An Aspergillus nidulans endo-β-1,3-glucanase exhibited specific catalytic features and was used to prepare 3-O-β-cellobiosyl-d-glucose and 3-O-β-gentiobiosyl-d-glucose with high antioxidant activity from barley β-glucan and laminarin, respectively

Liu, Cuicui; Yan, Songling; Zhao, Jing; Lin, Miao; Duan, Baiyun; Zhang, Zhenqing; Yang, Yao; Z, Liu; Yuan, Sheng

doi:10.1016/j.ijbiomac.2021.07.053

Cited by 6 publications

(3 citation statements)

References 47 publications

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“…Due to its stable, straightforward, quick, sensitive, and repeatable process, the DPPH technique is frequently used to assess the antioxidant activity of substances. AnENG16A‐hydrolysate of barley β‐glucan had a 7.39‐fold higher DPPH activity than undigested barley β‐glucan, according to Liu et al ., however, the duration of the hydrolysis process was not mentioned in the article (Liu et al ., 2021). Antioxidant activity is found in Porphyra yezoensis , and polysaccharides, polyphenols, proteins and porphyrins are found to be active compounds.…”

Section: Resultsmentioning

confidence: 99%

“…The β‐glucan properties in cereals are modulated by several hydrolytic enzymes, with β‐glucanase (also called endo‐b‐1,3; 1,4‐ glucanase, 1,3‐1,4‐b‐D‐glucan 4‐glucanohydrolase; GH family 17; EC 3.2.1.73) as the most important among them (Ribeiro et al ., 2011). Enzymatic modification, such as β‐glucans depolymerisation, is an important approach for the improvement of the bioactive properties of these polysaccharides (Liu et al ., 2021). Microbial β‐glucanases, called endo‐β‐1,3‐1,4‐glucanase or lichenase (GH family 16; EC 3.2.1.73), are produced by various microorganisms, including bacteria and fungi (Niu et al ., 2016; Zhang et al ., 2017; Yan et al ., 2018; Liu et al ., 2020).…”

Section: Introductionmentioning

confidence: 99%

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Improvement of nutritional and bioactive properties of barley β‐glucan‐based food products using Bacillus subtilis 168 endo‐β‐1,3‐1,4‐glucanase

Kojić

Margetić

Ristović

et al. 2023

Int J of Food Sci Tech

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SummaryThe combination of β‐oligosaccharides from enzymatically‐hydrolyzed barley β‐glucan has attracted interest recently due to its positive effects on human health. This study aimed to assess the impact of the endo‐β‐1,3‐1,4‐glucanase enzyme from Bacillus subtilis 168 on improving the nutritional and bioactive properties of barley β‐glucan. A new procedure for the isolation of β‐glucan was developed, at a lower temperature (45°C), enabling purity from starch contamination, without affecting the yield (6 g β‐glucan from 100 g of barley flour). The endo‐β‐1,3‐1,4‐glucanase is cloned into E. coli pQE_Ek enables the high production and purification (82% yield, 1.8 mg mL‐1 and 440 U mg‐1) of an enzyme identical to the natural one (25.5 kDa). The enzymatic reaction showed high efficiency of β‐glucan degradation by recombinant enzyme, giving a mixture of products (of which 3‐O‐β‐cellobiosyl‐D‐glucose and 3‐O‐β‐cellotriosyl‐D‐glucose are the most abundant), the reduction of viscosity (17%) and increase in antioxidant capacities by 15.2%, 30.9% and 44.0% assessed by ABTS, DPPH and ORAC, respectively. These results indicate the possible application of endo‐β‐1,3‐1,4‐glucanase enzyme in improving the properties of barley β‐glucan used as functional foods.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improvement of nutritional and bioactive properties of barley β‐glucan‐based food products using Bacillus subtilis 168 endo‐β‐1,3‐1,4‐glucanase

Kojić

Margetić

Ristović

et al. 2023

Int J of Food Sci Tech

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“…The kinetic parameters of CcMan5C were determined using 0.25 mg.mL −1 –5 mg.mL −1 LBG instead of 2.5 mg.mL −1 LBG. The K m and V max were determined by plotting the hydrolytic rate against the LBG concentration using OriginPro 8 SR0 (OriginLab Corporation, Northampton, MA, USA) [ 42 ]. For analysis of the effect of organic solvents or detergents on the hydrolytic activity of CcMan5C, 10% or 20% indicated organic solvents or 1% or 10% indicated detergents were added to the above reaction mixture.…”

Section: Methodsmentioning

confidence: 99%

Heterologous Expression, Purification and Characterization of an Alkalic Thermophilic β-Mannanase CcMan5C from Coprinopsis cinerea

Yan

Duan

Liu

et al. 2023

JoF

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A endo-1,4-β-mannanase (CcMan5C) gene was cloned from Coprinopsis cinerea and heterologously expressed in Pichia pastoris, and the recombinant enzyme was purified by Ni-affinity chromatography and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF-MS). CcMan5C hydrolyzed only locust bean gum galactomannan (LBG) but not α-mannan from S. cerevisiae or Avicel cellulose, oat spelt xylan, or laminarin from Laminaria digitata. CcMan5C exhibited distinctive catalytic features that were different from previously reported β-mannanases. (1) CcMan5C is the first reported fungal β-mannase with an optimal alkalic pH of 8.0–9.0 for hydrolytic activity under assay conditions. (2) CcMan5C is the first reported alkalic fungal β-mannase with an optimal temperature of 70 °C for hydrolytic activity under assay conditions. (3) The organic solvents methanol, ethanol, isopropanol, and acetone at concentrations of 10% or 20% did not inhibit CcMan5C activity, while 10% or 20% isopropanol and acetone even enhanced CcMan5C activity by 9.20–34.98%. Furthermore, CcMan5C tolerated detergents such as Tween 20 and Triton X-100, and its activity was even enhanced to 26.2–45.6% by 1% or 10% Tween 20 and Triton X-100. (4) CcMan5C solution or lyophilized CcMan5C exhibited unchanged activity and even increasing activity after being stored at −20 °C or −80 °C for 12 months and retained above 50% activity after being stored at 4 °C for 12 months. These features make CcMan5C a suitable candidate for the detergent industry and paper and pulp industry.

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Microbial β-glucanases: production, properties, and engineering

Wang

2023

World J Microbiol Biotechnol

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An Aspergillus nidulans endo-β-1,3-glucanase exhibited specific catalytic features and was used to prepare 3-O-β-cellobiosyl-d-glucose and 3-O-β-gentiobiosyl-d-glucose with high antioxidant activity from barley β-glucan and laminarin, respectively

Cited by 6 publications

References 47 publications

Improvement of nutritional and bioactive properties of barley β‐glucan‐based food products using Bacillus subtilis 168 endo‐β‐1,3‐1,4‐glucanase

Improvement of nutritional and bioactive properties of barley β‐glucan‐based food products using Bacillus subtilis 168 endo‐β‐1,3‐1,4‐glucanase

Heterologous Expression, Purification and Characterization of an Alkalic Thermophilic β-Mannanase CcMan5C from Coprinopsis cinerea

Microbial β-glucanases: production, properties, and engineering

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