An ascorbic acid-enriched tomato genotype to fight UVA-induced oxidative stress in normal human keratinocytes

Petruk, Ganna; Raiola, Assunta; Giudice, Rita Del; Barone, Amalia; Frusciante, Luigi; Rigano, Maria Manuela; Monti, Daria Maria

doi:10.1016/j.jphotobiol.2016.08.047

Cited by 49 publications

(44 citation statements)

References 31 publications

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“…In addition, UVB irradiation also induces ROS formation which then causes oxidative damage to the skin cells [8,70]. Natural as well as synthetic compounds having the ability to reduce excessive free radicals and prevent or reduce DNA damage could potentially be exploited for skin photoprotection [17,50,[71][72][73][74]. Recently, 1,25(OH) 2 D 3 has been shown to protect the skin against oxidative stress [34][35][36] and to induce DNA damage/repair process [36][37][38]48] as well as activating Nrf2 expression [34,39].…”

Section: Discussionmentioning

confidence: 99%

Protective effects of novel derivatives of vitamin D3 and lumisterol against UVB-induced damage in human keratinocytes involve activation of Nrf2 and P53 defense mechanisms

Chaiprasongsuk

Kim

Holick

et al. 2018

Free Radical Biology and Medicine

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We tested whether novel CYP11A1-derived vitamin D 3-and lumisterol-hydroxyderivatives, including 1,25(OH) 2 D 3 , 20(OH)D 3 , 1,20(OH) 2 D 3 , 20,23(OH) 2 D 3 , 1,20,23(OH) 3 D 3 , lumisterol, 20(OH)L 3 , 22(OH)L 3 , 20,22(OH) 2 L 3 , and 24(OH)L 3 , can protect against UVB-induced damage in human epidermal keratinocytes. Cells were treated with above compounds for 24 h, then subjected to UVB irradiation at UVB doses of 25, 50, 75, or 200 mJ/cm 2 , and then examined for oxidant formation, proliferation, DNA damage, and the expression of genes at the mRNA and protein levels. Oxidant formation and proliferation were determined by the DCFA-DA and MTS assays, respectively. DNA damage was assessed using the comet assay. Expression of antioxidative genes was evaluated by real-time RT-PCR analysis. Nuclear expression of CPD, phospho-p53, and Nrf2 as well as its target proteins including HO-1, CAT, and MnSOD, were assayed by immunofluorescence and western blotting. Treatment of cells with the above compounds at concentrations of 1 or 100 nM showed a dose-dependent reduction in oxidant formation. At 100 nM they inhibited the proliferation of cultured keratinocytes. When keratinocytes were irradiated with 50-200 mJ/cm 2 of UVB they also protected against DNA damage, and/or induced DNA repair by enhancing the repair of 6-4PP and attenuating CPD levels and the tail moment of comets. Treatment with test compounds increased expression of Nrf2-target genes involved in the antioxidant response including GR, HO-1, CAT, SOD1, and SOD2, with increased protein expression for HO-1, CAT, and MnSOD. The treatment also stimulated the phosphorylation of p53 at Ser-15, increased its concentration in the nucleus and enhanced Nrf2 translocation into the nucleus. In conclusion, pretreatment of keratinocytes with 1,25(OH) 2 D 3 or CYP11A1-derived vitamin D 3-or lumisterol hydroxy-derivatives, protected them against UVB-induced damage via activation of the Nrf2-dependent antioxidant response and p53-phosphorylation, as well as by the induction of the DNA repair system. Thus, the new vitamin D 3 and lumisterol hydroxy-derivatives represent promising anti-photodamaging agents.

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Section: Discussionmentioning

confidence: 99%

Protective effects of novel derivatives of vitamin D3 and lumisterol against UVB-induced damage in human keratinocytes involve activation of Nrf2 and P53 defense mechanisms

Chaiprasongsuk

Kim

Holick

et al. 2018

Free Radical Biology and Medicine

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“…In the first one, DNA can directly absorb energy from UV irradiation that, if it exceeds the capacity of DNA, can result in the dimerisation of two adjacent pyrimidine bases and destroy the original native DNA structure; in the other type, free radicals/ROS such as hydroxyl free radicals, superoxide anions, oxygen free radicals, and their active precursor singlet oxygen, hydrogen peroxide and ozone, which are generated during oxidative respiration and exposure to UV irradiation, are strongly oxidising and can attack the DNA skeleton on the DNA of deoxyribose by generating substrate free radicals or hydrogenation reaction, and the secondary reaction of free radicals can cause serious damage to the structure of DNA. [6][7][8]31,43] Sunlight can produce a strong UV radiation, and it can be difficult for humans to avoid UV radiation, especially for people who work outdoors for extended periods, and consequently the DNA in their skin is under threat from UV-induced damage. Some of the sunscreen products contain chemical compounds that can cause skin allergies and other adverse reactions, and prolonged usage can lead to skin diseases.…”

