An anticarbohydrate monoclonal antibody inhibits cell-cell adhesion in many species of Dictyostelium but not of Polysphondylium

Springer, R. W.; Ahern, Julie A.

doi:10.1016/0014-4827(90)90296-m

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…For example, glycosylation of contact site A, the most extensively studied cell adhesion molecule in D. discoideum, had been reported by several groups to be important for its function (16,29,40) but was reported by other groups as not so important (9,11,23,27). However, in other species of slime moulds, surface carbohydrates was shown indeed to be essential for cell adhesion (29,30,38). Therefore, it would be interesting to reveal the roles of carbohydrates in cell interactions during the sexual development of D. discoideum.…”

Section: Discussionmentioning

confidence: 99%

Involvement of Cell Surface Carbohydrates in the Sexual Cell Fusion of Dictyostelium discoideum

1991

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In order to analyze the molecular mechanism of sexual cell fusion between cells of HM1 and NC4 (opposite mating type strains in Dictyostelium discoideum), monoclonal antibodies were raised against partially-purified gp 70, a fusion-related protein of HM1 cells. The antibodies were screened for activity to inhibit cell fusion and 9 hybridoma clones were obtained. One of the fusion-blocking monoclonal antibodies, mAblG7, was used for further analysis. It recognized nearly ten bands in an immunoblot of fusion competent HM1 cells, but no bands when HM1 membrane proteins had been deglycosylated. These results suggest the importance of carbohydrates in the cell fusion process. To confirm this possibility, effects of sugars or lectins on cell fusion were examined. Although inhibition by the sugars was incomplete, Con A, WGA, LCA, strongly inhibited cell fusion. Furthermore, tunicamycin inhibited the acquisition of fusion competence in HMI cells, indicating the importance of N-linked glycosylation of proteins in cell fusion. All above results suggest that N-linked carbohydrates on HM1 cell surface are involved in the sexual cell fusion of D. discoideum.

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Section: Discussionmentioning

confidence: 99%

Involvement of Cell Surface Carbohydrates in the Sexual Cell Fusion of Dictyostelium discoideum

1991

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Antibodies specific for gp40 inhibit cell‐cell adhesion by cross‐linking the protein on the surface of Dictyostelium purpureum

Springer

Haywood-Reid

1993

J of Cellular Biochemistry

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We have previously suggested a role for gp40 in cell-cell adhesion in Dictyostelium purpureum from the fact that antibodies specific for this protein inhibited adhesion in an in vitro assay [Springer: Dev Biol 133:447-455, 1989]. To further confirm the role mutants lacking the protein were isolated and characterized. To our surprise, the mutants had normal adhesive properties both in vitro and in situ. These results lead us to the conclusion that gp40 is not necessary for the cell-cell adhesions observed and may not be a molecule which directly participates in these adhesions. When studied further, we found that adhesion-inhibitory antibodies were only effective as divalent IgG. Monovalent Fab fragments of the same antibodies could not inhibit adhesion. The inhibitory antibodies also caused the cells to remain rounded and incapable of attaching to plastic surfaces. We conclude that when divalent antibodies specific for gp40 cross-link this protein on the cell surface a cytoskeletal change prevents them from attaching to substratum as well as to other cells, thereby inhibiting cell-cell adhesion. We suggest that an alternative mechanism for inhibition of cell-cell adhesion by divalent antibodies exists and should be considered before proposing a direct role for a protein in adhesion.

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The Role of Carbohydrates in Dictyostelium discoideum Cohesion.

Springer

直人²

1991

Trends in Glycoscience and Glycotechnology

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An anticarbohydrate monoclonal antibody inhibits cell-cell adhesion in many species of Dictyostelium but not of Polysphondylium

Cited by 3 publications

References 20 publications

Involvement of Cell Surface Carbohydrates in the Sexual Cell Fusion of Dictyostelium discoideum

Involvement of Cell Surface Carbohydrates in the Sexual Cell Fusion of Dictyostelium discoideum

Antibodies specific for gp40 inhibit cell‐cell adhesion by cross‐linking the protein on the surface of Dictyostelium purpureum

The Role of Carbohydrates in Dictyostelium discoideum Cohesion.

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