An Assay Method for Nitric Oxide-Related Compounds in Whole Blood

Sonoda, Masaru; Kobayashi, Jun; Takezawa, Masue; Miyazaki, Takashi; Nakajima, Takanori; Shimomura, Hiroji; Koike, Kazuyuki; Satomi, Akira; Ogino, Hiroshi; Omoto, Ryozo; Komoda, Tsugikazu

doi:10.1006/abio.1997.2056

Cited by 41 publications

(28 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several laboratories have reported the use of the filters mentioned in this work as a sample preparation procedure before analyzing urine and to remove large molecular weight macromolecules, such as hemoglobin, from serum samples (1,2). In this report, we expanded those observations to include normal human serum and FBS-supplemented tissue culture medium.…”

Section: Resultsmentioning

confidence: 73%

“…Fax: (210) 916-1460. 2 To whom correspondence should be addressed. the measurement of nitrite/nitrate levels in body fluids and biological and cellular extracts, from both human and animal subjects.…”

mentioning

confidence: 98%

“…The importance of nitric oxide (NO) 3 as a mediator in many molecular events is underscored by its role in physiological processes such as vasodilation, sepsis, thrombosis, immune function, and neurotransmission (1,2). NO is biosynthesized from the amino acid Larginine by the action of nitric oxide synthase (NOS).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Elimination of Matrix-Based Interferences to a Fluorescent Nitrite/Nitrate Assay by a Simple Filtration Procedure

Bedwell

Rivera

Merrill

2000

Analytical Biochemistry

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 73%

mentioning

confidence: 98%

See 1 more Smart Citation

Elimination of Matrix-Based Interferences to a Fluorescent Nitrite/Nitrate Assay by a Simple Filtration Procedure

Bedwell

Rivera

Merrill

2000

Analytical Biochemistry

View full text Add to dashboard Cite

“…This approach relies on the extraction of NO from selective species, either chemically or optically, and their subsequent detection with an NO chemiluminescence detector where trace levels of NO are detected in the gas phase through its chemiluminescent reaction with ozone (Fontijn, 1970). When coupled to the gas efflux from purge vessels containing reducing/oxidizing solutions or from UV irradiated capillaries, this detector can quantify NO products, including nitrite and nitrate (Walters et al, 1978;Cox and Frank, 1982;Dunham et al, 1995;Yang et al, 1997;Trushina et al, 1997), nitroso products (Massey et al, 1984;Stamler et al, 1992;Samouilov and Zweier, 1998;Ewing and Janero, 1998;Gladwin et al, 2000;Rassaf et al, 2002;Jourd'heuil et al, 2005;Wang et al, 2005;Zhang et al, 2005), nitrosyl-hemes (Gladwin et al, 2000;Feelisch et al, 2002) and total NO (Sonoda et al, 1997). The wide use of NO chemiluminescence detectors in life science laboratories can be attributed to several factors, including sensitivity, selectivity, quick response, ease of use, and the relatively moderate pricing and space requirements that are comparable to other commonly used laboratory instruments.…”

Section: Introductionmentioning

confidence: 99%

Isotope tracing enhancement of chemiluminescence assays for nitric oxide research

Cornelius

Tran

Turner

et al. 2008

Biological Chemistry

View full text Add to dashboard Cite

Chemiluminescence assays are used widely for the detection of nitric oxide (NO)-derived species in biological fluids and tissues. Here, we demonstrate that these assays can be interfaced with mass-sensitive detectors for parallel determination of isotopic abundance. Results obtained with tri-iodide and ascorbic acid-based reductive assays indicate that mass spectrometric detection enables NO isotope-tracing experiments to be carried out to a limit of detectability of a few picomoles, a sensitivity similar to that of standard gas phase chemiluminescence methods. The advantage afforded by mass spectrometric detection is demonstrated using the murine macrophage cell line J774, which is shown here to reduce15NO3-to15NO2-under anoxic conditions. The particular combination of an analytical and cellular system described here may hold promise for future characterization of the enzymatic pathways contributing to mammalian nitrate reductase activity, without background interference from14NO2-derived from other sources.

show abstract

“…Of these, two are constitutive isoforms; the third, inducible and Ca 2ϩ -independent NO synthase (iNOS), is expressed only following transcriptional activation of its gene (16,41), as occurs in acute and chronic inflammation (10). Biosynthesis of NO has been increasingly recognized as an important intra-and intercellular messenger molecule in vascular relaxation, platelet activation, and immune responses (27) in human mononuclear cells. It plays important roles in the pathogenesis of septic shock caused by gram-negative bacteria and of other infectious disease sequelae (37).…”

mentioning

confidence: 99%

Activation of Nuclear Factor κB and Induction of Inducible Nitric Oxide Synthase byUreaplasma urealyticumin Macrophages

Yan

Jensen

et al. 2000

Infect Immun

View full text Add to dashboard Cite

Chronic lung disease (CLD) of prematurity is an inflammatory disease with a multifactorial etiology. The importance of Ureaplasma urealyticum in the development of CLD is debated, and steroids produce some improvement in neonates with this disease. In the present study, the capability of U. urealyticum to stimulate rat alveolar macrophages to produce nitric oxide (NO), express inducible nitric oxide synthase (iNOS), and activate nuclear factor B (NF-B) in vitro was characterized. The effect of NO on the growth of U. urealyticum was also investigated. In addition, the impact of dexamethasone and budesonide on these processes was examined. We found that U. urealyticum antigen (>4 ؋ 10 7 color-changing units/ml) stimulated alveolar macrophages to produce NO in a dose-and time-dependent manner (P < 0.05). This effect was further enhanced by gamma interferon (100 IU/ml; P < 0.05) but was attenuated by budesonide and dexamethasone (10 ؊4 to 10 ؊6 M) (P < 0.05). The mRNA and protein levels of iNOS were also induced in response to U. urealyticum and inhibited by steroids. U. urealyticum antigen triggered NF-B activation, a possible mechanism for the induced iNOS expression, which also was inhibited by steroids. NO induced by U. urealyticum caused a sixfold reduction of its own growth after infection for 10 h. Our findings imply that U. urealyticum may be an important factor in the development of CLD. The host defense response against U. urealyticum infection may also be influenced by NO. The down-regulatory effect of steroids on NF-B activation, iNOS expression, and NO production might partly explain the beneficial effect of steroids in neonates with CLD.

show abstract

An Assay Method for Nitric Oxide-Related Compounds in Whole Blood

Cited by 41 publications

References 49 publications

Elimination of Matrix-Based Interferences to a Fluorescent Nitrite/Nitrate Assay by a Simple Filtration Procedure

Elimination of Matrix-Based Interferences to a Fluorescent Nitrite/Nitrate Assay by a Simple Filtration Procedure

Isotope tracing enhancement of chemiluminescence assays for nitric oxide research

Activation of Nuclear Factor κB and Induction of Inducible Nitric Oxide Synthase byUreaplasma urealyticumin Macrophages

Contact Info

Product

Resources

About