An assessment of the overexpression of BMP‐2 in transfected human osteoblast cells stimulated by mineral trioxide aggregate and Biodentine

Abstract

Aim To evaluate the effect of MTA and Biodentine on viability, osteogenic differentiation and BMP-2 expression in osteogenic cells. Methodology Saos-2 cells were used as a model of osteoblastic cells. Overexpression of BMP-2 was induced by transfection of a CMV-driven plasmid construct including the human BMP-2 coding sequence, and stably transfected cells were selected. Cell viability was assessed by the mitochondrial dehydrogenase enzymatic (MTT) assay. The bioactivity of the materials was evaluated by the alkaline phosphatase (ALP) assay and detection of calcium deposits with alizarin red staining (ARS). The gene expression of BMP-2 and ALP was quantified with real-time PCR. Statistical analysis was performed with analysis of variance and Bonferroni or Tukey post-test (a = 0.05). Results Viability tests revealed that MTA and Biodentine were not cytotoxic at the higher dilution (1 : 8) to BMP-2-transfected cells. MTA and Biodentine exhibited the highest ALP activity when compared to the Saos-BMP-2-unexposed control group (P < 0.05). Cell exposure to Biodentine and MTA had a significant stimulatory effect on the formation of mineralized nodules (P < 0.05). The highest increase in BMP-2 gene expression was observed after 3 days of BMP-2-transfected cells exposure to MTA and Biodentine in non-osteogenic medium in relation to Saos-BMP-2-unexposed control cells (P < 0.05). Exposure of cells to MTA in osteogenic medium for 1 day increased ALP gene expression by approximately 1.3-fold in relation to Saos-BMP-2-unexposed control cells (P < 0.05). Conclusions Both MTA and Biodentine showed biocompatibility and bioactivity in Saos-BMP-2 overexpressing cells. Biodentine had a significantly greater effect on mineralization than MTA. Both MTA and Biodentine enhanced BMP-2 mRNA expression in the transfected system. Both MTA and Biodentine are suitable materials to improve osteoblastic cell mineralization.

Differential Gene Expression Changes in Human Primary Dental Pulp Cells Treated with Biodentine and TheraCal LC Compared to MTA

“…Thus, it appears that both resin monomers and light-curing could be responsible for the different biological effects of TC against those of MTA and BD. BMP2 expression was also found to be highly up-regulated in BD-treated cells at 72 h, consistent with a previous study using human osteoblast cells [55].…”

Cytotoxicity of Different Concentrations of Three Root Canal Sealers on Human Mesenchymal Stem Cells

“…In addition, the composition of BR resembles that of Biodentine (Septodont, Saint Maur Des Fosses, France), a tricalcium silicate-based cement, which can be used as a dentine replacement material in the crown and in the root. A drop in cell viability after exposure to high concentrations of Biodentine extracts was previously reported [ 24 , 25 ]. It has been stated that this could be caused by the release of calcium ions and the increase in pH [ 26 ].…”

Histopathological, histoenzymological, immunohistochemical and immunofluorescence analysis of tissue response to sealing materials after furcation perforation

“…Production of OCN by osteoblastic cells in contact with MTA has been demonstrated in vitro (Tani‐Ishii et al ). In addition, MTA has been shown to induce the expression of the BMP‐2 and OCN osteogenic markers in dental pulp cells (Rodrigues et al ) and both MTA and Biodentine™ induced BMP‐2 expression in osteogenic cells and were considered adequate materials to stimulate the mineralization by osteoblastic cells (Rodrigues et al ). BSP is also involved in the formation of the mineralized matrix, and the expression of this protein is highly specific for mineralized tissues including bone, cartilage, dentine and cementum (Tani‐Ishii et al ).…”