An anaesthetic mask for use on guineapigs (Cavia porcellus) in a stereotaxic frame: technical note

Abstract

A mask was designed to allow inhalation anaesthesia to be used on guineapigs ( Cavia porcellus) whilst placed in a stereotaxic frame. To date, anaesthesia has been maintained in approximately 60 animals using this method, with no mortalities.

The Selective 5-HT1BReceptor Inverse Agonist 1‘-Methyl-5-[[2‘-methyl-4‘- (5-methyl-1,2,4-oxadiazol-3-yl)biphenyl-4-yl]carbonyl]-2,3,6,7-tetrahydro- spiro[furo[2,3-f]indole-3,4‘-piperidine] (SB-224289) Potently Blocks Terminal 5-HT Autoreceptor Function Both in Vitro and in Vivo

“…Guinea pigs were anesthetized with 2% methoxyfluorane delivered with O 2 (1 L/min) and N 2 O (3 L/min) in an induction chamber. On attaining surgical anesthesia the guinea pigs were transferred to a stereotaxic frame which had been adapted to accommodate an anesthetic mask and scavenging unit . Anesthesia was maintained on 1−2% methoxyfluorane with O 2 (1 L/min), NO 2 (2 L/min).…”

“…On attaining surgical anesthesia the guinea pigs were transferred to a stereotaxic frame which had been adapted to accommodate an anesthetic mask and scavenging unit. 59 Anesthesia was maintained on 1-2% methoxyfluorane with O2 (1 L/min), NO2 (2 L/min). Dialysis probes were implanted into the frontal cortex (coordinates from the intra-aural zero: AP+15.0 mm, ML+2.0 mm, and 3.0 mm vertical from the dura, using the atlas of Rapisarda and Bacchelli).…”