An antimony-phosphomolybdate microassay of ATPase activity through the detection of inorganic phosphate

Discovery of an ʟ-amino acid ligase implicated in Staphylococcal sulfur amino acid metabolism

“…First, a colorimetric assay of phosphate was used to determine if SAOUHSC_02373 possessed LAL activity ( 15 , 16 ). Initially, activity was measured for a reaction containing all 20 standard ʟ-amino acids as substrates ( Fig.…”

“…For determination of WT LdmS kinetic parameters, a pyruvate kinase/lactate dehydrogenase coupled-assay was used as previously described ( 16 ). Reactions were performed at 37 °C in 96-well half-area plates (Corning Ref.…”

Colorimetry‐Based Phosphate Measurement for Polymerase Elongation

“…During the elongation reaction, PPi was released upon base extension. It is digested by PPase to become Pi, and the resultant Pi can be measured by methods including Mo-Sb colorimetry and BIOMOL® Green (Figure 3(c)) [18][19][20][22][23][24].…”

“…The Mo-Sb method detects Pi, but not PPi, in solutions [18][19][20]. PPi is produced during strand elongation.…”

“…Pi detection is widely used in chemical, environmental, and biological research. The Mo-Sb colorimetric method can be used for Pi detection and is mostly used with soil or other environmental samples [18][19][20]. BIOMOL® Green is a commercial reagent developed from Malachite Green reagent [21] and has been used successfully in the study of proteins having ATPase activity [22][23][24].…”