An assessment of genetic relationships within the genus Digitalis based on PCR-generated RAPD markers

Nebauer, Sergio G.; Castillo-Agudo, Lucas del; Segura, Juan

doi:10.1007/s001220051426

Cited by 43 publications

(40 citation statements)

References 30 publications

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“…Except at some point there was significant associations between the dendrogram obtained by RAPD markers and morphological characteristics. This was in accordance with the results reported by Nebauer et al (2000) on the genus Digitalis and Thakur et al (2008) on maize. The notion is that the distribution of genetic variation using RAPD analysis will help in identification of superior genotype for cultivar upgrading as well as to evolve breeding strategies for genetic improvement in Amaranthus spp.…”

Section: Assessment Of Relationship Between Morphological and Moleculsupporting

confidence: 94%

Assessment of the morphological and molecular diversity in Amaranthus spp.

Anjali¹,

Joshi²,

Maloo³

et al. 2013

Afr. J. Agric. Res.

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Section: Assessment Of Relationship Between Morphological and Moleculsupporting

confidence: 94%

Assessment of the morphological and molecular diversity in Amaranthus spp.

Anjali¹,

Joshi²,

Maloo³

et al. 2013

Afr. J. Agric. Res.

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“…Random amplified polymorphic DNA (RAPD) technology (Williams et al, 1990) is a means of investigating genetic diversity within and between populations and has been applied to many plants (Nebauer et al, 1999(Nebauer et al, , 2000Ester et al, 2001;Jover et al, 2003). Once established, RAPD-PCR (polymerase chain reaction) has the advantage of being quick and easy, requiring little plant material, and having a high resolution (Gugerli et al, 1999;Bronzini et al, 2002).…”

Section: Introductionmentioning

confidence: 97%

RAPD Profiling in Detecting Genetic Variation in Endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China

et al. 2005

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Random amplified polymorphic DNA (RAPD) markers were used to measure genetic diversity of Coelonema draboides (Brassicaceae), a genus endemic to the Qilian Mountains of the Qinghai-Tibet Plateau. We sampled 90 individuals in 30 populations of Coelonema draboides from Datong and Huzhu counties of Qinghai Province in P.R. China. A total of 186 amplified bands were scored from the 14 RAPD primers, with a mean of 13.3 amplified bands per primer, and 87% (161 bands) polymorphic bands (PPB) was found. Analysis of molecular variance (AMOVA) shows that a large proportion of genetic variation (84.2%) resides among individuals within populations, while only 15.8% resides among populations. The species shows higher genetic diversity between individuals than other endemic and endangered plants. The RAPDs provide a useful tool for assessing genetic diversity of rare, endemic species and for resolving relationships among populations. The results show that the genetic diversity of this species is high, possibly allowing it to adapt more easily to environmental variations. The main factor responsible for the high level of differentiation within populations and the low level of diversity among populations is probably the outcrossing and long-lived nature of this species. Some long-distance dispersal, even among far separated populations, is also a crucial determinant for the pattern of genetic variation in the species. This distributive pattern of genetic variation of C. draboides populations provides important baseline data for conservation and collection strategies for the species. It is suggested that only populations in different habitats should be studied and protected, not all populations, so as to retain as much genetic diversity as possible.

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“…In order to investigate the genetic diversity among closely related species, among subspecies or among populations, random amplified polymorphic DNA (RAPD) analysis was successfully applied in several species (Khandka et al 1997;Mengoni et al 2000;Mneney et al 2001;Neubauer et al 2000;Qian et al 2000;Baumbach and Hellwig 2003). The genetic variability of A. tripolium growing at inland habitats in Thuringia and Saxony-Anhalt has already been successfully analyzed by RAPDs (Krü ger et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Genetic variation among different populations of Aster tripolium grown on naturally and anthropogenic salt-contaminated habitats: implications for conservation strategies

et al. 2006

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The sea aster, Aster tripolium L., grows naturally in temperate regions, mainly in the salt meadows close to the coast. The species is also found in naturally and anthropogenically salt-contaminated inland habitats, such as potash mine dumps. The genetic relationships among populations from different habitats and correlations of the genotype with physiological and vegetational parameters were investigated. A. tripolium plants from five different sites close to the seashore on the North Sea island Baltrum, from five different potash mine dumps and, as an outgroup, from the seashore in Japan were probed. DNA was extracted from five plants from each of the 11 A. tripolium populations and analyzed for random amplified polymorphic DNA (RAPD). Altogether 35 polymorphic bands in 51 individuals and 45 different detectable genotypes could be identified. For evaluation of the genetic variation using RAPD bands, the neighbor-joining method, the principal coordinate analysis, and the analysis of molecular variance were applied, resulting in the classification into three genetic groups. A. tripolium plants from different ecological habitats on Baltrum were closely related while the plants growing at the deposit dumps showed a higher genetic diversity. The Japanese population was genetically very different from the German populations. Correlations between phytosociological and soil parameters and the respective genotype were not significant. The results argue for a conservation of anthropogenically salt-contaminated habitats to maintain genetic variability not only on the species level, but also within a species.

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An assessment of genetic relationships within the genus Digitalis based on PCR-generated RAPD markers

Cited by 43 publications

References 30 publications

Assessment of the morphological and molecular diversity in Amaranthus spp.

Assessment of the morphological and molecular diversity in Amaranthus spp.

RAPD Profiling in Detecting Genetic Variation in Endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China

Genetic variation among different populations of Aster tripolium grown on naturally and anthropogenic salt-contaminated habitats: implications for conservation strategies

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