Section: Discussionmentioning

confidence: 99%

“…[4,5] UV radiation is among the most harmful exogenous factors for human skin due to its ability to induce free radical/ROS production. [6][7][8] Abundant free radicals/ROS in the skin mainly result from solar UV and cause long-term radiation of the skin. [9] Free radicals/ROS cause immense damage to DNA [10,11] , resulting in various biological effects on the skin, such as generation of the pigment AD cool-headed, skin ageing, skin wrinkling, immune suppression, and even skin cancer [12] , which is a major health hazard worldwide.…”

Section: Introductionmentioning

confidence: 99%

DNA protection activity of a hydroethanol extract and six polyphenol monomers fromMorus alba L. (mulberry) twig

Jia

et al. 2017

International Journal of Food Properties

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Morus alba L. (mulberry) is one of the most important medicine and food crops in China and other developing countries in Asia due to its large biomass (twigs) and useful by-products. Additionally, its twigs are an excellent green resource for bioactive compounds that are resistant to ultraviolet (UV) radiation and that can whiten skin and scavenge free radicals, and are of potential interest to the cosmetics and health food industries. In this study, we evaluated the ability of Morus alba L. twig hydroethanol extract and isolated compounds to protect DNA against UV damage. Agarose gel electrophoresis showed that a hydroethanol extract and six different polyphenol monomers (oxyresveratrol, resveratrol, mulberroside A, rutin, quercetin, and morin) present in the extract protected pUC19 plasmid DNA from UV damage. The optimal concentrations of polyphenols and Morus alba L. twig extract for DNA protection were 0.500 mg/mL and 1.00 mg/mL, respectively. These compounds may have potential as skin-care products to prevent UV-induced damage to the skin. Additionally, activity contribution degree assessment revealed other Morus alba L. substances possessing significant DNA protection activity that are worthy of further investigation. ARTICLE HISTORY

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“…The health‐promoting properties of these bioactive compounds contribute to the nutritional value of crops such as tomato and represent a parameter of preference in consumer choice of food products. Indeed, some of these bioactive compounds, such as AsA and carotenoids, cannot be synthesized by animals and thus have to be introduced with the diet . For these reasons, the daily consumption of plant‐derived food enriched in these compounds has been highly promoted in the last decade.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, some of these bioactive compounds, such as AsA and carotenoids, cannot be synthesized by animals and thus have to be introduced with the diet. 18 For these reasons, the daily consumption of plant-derived food enriched in these compounds has been highly promoted in the last decade.…”

Section: Introductionmentioning

confidence: 99%

Selection of tomato landraces with high fruit yield and nutritional quality under elevated temperatures

et al. 2020

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BACKGROUND Global warming and extreme or adverse events induced by climatic fluctuations are an important threat for plants growth and agricultural production. Adaptability to environmental changes prevalently derives from a large set of genetic traits affecting physiological and agronomic parameters. Therefore, the identification of genotypes that are good yield performer at high temperatures is becoming increasingly necessary for future breeding programs. Here, we analyzed the performances of different tomato landraces grown under elevated temperatures in terms of yield and nutritional quality of the fruit. Finally, we evaluated the antioxidant and anti‐inflammatory activities of fruit extracts from the tomato landraces selected. RESULTS The tomato landraces analyzed here in a hot climate differed in terms of yield performance, physicochemical parameters of fruit (pH, titratable acidity, degrees Brix, firmness), bioactive compounds (ascorbic acid, carotenoids, and polyphenols), and anti‐inflammatory potential. Three of these landraces (named E30, E94, and PDVIT) showed higher fruit quality and nutritional value. An estimated evaluation index allowed identification of PDVIT as the best performer in terms of yield and fruit quality under high temperatures. CONCLUSION The analyses performed here highlight the possibility to identify new landraces that can combine good yield performances and fruit nutritional quality at high temperatures, information that is useful for future breeding programs. © 2020 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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An ascorbic acid-enriched tomato genotype to fight UVA-induced oxidative stress in normal human keratinocytes

Cited by 49 publications

References 31 publications

Protective effects of novel derivatives of vitamin D3 and lumisterol against UVB-induced damage in human keratinocytes involve activation of Nrf2 and P53 defense mechanisms

Protective effects of novel derivatives of vitamin D3 and lumisterol against UVB-induced damage in human keratinocytes involve activation of Nrf2 and P53 defense mechanisms

DNA protection activity of a hydroethanol extract and six polyphenol monomers fromMorus alba L. (mulberry) twig

Selection of tomato landraces with high fruit yield and nutritional quality under elevated temperatures

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