An antifungal peptide from passion fruit (Passiflora edulis) seeds with similarities to 2S albumin proteins

Pelegrini, Patrícia B.; Noronha, Eliane Ferreira; Muñiz, Manuel; Vasconcelos, Ilka M.; Chiarello, Marileusa D.; Oliveira, José T.A.; Franco, Octávio L.

doi:10.1016/j.bbapap.2006.04.010

Cited by 74 publications

(46 citation statements)

References 51 publications

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“…Phytopathogenic bacteria cause enormous problems in agriculture, resulting in severe economic losses, since plants are the main nutrient sources of these pathogens. 23 Ralstonia solanacearum and Xanthomonas axonopodis are the most extensively studied phytopathogens in potato (Solanum tuberosum), tobacco (Nicotiana tabacum), and tomato (Lycopersicon esculentum) plant systems. The increasing population of these phytopathogens causes degradation of the occluded xylem vessels and the death of the plants.…”

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confidence: 99%

Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects

Aravinthan

Govarthanan

Selvam³

et al. 2015

IJN

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A rapid, green phytosynthesis of silver nanoparticles (AgNPs) using the aqueous extract of Helianthus tuberosus (sunroot tuber) was reported in this study. The morphology of the AgNPs was determined by transmission electron microscopy (TEM). Scanning electron microscopy–energy-dispersive spectroscopy (SEM–EDS) and X-ray powder diffraction (XRD) analysis confirmed the presence of AgNPs. Fourier transform infrared spectroscopy (FTIR) analysis revealed that biomolecules in the tuber extract were involved in the reduction and capping of AgNPs. The energy-dispersive spectroscopy (EDS) analysis of the AgNPs, using an energy range of 2–4 keV, confirmed the presence of elemental silver without any contamination. Further, the synthesized AgNPs were evaluated against phytopathogens such as Ralstonia solanacearum and Xanthomonas axonopodis . The AgNPs (1–4 mM) extensively reduced the growth rate of the phytopathogens. In addition, the cytotoxic effect of the synthesized AgNPs was analyzed using rat splenocytes. The cell viability was decreased according to the increasing concentration of AgNPs and 67% of cell death was observed at 100 μg/mL.

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mentioning

confidence: 99%

Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects

Aravinthan

Govarthanan

Selvam³

et al. 2015

IJN

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“…From seeds of guava (Psidium guajava) and passion fruit (Passiflora edulis), the antifungal and antibacterial peptides Pg-AMP1, passiflin and a 2S albumin-like were isolated. PgAMP1 comprises approximately 6 KDa of molecular mass and small amounts of a homodimer; amino acid sequencing indicated it belongs to glycine-rich plant protein family, being the first one having activity towards Gram-negative bacteria; instead passiflin and the 2S albumin-like peptide show high antifungal properties (Pelegrini et al, 2006;Lam & Ng, 2009). Several plant species from Atlantic rainforest and "cerrado" biomes have been studied to confirm and explain their antimicrobial activity as transmitted popular medicine (reviewed in Pestana- .…”

Section: Isolationmentioning

confidence: 99%

In Silico Identification of Plant-Derived Antimicrobial Peptides

Pestana-Calsa¹,

Calsa²

2011

Systems and Computational Biology - Molecular and Cellular Experimental Systems

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“…In the case of plant tissues, AMPs have also been isolated from flowers, leaves, roots and seeds, among others [5][6][7][8]. Recently, three antimicrobial peptides were isolated from green coconut water (Cn-AMPs), in a series called Cn-AMP1 (SVAGRAQGM), Cn-AMP2 (TESYFVFSVGM) and Cn-AMP3 (YCSYTMEA) [9].…”

Section: Introductionmentioning

confidence: 99%

Structural insights into Cn‐AMP1, a short disulfide‐free multifunctional peptide from green coconut water

et al. 2015

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a b s t r a c tMultifunctional and promiscuous antimicrobial peptides (AMPs) can be used as an efficient strategy to control pathogens. However, little is known about the structural properties of plant promiscuous AMPs without disulfide bonds. CD and NMR were used to elucidate the structure of the promiscuous peptide Cn-AMP1, a disulfide-free peptide isolated from green coconut water. Data here reported shows that peptide structure is transitory and could be different according to the microenvironment. In this regard, Cn-AMP1 showed a random coil in a water environment and an a-helical structure in the presence of SDS-d 25 micelles. Moreover, deuterium exchange experiments showed that Gly4, Arg5 and Met9 residues are less accessible to solvent, suggesting that flexibility and cationic charges seem to be essential for Cn-AMP1 multiple activities.

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An antifungal peptide from passion fruit (Passiflora edulis) seeds with similarities to 2S albumin proteins

Cited by 74 publications

References 51 publications

Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects

Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects

In Silico Identification of Plant-Derived Antimicrobial Peptides

Structural insights into Cn‐AMP1, a short disulfide‐free multifunctional peptide from green coconut water

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