An aptamer based surface plasmon resonance biosensor for the detection of ochratoxin A in wine and peanut oil

Zhu, Zhiling; Feng, Mengxue; Zuo, Li; Zhu, Zhentai; Wang, Fengwei; Long, Chen; Li, Jinghua; Shan, Guangzhi; Luo, Shi-Zhong

doi:10.1016/j.bios.2014.10.059

Cited by 141 publications

(57 citation statements)

References 35 publications

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“…In comparison with traditional detection methods, like X-ray, HPLC, and HPLC-MS, SPR technology can perform kinetics parameters (association and dissociation process) measurement, concentration measurement, and real-time molecule detection. It has been widely used in chemical, biology, agriculture, environment, and food safety [6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

Zhang

Settu

Liu

2018

Advances in Condensed Matter Physics

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Surface plasmon resonance (SPR) is a real-time, label-free, and high-sensitive detection technology. SPR has been widely used in many applications such as biomolecular interaction analysis, environmental monitoring, and medical diagnostics. However, conventional SPR sensor systems usually require expensive equipment and complicated optics. In this paper, we have demonstrated a rapid prototyping of angle-scanning SPR for bioanalytical investigation. Rapid prototyping was attained by utilizing the FDM (fused deposition modeling) based 3D (three-dimensional) printing technology. Two rotating platforms were employed to drive the laser source and photodiode, respectively. A temperature regulation unit was incorporated to maintain the system temperature in order to reduce the temperature effect. The proposed SPR rapid prototyping yielded a refractive index resolution of 6.4×10 −6 RIU (refractive index unit), and the biotin-avidin system validated the kinetics parameters measurement capability. The obtained results indicated that the FDM 3D printing has great potential for developing rapid-prototyping SPR system.

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Section: Introductionmentioning

confidence: 99%

Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

Zhang

Settu

Liu

2018

Advances in Condensed Matter Physics

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“…benzoylecgonine, seafood toxin-tetrodotoxin, ochratoxin, and etc. [31][32][33]. Park et al [34] reported a direct detection method of AFB1 by using a SPR immunobiosensor.…”

Section: Introductionmentioning

confidence: 99%

Aptamer based surface plasmon resonance sensor for aflatoxin B1

Sun

Zhao

2017

Microchim Acta

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The authors describe a surface plasmon resonance (SPR) based aptasensor for the carcinogenic mycotoxin aflatoxin B1 (AFB1) in a direct assay format. The aptamer is immobilized on the surface of a commercial sensor chip, and the SPR signal increases on binding of AFB1. The sensor chip can be fully regenerated by passing a flow of buffer over it upon which bound AFB1 dissociates from the aptamer. The biosensor works in the 0.4 nM to 200 nM AFB1 concentration range and has a 0.4 nM detection limit. It allows AFB1 to be determined in complex samples such as diluted red wine and beer. The assay is sensitive, and the chip is easily regenerated and stable. The method therefore overcomes certain limitations of antibody-based SPR assays and of competitive SPR assays for AFB1.

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“…OTA can occur in a broad range of food matrices, such as grape juice, cereals, wine, barely, milk, and meat products [3][4][5]. OTA is hepatotoxic, nephrotoxic, teratogenic, immunotoxic, and mutagenic to humans and animals [6,7].…”

Section: Introductionmentioning

confidence: 99%

A fluorescent aptasensor based on a DNA pyramid nanostructure for ultrasensitive detection of ochratoxin A

et al. 2016

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Analytical techniques for detection of ochratoxin A (OTA) in food products and blood serum are of great significance. In this study, a fluorescent aptasensor was developed for sensitive and specific detection of OTA, based on a DNA pyramid nanostructure (DPN) and PicoGreen (PG) dye. The designed aptasensor inherits characteristics of DPN, such as high stability and capacity for PG loading. PG, as a fluorescent dye, could bind to double-stranded DNA (dsDNA). In the absence of OTA, the pyramid structure of DPN remains intact, leading to a very strong fluorescence emission. Because of higher affinity of aptamer for its target relative to its complementary strand, upon addition of target, the pyramid structure of DPN is disassembled, leading to a weak fluorescence emission. The presented aptasensor showed high specificity toward OTA with a limit of detection (LOD) as low as 0.135 nM. Besides, the designed sensing strategy was successfully utilized to recognize OTA in serum and grape juice with LODs of 0.184 and 0.149 nM, respectively.

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An aptamer based surface plasmon resonance biosensor for the detection of ochratoxin A in wine and peanut oil

Cited by 141 publications

References 35 publications

Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

Aptamer based surface plasmon resonance sensor for aflatoxin B1

A fluorescent aptasensor based on a DNA pyramid nanostructure for ultrasensitive detection of ochratoxin A

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