An assessment of somaclonal variation in micropropagated plants of sugarcane by RAPD markers

Abstract

Sugarcane variety Co 94012 is a new variety released in India through the use of somaclonal variation. Significant differences were found in quantitative and qualitative agronomic characters. Another somaclone VSI 434 is an extra early maturing variety with significant difference in morphological and qualitative characters over the parent CoC 671. The genetic variation of micropropagated plantlets was tested by RAPD analysis. The banding pattern of PCR amplified products from micropropagated plantlets showed that most of them were monomorphic in both the varieties. The amplicon pattern of VSI 434 and Co 94012 with primers OPA 17 and OPA 19 differed from parent CoC 671, respectively. But with other primers they were more or less monomorphic indicating that the amplicon from these two primers can be used as potential fingerprint for Co VSI 434 and Co 94012.

Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers

“…The present data are similar to reports of others. There are a number of findings in the literature which report similar results for detection of genetic fidelity using molecular markers in different crops such as Solanum aculeatissimum (Ghimire et al 2012), Olea europaea (Leva and Petruccelli 2012), Saccharum officinarum (Tawar et al 2008), Dendrocalamus hamiltonii (Agnihotri et al 2009), Capparis deciduas (Tyagi et al 2010), Vanilla spp. (Sreedhar et al 2007), Dioscorea bulbifera (Narula et al 2007) and Musa spp.…”

EfficientIn VitroRegeneration of Sugarcane (Saccharum OfficinarumL.) from Bud Explants

“…Despite the interest in S. officinarum, there are only a few earlier reports on its regeneration through shoot tip cultures (11,17,18,34), apical meristem (10,33), leaf sheath (14), shoot tops (35), young leaf segments (19), nodal buds (8), young meristematic leaf sheath (31), immature leaf explants (39), leaf spindle explants, germinated seeds (9), shoot apex (22), tops with growing apices (30) The aim of the present study was to develop an efficient, reliable and promising protocol for regeneration of S. officinarum from bud explants.…”

Applications of in vitro culture systems for commercial sugarcane production and improvement

“…Such variation may be attributed to existing DNA polymorphism (Tawar et al 2008), but, because most analyses have low coverage of the sugarcane genome (Taylor et al 1995), these differences may not result in any phenotypic manifestation. In our laboratories, although plants produced through indirect somatic embryogenesis showed more polymorphic bands (0.5-0.7%) than conventionally propagated (setts) controls, they were phenotypically similar regarding stalk mass, diameter, height, population density and sucrose yield after 9 mo in the field (Watt et al 2009).…”