An association study of thrombospondin 1 and 2 SNPs with coronary artery disease and myocardial infarction among South Indians

Manickaraj, Ashok Kumar; Chakrapani, Anbarasan; SaiBabu, Ramineni; Kuram, Sriram; Raman, Suresh C; Cherian, Koothurathu Mammen

doi:10.1016/j.thromres.2011.05.026

Cited by 13 publications

(17 citation statements)

References 30 publications

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“…28 TSP-1 has further been detected in atherosclerotic specimens, and the genetic variants of TSP-1 have been reported to correlate with coronary artery disease and myocardial infarction. [29][30][31] In Apoe -/-mice, TSP-1 deficiency has also been shown to accelerate atherosclerotic plaque maturation without affecting plaque formation. 32 In addition to the inhibition of endothelial recovery, we further ask whether TSP-1 mediates ADAMTS-7 promotion of VSMCs migration.…”

Section: Discussionmentioning

confidence: 99%

ADAMTS-7 Inhibits Re-endothelialization of Injured Arteries and Promotes Vascular Remodeling Through Cleavage of Thrombospondin-1

et al. 2015

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E ndothelial cells (ECs) play an essential role in the modulation of vascular homeostasis. During aging and specifically during the development of atherosclerosis, ECs are exposed to various damaging stimuli and are thereby prone to injury.1 Rapid endothelial recovery, or re-endothelialization, correlates with diminished plaque formation. 2 Likewise, coronary intervention-induced vascular injury requires an Background-ADAMTS-7, a member of the disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family, was recently identified to be significantly associated genomewide with coronary artery disease. However, the mechanisms that link ADAMTS-7 and coronary artery disease risk remain elusive. We have previously demonstrated that ADAMTS-7 promotes vascular smooth muscle cell migration and postinjury neointima formation via degradation of a matrix protein cartilage oligomeric matrix protein. Because delayed endothelium repair renders neointima and atherosclerosis plaque formation after vessel injury, we examined whether ADAMTS-7 also inhibits re-endothelialization. Methods and Results-Wire injury of the carotid artery and Evans blue staining were performed in Adamts7-/-and wild-type mice. Adamts-7 deficiency greatly promoted re-endothelialization at 3, 5, and 7 days after injury. Consequently, Adamts-7 deficiency substantially ameliorated neointima formation in mice at days 14 and 28 after injury in comparison with the wild type. In vitro studies further indicated that ADAMTS-7 inhibited both endothelial cell proliferation and migration. Surprisingly, cartilage oligomeric matrix protein deficiency did not affect endothelial cell proliferation/migration and re-endothelialization in mice. In a further examination of other potential vascular substrates of ADAMTS-7, a labelfree liquid chromatography-tandem mass spectrometry secretome analysis revealed thrombospondin-1 as a potential ADAMTS-7 target. The subsequent studies showed that ADAMTS-7 was directly associated with thrombospondin-1 by its C terminus and degraded thrombospondin-1 in vivo and in vitro. The inhibitory effect of ADAMTS-7 on postinjury endothelium recovery was circumvented in Tsp1 -/-mice. Conclusions-Our study revealed a novel mechanism by which ADAMTS-7 affects neointima formation. Thus, ADAMTS-7 is a promising treatment target for postinjury vascular intima hyperplasia. potentially because they inhibit not only vascular smooth muscle cell (VSMC) proliferation/migration, but also reendothelialization. 6,7 Therefore, new strategies that aim to promote endothelial recovery, and simultaneously inhibit VSMC activation, as well, are needed for the effective prevention and treatment of atherosclerosis and postinjury restenosis.Metalloproteinases are critical in vascular wall remodeling through matrix or nonmatrix degradation. 8 Recently, we described ADAMTS-7, a member of a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family, in the mediation of VSMC migration and the promotion of neointima formation following ar...

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Section: Discussionmentioning

confidence: 99%

ADAMTS-7 Inhibits Re-endothelialization of Injured Arteries and Promotes Vascular Remodeling Through Cleavage of Thrombospondin-1

et al. 2015

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“…Although the correlations of the THBS1 N700S or THBS4 A387P coding polymorphisms with premature myocardial infarction have not been replicated in populations from other regions (e.g. Koch et al, 2008;Ashokkumar et al, 2011), the risk associated with the THBS1 N700S polymorphism has been replicated in an Italian population (Zwicker et al, 2006).…”

Section: The Thrombospondin (Tsp) Family: Domains Structures and Funmentioning

confidence: 94%

Adhesion-modulating/matricellular ECM protein families: A structural, functional and evolutionary appraisal

Mosher

Adams

2012

Matrix Biology

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“…In total, 13 studies [8][9][10][11][12][13][23][24][25][26][27][28][29] were included in the current metaanalysis. Among the 13 studies, eight investigated the THBS1 N700S polymorphism [8,9,12,[23][24][25]28,29]; five investigated the polymorphism located in THBS2 3'UTR [8,23,24,28,29] and nine investigated the THBS4 A387P polymorphism [8][9][10][11]23,24,[26][27][28].…”

Section: Characteristics Of Eligible Studiesmentioning

confidence: 99%

“…Among the 13 studies, eight investigated the THBS1 N700S polymorphism [8,9,12,[23][24][25]28,29]; five investigated the polymorphism located in THBS2 3'UTR [8,23,24,28,29] and nine investigated the THBS4 A387P polymorphism [8][9][10][11]23,24,[26][27][28]. Six were performed in an American population [8,10,13,23,26,27].…”

Section: Characteristics Of Eligible Studiesmentioning

confidence: 99%

“…Six were performed in an American population [8,10,13,23,26,27]. Three were performed in a European population [9,24,28] and four were performed in an Asian population [11,12,25,29]. Repetition of partial/total tested samples with a different method 2 Repetition of partial/total tested samples with the same method 1 Not described 0 5.…”

Section: Characteristics Of Eligible Studiesmentioning

confidence: 99%

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Association between single nucleotide polymorphisms in thrombospondins genes and coronary artery disease: A meta-analysis

Zhang¹,

Wei²,

Li³

et al. 2015

Thrombosis Research

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An association study of thrombospondin 1 and 2 SNPs with coronary artery disease and myocardial infarction among South Indians

Cited by 13 publications

References 30 publications

ADAMTS-7 Inhibits Re-endothelialization of Injured Arteries and Promotes Vascular Remodeling Through Cleavage of Thrombospondin-1

ADAMTS-7 Inhibits Re-endothelialization of Injured Arteries and Promotes Vascular Remodeling Through Cleavage of Thrombospondin-1

Adhesion-modulating/matricellular ECM protein families: A structural, functional and evolutionary appraisal

Association between single nucleotide polymorphisms in thrombospondins genes and coronary artery disease: A meta-analysis

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