An antenna-biased carboxylesterase is specifically active to plant volatiles in Spodoptera exigua

He, Pinjing; Zhang, Yanan; Yang, Ke; Li, Zhaoqun; Dong, Shuang-Lin

doi:10.1016/j.pestbp.2015.03.009

Cited by 47 publications

(49 citation statements)

References 50 publications

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“…In this clade, CsupCXE10 fell into a branch together with two well-characterized ODEs: SlitCXE10 and SexiCXE10 ( Durand et al. 2010b ; He et al. 2015 ), suggested these CXEs are orthologues and may have similar functions in odorant degradation.…”

Section: Resultsmentioning

confidence: 95%

Identification of Putative Carboxylesterase and GlutathioneS-transferase Genes from the Antennae of theChilo suppressalis(Lepidoptera: Pyralidae)

et al. 2015

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In insects, rapid degradation of odorants in antennae is extremely important for the sensitivity of olfactory receptor neurons. Odorant degradation in insect antennae is mediated by multiple enzymes, especially the carboxylesterases (CXEs) and glutathione S-transferases (GSTs). The Asiatic rice borer, Chilo suppressalis, is an economically important lepidopteran pest which causes great economic damage to cultivated rice crops in many Asian countries. In this study, we identified 19 putative CXE and 16 GST genes by analyzing previously constructed antennal transcriptomes of C. suppressalis. BLASTX best hit results showed that these genes are most homologous to their respective orthologs in other lepidopteran species. Phylogenetic analyses revealed that these CXE and GST genes were clustered into various clades. Reverse-transcription quantitative polymerase chain reaction assays showed that three CXE genes (CsupCXE8, CsupCXE13, and CsupCXE18) are antennae-enriched. These genes are candidates for involvement in odorant degradation. Unexpectedly, none of the GST genes were found to be antennae-specific. Our results pave the way for future researches of the odorant degradation mechanism of C. suppressalis at the molecular level.

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Section: Resultsmentioning

confidence: 95%

Identification of Putative Carboxylesterase and GlutathioneS-transferase Genes from the Antennae of theChilo suppressalis(Lepidoptera: Pyralidae)

et al. 2015

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“…The general theory of the insect olfactory process is that environmental odorants penetrate the pores of sensilla into sensilla lymph, where odorant‐binding proteins (OBPs) act as carriers that bind and transport odorants to odorant receptors (ORs) or ionotropic receptors (IRs) located on olfactory sensory neurons (OSNs) . The odorants are then degraded by various odorant degrading enzymes to maintain the sensitivity of the OSNs . The chemical signals are then transformed to electrical signals to trigger behavioral responses in the insect …”

Section: Introductionmentioning

confidence: 99%

Evolution and functional analysis of odorant‐binding proteins in three rice planthoppers: Nilaparvata lugens, Sogatella furcifera, and Laodelphax striatellus

Chen

et al. 2019

Pest Management Science

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BACKGROUND The white‐backed planthopper (WBPH) Sogatella furcifera, the brown planthopper (BPH) Nilaparvata lugens, and the small brown planthopper (SBPH) Laodelphax striatellus are three notorious rice pests that cause annual losses in rice yield through sap‐sucking and virus transmission. Odorant‐binding proteins (OBPs) are crucial olfactory genes involved in host‐seeking behavior. RESULTS We discovered the presence of 12, 12, and 16 OBPs in WBPH, BPH, and SBPH, respectively, including two novel OBPs in BPH and seven novel OBPs in SBPH. Phylogenetic analysis indicated that most of these OBPs have homologous genes, and one group (SfurOBP11, NlugOBP8, and LstrOBP2) show a slower evolution rate and are more conserved. Further, in vitro binding studies demonstrated that the three OBPs have similar binding affinities for some rice plant volatiles. Finally, RNA interference (RNAi) successfully inhibited the mRNA expression of the three OBPs, and in vivo behavioral tests showed that the OBP‐deficient rice planthoppers were partly anosmic and lost some of their ability to locate rice plants. CONCLUSION These results demonstrate the crucial role of the rice planthopper OBP genes in seeking rice plants. This information complements the current genetic resources for the development of RNAi‐based transgenic rice and other pest management technologies. © 2018 Society of Chemical Industry

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“…(1) penetration of odorants into sensillar lymph through pores, where odorant binding proteins (OBPs)/chemosensory proteins (CSPs) recognize and bind them; (2) OBP/CSP-mediated transfer of odorants through sensillar lymph to two families of integral membrane proteins, odorant receptors (ORs) and ionotropic receptors (IRs), which are located on dendrites of olfactory receptor neurons (ORNs) (He et al, 2015;Jacquin-Joly and Maïbèche-Coisne, 2009;Leal, 2013;S. Liu et al, 2014;Pelosi et al, 2014;Vogt, 2005;Wang et al, 2016;Zhou et al, 2015;Zhu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Molecular identification and sex distribution of two chemosensory receptor families in Athetis lepigone by antennal transcriptome analysis

Zhang

Sun

et al. 2016

Journal of Asia-Pacific Entomology

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An antenna-biased carboxylesterase is specifically active to plant volatiles in Spodoptera exigua

Cited by 47 publications

References 50 publications

Identification of Putative Carboxylesterase and GlutathioneS-transferase Genes from the Antennae of theChilo suppressalis(Lepidoptera: Pyralidae)

Identification of Putative Carboxylesterase and GlutathioneS-transferase Genes from the Antennae of theChilo suppressalis(Lepidoptera: Pyralidae)

Evolution and functional analysis of odorant‐binding proteins in three rice planthoppers: Nilaparvata lugens, Sogatella furcifera, and Laodelphax striatellus

Molecular identification and sex distribution of two chemosensory receptor families in Athetis lepigone by antennal transcriptome analysis

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