeletal muscle [ 217 ]. It has been recently suggested that SACs might undergo functional inactivation during unloading, possibly contributing to atrophy establishment [ 218 ]. Among SACs, the stretch-activated and Ca 2+ permeable TRPC1 channel is expressed in skeletal muscle and interacts with α-1-syntrophin PDZ domain and caveolin-3 [ 219 , 220 , 221 , 222 , 223 ].…”

Section: Master Regulators Of Muscle Atrophymentioning

confidence: 99%

Master Regulators of Muscle Atrophy: Role of Costamere Components

Gorza

Sorge

Seclì

et al. 2021

Cells

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The loss of muscle mass and force characterizes muscle atrophy in several different conditions, which share the expression of atrogenes and the activation of their transcriptional regulators. However, attempts to antagonize muscle atrophy development in different experimental contexts by targeting contributors to the atrogene pathway showed partial effects in most cases. Other master regulators might independently contribute to muscle atrophy, as suggested by our recent evidence about the co-requirement of the muscle-specific chaperone protein melusin to inhibit unloading muscle atrophy development. Furthermore, melusin and other muscle mass regulators, such as nNOS, belong to costameres, the macromolecular complexes that connect sarcolemma to myofibrils and to the extracellular matrix, in correspondence with specific sarcomeric sites. Costameres sense a mechanical load and transduce it both as lateral force and biochemical signals. Recent evidence further broadens this classic view, by revealing the crucial participation of costameres in a sarcolemmal “signaling hub” integrating mechanical and humoral stimuli, where mechanical signals are coupled with insulin and/or insulin-like growth factor stimulation to regulate muscle mass. Therefore, this review aims to enucleate available evidence concerning the early involvement of costamere components and additional putative master regulators in the development of major types of muscle atrophy.

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“…During the study of the effects of unloading on the process of mechanotransduction in skeletal muscle, an interesting phenomenon was revealed: after 24 h of unloading, an anabolic response (i.e., the rate of protein synthesis) of the isolated rat soleus muscle to a bout of eccentric contractions was significantly lower than that of the isolated muscle taken from the control animals [ 129 ]. This effect persisted at the later time-points (3 and 7 days) of unloading.…”

Section: Mechanosensory Response To the «Activity-to-disuse» Transmentioning

confidence: 99%

“…We hypothesized that unloading-induced mechano-anabolic resistance could be associated with the malfunction of the stretch-activated channels. Gadolinium treatment of the isolated rat soleus muscles taken from the intact control animals significantly reduced p70S6K phosphorylation in response to eccentric contractions [ 129 ]. At the same time, gadolinium treatment of the isolated muscles taken from the 7-day unloaded rats did not lead to a further decrease in the anabolic response to eccentric contractions [ 129 ].…”

Section: Mechanosensory Response To the «Activity-to-disuse» Transmentioning

confidence: 99%

“…Gadolinium treatment of the isolated rat soleus muscles taken from the intact control animals significantly reduced p70S6K phosphorylation in response to eccentric contractions [ 129 ]. At the same time, gadolinium treatment of the isolated muscles taken from the 7-day unloaded rats did not lead to a further decrease in the anabolic response to eccentric contractions [ 129 ]. These results indicate that the mechanisms induced by unloading are similar to those elicited by gadolinium.…”

Section: Mechanosensory Response To the «Activity-to-disuse» Transmentioning

confidence: 99%

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How Postural Muscle Senses Disuse? Early Signs and Signals

Шенкман

2020

IJMS

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A mammalian soleus muscle along with other “axial” muscles ensures the stability of the body under the Earth’s gravity. In rat experiments with hindlimb suspension, zero-gravity parabolic flights as well as in human dry immersion studies, a dramatic decrease in the electromyographic (EMG) activity of the soleus muscle has been repeatedly shown. Most of the motor units of the soleus muscle convert from a state of activity to a state of rest which is longer than under natural conditions. And the state of rest gradually converts to the state of disuse. This review addresses a number of metabolic events that characterize the earliest stage of the cessation of the soleus muscle contractile activity. One to three days of mechanical unloading are accompanied by energy-dependent dephosphorylation of AMPK, accumulation of the reactive oxygen species, as well as accumulation of resting myoplasmic calcium. In this transition period, a rapid rearrangement of the various signaling pathways occurs, which, primarily, results in a decrease in the rate of protein synthesis (primarily via inhibition of ribosomal biogenesis and activation of endogenous inhibitors of mRNA translation, such as GSK3β) and an increase in proteolysis (via upregulation of muscle-specific E3-ubiquitin ligases).

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An Anabolic Signaling Response of Rat Soleus Muscle to Eccentric Contractions Following Hindlimb Unloading: A Potential Role of Stretch-Activated Ion Channels

Cited by 23 publications

References 42 publications

Response of Resistance Exercise-Induced Muscle Protein Synthesis and Skeletal Muscle Hypertrophy Are Not Enhanced After Disuse Muscle Atrophy in Rat

Response of Resistance Exercise-Induced Muscle Protein Synthesis and Skeletal Muscle Hypertrophy Are Not Enhanced After Disuse Muscle Atrophy in Rat

Master Regulators of Muscle Atrophy: Role of Costamere Components

How Postural Muscle Senses Disuse? Early Signs and Signals

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