An Analysis of Methods for the Prediction of Normal Erythrocyte Mass

McCall, Mary Sue; Reisch, Joan; Minton, Paul D.

doi:10.1093/ajcp/58.3.260

Cited by 15 publications

(8 citation statements)

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“…The amount of iron radiolabels ( 55 Fe or 59 Fe) in the total blood of the subjects was calculated from the measurements in the blood samples and estimates of total blood volume (28). These estimates take into consideration the sex, weight, and height of the individual.…”

Section: Subjectsmentioning

confidence: 99%

Iron and Zinc Absorption from Two Bean (Phaseolus vulgaris L.) Genotypes in Young Women

Donangelo

Woodhouse

King

et al. 2003

J. Agric. Food Chem.

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Extrinsic and intrinsic iron and zinc labels were used to test iron and zinc absorption from two bean (Phaseolus vulgaris) genotypes, containing normal (common beans, CB) or higher (HFeZnB) iron and zinc concentrations, fed in single meals to young women with low iron reserves. The women were divided into two groups, with one receiving a CB test meal (n = 12) and the other, an HFeZnB test meal (n = 11). The beans were intrinsically labeled hydroponically with (55)Fe (CB and HFeZnB) and with (70)Zn (HFeZnB). Concentrations of zinc and iron were 98 and 65% higher, respectively, in HFeZnB as compared to CB, but phytic acid contents were similar. Extrinsic labels were (59)Fe (CB and HFeZnB), (67)Zn (CB), and (68)Zn (HFeZnB). Iron and zinc percent absorption levels were calculated from radio-iron activity in red blood cells and from urinary excretion of zinc isotopes. Intrinsic and extrinsic iron absorption measures were highly correlated (R (2) = 0.986) (average extrinsic/intrinsic ratio was 1.00). Iron absorption was low (geometric mean < 2%) in both bean types, and total iron absorbed was not different between types. Intrinsic zinc absorption from the HFeZn beans was higher than extrinsic absorption (15.2% vs 13.4%, p < 0.05) (average extrinsic/intrinsic was 0.90). The correlation between intrinsic and extrinsic zinc measures was not as high as that for iron (R (2) = 0.719). Percent zinc absorption levels were similar in both bean types, but total extrinsic zinc absorbed was 90% higher (p < 0.05) from the HFeZnB meal. Thus, the less expensive and time-consuming extrinsic labeling may be used to screen various varieties of beans for iron bioavailability in humans, but it underestimates zinc absorption by approximately 10%. Selective breeding for high-zinc bean genotypes may improve zinc status. However, high-iron genotypes appear to have little effect on iron status when fed alone in single meals to women with low iron reserves.

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Section: Subjectsmentioning

confidence: 99%

Iron and Zinc Absorption from Two Bean (Phaseolus vulgaris L.) Genotypes in Young Women

Donangelo

Woodhouse

King

et al. 2003

J. Agric. Food Chem.

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“…It has been shown that in the extreme range of body weight, such as is found in some of our patients, blood volumes do not increase proportionally to weight. This is not the case however, when body surface is considered, as shown by Frenkel et al (1972) in 242 subjects and Retzlaff et al (1969). Furthermore in our study, treated subjects with normalized GH levels had similar vascular volumes as the controls whereas on the basis of weight, these parameters were signi¬ ficantly lower (Table 5).…”

Section: Discussionmentioning

confidence: 93%

Reversible Plasma and Red Blood Cells Volumes Increases in Acromegaly

Strauch

Therain

et al. 1977

Acta Endocrinologica

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Plasma volume (PV) and red blood cells volume (RBCV) were measured in 76 normotensive acromegalic patients (42 males and 34 females) and compared with those of 28 controls on the basis of body surface. In untreated males PV and RBCV were significantly higher than in normals (P <0.001) and total blood volume (TBV) was above the normal range in 85 %. Untreated females also showed an elevated PV but had significantly lower RBCV than the male patients. The only vascular volume of the patients which correlated significantly with the body weight or surface was RBCV in females.One to five studies were done after treatment in 52 patients and analyzed as a function of plasma GH levels. Plasma volume reverted to the normal range (males) or was significantly lower than in untreated patients (females) in the groups of patients with normal GH levels while RBCV normalized at higher GH concentrations. At similar GH levels, treated patients without pituitary failure (n = 35) or given T4 and/or cortisol substitution for hypopituitarism showed similar vascular volumes. The plasma volume and TBV in both sexes and RBCV in males was significantly correlated with Log 10 GH levels. It is concluded from these data and other experimental evidence that endogenous GH hypersecretion is responsible for the reversible PV and RBCV increases frequently found in acromegalic patients.

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“…Whole blood was drawn from the anterior vena cava prior to oral and cecal stable isotope dosing and 14 days thereafter for hemoglobin and stable isotope ratio analyses. Inductively coupled plasma mass spectrometry (ICP-MS) with an Agilent 7500CS analyzer mass spectrometer was used to analyze the amount of Fe stable isotope ( 58 Fe or 54 Fe) in excess of the naturally occurring amount in the total circulating Hb of the animals, thereby providing a measure of Fe absorption (28 ).…”

Section: Methodsmentioning

confidence: 99%

Dietary Inulin Supplementation Does Not Promote Colonic Iron Absorption in a Porcine Model

Patterson

Rutzke

Fubini³

et al. 2009

J. Agric. Food Chem.

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Prebiotics may enhance iron bioavailability by increasing iron absorption in the colon. Anemic pigs fitted with cecal cannulas were fed a low-iron diet with or without 4% inulin. Over 7 days, pigs were administered 1 mg of (54)Fe in the morning feed followed by cannula infusion of 0.5 mg of (58)Fe to measure total and colonic iron absorption, respectively. Whole blood was drawn prior to the initial dosing and 14 days thereafter for hemoglobin concentration and stable isotope ratio analyses. The prebiotic role of inulin was confirmed by increases in lactobacilli and bifidobacteria with reductions in clostridia using terminal restriction fragment length polymorphism (TRFLP). Total iron absorption was 23.2 +/- 2.7 and 20.7 +/- 3.5% (mean +/- SEM; p > 0.05), while colonic iron absorption was 0.4 +/- 0.1 and 1.0 +/- 0.2% (mean +/- SEM; p > 0.05) in inulin-fed and control pigs, respectively. These results show that the colon does not make a significant contribution to total iron absorption in iron-deficient pigs and that inulin does not affect iron absorption in the colon.

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An Analysis of Methods for the Prediction of Normal Erythrocyte Mass

Cited by 15 publications

References 0 publications

Iron and Zinc Absorption from Two Bean (Phaseolus vulgaris L.) Genotypes in Young Women

Iron and Zinc Absorption from Two Bean (Phaseolus vulgaris L.) Genotypes in Young Women

Reversible Plasma and Red Blood Cells Volumes Increases in Acromegaly

Dietary Inulin Supplementation Does Not Promote Colonic Iron Absorption in a Porcine Model

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