An Antimutagenic Metabolite, Streptovaricin C, Isolated from Streptomyces sp.

Ooka, Kazuhiro; Tanaka, Michiyasu; Ishikawa, Tsutomu; Kato, Fumio

doi:10.1248/bpb.22.107

Cited by 4 publications

(2 citation statements)

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“…1985). And the molecular ion of m/z 770 [M+H] + and 792 [M+Na] + was possible related to ansamycin–streptovaricin C production in orange colour fraction (Ooka et al. 1999) of 3‐27 strain metabolites (Fig.…”

Section: Resultsmentioning

confidence: 99%

A new method for rapid identification of ansamycin compounds by inactivating KLM gene clusters in potential ansamycin-producing actinomyces

Wang

Zhang

Sun

et al. 2012

Journal of Applied Microbiology

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Aims: In this study, we explored the possibility of construction of a ‘universal targeting vector’ by Red/ET recombination to inactivate L gene encoding 3‐amino‐5‐hydroxybenzoic acid (AHBA)‐oxidoreductase in AHBA biosynthetic gene cluster to facilitate the detection of ansamycins production in actinomycetes. Methods and Results: Based on the conserved regions of linked AHBA synthase (K), oxidoreductase (L) and phosphatase (M) gene clusters, degenerate primers were designed and PCR was performed to detect KLM gene clusters within 33 AHBA synthase gene‐positive actinomycetes strains. Among them, 22 KLM gene cluster‐positive strains were identified. A ‘universal targeting vector’ was further constructed using the 50‐nt homologous sequences chosen from four strains internal L gene in KLM gene clusters through Red/ET recombination. The L gene from nine of the KLM gene cluster‐positive actinomycetes strains was inactivated by insertion of a kanamycin (Km) resistance marker into its internal region from the ‘universal targeting vector’. By comparison of the metabolites produced in parent strains with those in L gene‐inactivated mutants, we demonstrated the possible ansamycins production produced by these strains. One strain (4089) was proved to be a geldanamycin producer. Three strains (3‐20, 7‐32 and 8‐32) were identified as potential triene‐ansamycins producers. Another strain (3‐27) was possible to be a streptovaricin C producer. Strains 24‐100 and 4‐124 might be served as ansamitocin‐like producers. Conclusions: The results confirmed the feasibility that a ‘universal targeting vector’ could be constructed through Red/ET recombination using the conserved regions of KLM gene clusters to detect ansamycins production in actinomycetes. Significance and Impact of the Study: The ‘universal targeting vector’ provides a rapid approach in certain degree to detect the potential ansamycin producers from the 22 KLM gene cluster‐positive actinomycetes strains.

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Section: Resultsmentioning

confidence: 99%

A new method for rapid identification of ansamycin compounds by inactivating KLM gene clusters in potential ansamycin-producing actinomyces

Wang

Zhang

Sun

et al. 2012

Journal of Applied Microbiology

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“…125 Streptovaricin C 324 has been obtained from the soil micro-organism Streptomyces species KMl-30. 126 Depsipeptide elastase inhibitors YH-47141 325 and YM-4742 326 contain the interesting hydrated vicinal tricarbonyl grouping. Their synthesis required creation of the tricarbonyl system in protected form.…”

Section: Peptide Alkaloidsmentioning

confidence: 99%

Amaryllidaceae, Sceletium, imidazole, oxazole, thiazole, peptide and miscellaneous alkaloids (July 1998 to June 1999)

Lewis

2001

Nat. Prod. Rep.

102

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Amaryllidaceae, Sceletium, imidazole, oxazole, thiazole, peptide and miscellaneous alkaloids

Lewis

2002

Nat. Prod. Rep.

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This review covers alkaloids classified in the plant families Amaryallidaceae and Sceletium. In addition alkaloids possessing imidazole, oxazole and thiazole structures are listed followed by alkaloids possessing peptide linkages. Finally miscellaneous alkaloids include those compounds found naturally which cannot be classified into known groups; a relative Pandora's box. A total of 620 structures has been reviewed from 218 references found in the literature for the period July 1999 to June 2000.

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An Antimutagenic Metabolite, Streptovaricin C, Isolated from Streptomyces sp.

Cited by 4 publications

References 1 publication

A new method for rapid identification of ansamycin compounds by inactivating KLM gene clusters in potential ansamycin-producing actinomyces

A new method for rapid identification of ansamycin compounds by inactivating KLM gene clusters in potential ansamycin-producing actinomyces

Amaryllidaceae, Sceletium, imidazole, oxazole, thiazole, peptide and miscellaneous alkaloids (July 1998 to June 1999)

Amaryllidaceae, Sceletium, imidazole, oxazole, thiazole, peptide and miscellaneous alkaloids

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