An apparent halt to the decline ofSimulium woodiin the Usambara foci of onchocerciasis in Tanzania

Kalinga, Akili; Post, R.J.

doi:10.1179/136485911x12899838683403

Cited by 5 publications

(3 citation statements)

References 3 publications

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“…This period also saw a reduction in the percentage of mature S. woodi infected with the disease-causing agent, the nematode Onchocerca volvulus, from 17% to 3% (Muro and Raybould, 1990). While this decline plateaued, 25 years later populations had not recovered, suggesting deforestation in the area had a significant and long-term impact on vector numbers (Kalinga and Post, 2011).…”

Section: Physical Changes To the Environmentmentioning

confidence: 99%

Epidemiology of the zoonotic malaria Plasmodium knowlesi in changing landscapes

Cuenca

Key

Jumail

et al. 2021

Current Research on Naturally Transmitted Plasmodium Knowlesi

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Section: Physical Changes To the Environmentmentioning

confidence: 99%

Epidemiology of the zoonotic malaria Plasmodium knowlesi in changing landscapes

Cuenca

Key

Jumail

et al. 2021

Current Research on Naturally Transmitted Plasmodium Knowlesi

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“…This was the area where the savannah strain of parasite causing blindness [5,6] are residing. On the other hand, another strain, the forest type non blinding was responsible for the severe skin disease, commonly found in Central & East Africa [7,8].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Clinical, Serological and Parasitological Aspects of Onchocercal Skin Disease, 20 Years of Community Directed Treatment with Ivermectin (CDTI) in the Ruvuma, Southwestern Tanzania

Makunde

2021

AJBSR

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“…Molecular methods such as polymerase chain reaction (PCR) and real-time PCR have been developed for detection of S. aureus and MRSA. [8][9][10] These methods can be used to analyze clinical specimens (sputum, blood and bronchoalveolar lavage fluid (BALF)) with high specificity and sensitivity. However, the simplicity and rapidity of these methods are relatively limited, due to its long genomic template extraction and amplification protocols.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticles-Based Biosensor Coupled with Multiplex Loop-Mediated Isothermal Amplification for Detection of Staphylococcus aureus and Identification of Methicillin-Resistant S. aureus

Lei

et al. 2020

IDR

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Introduction: Staphylococcus aureus (S. aureus), including methicillin-resistant S. aureus (MRSA), is a common human pathogen, which can cause a variety of infections from mild to severe. In this article, a new diagnostic method called multiplex loop-mediated isothermal amplification combined with nanoparticles-based lateral flow biosensor (mLAMP-LFB) has been developed, which was proved to be fast, reliable, and simple for detecting S. aureus, and differentiate MRSA from methicillin-susceptible S. aureus (MSSA). Materials and Methods: We designed a set of six primers targeting the nuc gene of S. aureus, and a set of five primers targeting the mecA gene of MRSA. The lateral flow biosensor visually reported the S. aureus-LAMP results within 2 mins. S. aureus species and non-S. aureus species were used to identify the specificity and sensitivity of the assay. Results: The best conditions for LAMP were 50 mins at 63°C, and the sensitivity was 100 fg. No cross-reactivity was shown and the specificity of this assay is 100%. This assay requires 20 mins for DNA preparation, 50 mins for isothermal amplification and 2 mins for biosensor detection. The total time is within 75 mins. Among 96 sputum samples, LAMP-LFB and traditional culture method showed the same results, 8 (8.33%) samples were MRSA-positive, and 9 (9.38%) samples were MSSA-positive. Seven (7.29%) samples were MRSA-positive and 7 (7.29%) were MSSA-positive by PCR method. Compared with the culture method, diagnostic accuracy of m-LAMP-LFB assay was 100%. The results showed that the m-LAMP-LFB method has better detection ability than the PCR method. Discussion: In short, this m-LAMP-LFB assay is a specific and sensitive method that can quickly identify S. aureus stains, and distinguish MRSA from MSSA, and can be used as a new molecular method for detection of S. aureus in laboratories.

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An apparent halt to the decline ofSimulium woodiin the Usambara foci of onchocerciasis in Tanzania

Cited by 5 publications

References 3 publications

Epidemiology of the zoonotic malaria Plasmodium knowlesi in changing landscapes

Epidemiology of the zoonotic malaria Plasmodium knowlesi in changing landscapes

Assessment of the Clinical, Serological and Parasitological Aspects of Onchocercal Skin Disease, 20 Years of Community Directed Treatment with Ivermectin (CDTI) in the Ruvuma, Southwestern Tanzania

<p>Nanoparticles-Based Biosensor Coupled with Multiplex Loop-Mediated Isothermal Amplification for Detection of <em>Staphylococcus aureus</em> and Identification of Methicillin-Resistant <em>S. aureus</em></p>

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