An Atlas of Transcription Factors Expressed in Male Pupal Terminalia of<i>Drosophila melanogaster</i>

Vincent, Ben J.; Rice, Gavin; Wong, Gabriella M.; Glassford, William J.; Downs, Kayla I.; Shastay, Jessica L.; K, Charles-Obi; Natarajan, Malini; Gogol, Madelaine; Zeitlinger, Julia; Rebeiz, Mark

doi:10.1534/g3.119.400788

Cited by 21 publications

(27 citation statements)

References 100 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with a previous study (Vincent et al, 2019), we found that h is ubiquitously expressed throughout the male genitalia of D. melanogaster, including the clasper primordia ( Fig. 3B).…”

Section: Interactions Between Genes Underlying Clasper Divergencesupporting

confidence: 92%

“…However, h is not differentially expressed between Dsim w 501 and Dmau D1 and appears to be ubiquitously expressed in the developing genitalia of D. melanogaster (Fig. 3B) (Vincent et al, 2019).…”

Section: Functional Analysis Of Expressed Positional Candidates On Chmentioning

confidence: 92%

“…were differentially expressed ( Supplementary File 6). This includes 61 out of the 802 TF encoding genes, five of which have been shown to be expressed in both the claspers and the posterior lobes of D. melanogaster (hinge3, Myb oncogene-like, single stranded-binding protein c31A, Sox21b and Enhancer of split m3, helix-loop-helix) (Vincent et al, 2019). This suggests that the regulatory landscape of developing genitalia is generally conserved between D.…”

Section: Genome-wide Gene Expression During Genital Development In Dmentioning

confidence: 99%

See 2 more Smart Citations

Unravelling the genetic basis for the rapid diversification of male genitalia betweenDrosophilaspecies

Hagen

Mendes

Booth

et al. 2020

Preprint

View full text Add to dashboard Cite

Corresponding authors: msantos-nunes@brookes.ac.uk (MDSN) and amcgregor@brookes.ac.uk (APM). AbstractIn the last 240,000 years, males of the Drosophila simulans species clade have evolved striking differences in the morphology of their epandrial posterior lobes and claspers (surstyli). These changes have most likely been driven by sexual selection and mapping studies indicate a highly polygenic and generally additive genetic basis. However, we have limited understanding of the gene regulatory networks that control the development of genital structures and how they evolved to result in this rapid phenotypic diversification. Here, we used new D. simulans / D. mauritiana introgression lines on chromosome 3L to generate higher resolution maps of posterior lobe and clasper differences between these species. We then carried out RNA-seq on the developing genitalia of both species to identify the genes expressed during this process and those that are differentially expressed between the two species. This allowed us to test the function of expressed positional candidates during genital development in D. melanogaster. We identified several new genes involved in the development and possibly the evolution of these genital structures, including the transcription factors Hairy and Grunge. Furthermore, we discovered that during clasper development Hairy negatively regulates tartan, a gene known to contribute to divergence in clasper morphology. Taken together our results provide new insights into the regulation of genital development and how this evolves between species.

show abstract

Section: Interactions Between Genes Underlying Clasper Divergencesupporting

confidence: 92%

Section: Functional Analysis Of Expressed Positional Candidates On Chmentioning

confidence: 92%

Section: Genome-wide Gene Expression During Genital Development In Dmentioning

confidence: 99%

See 1 more Smart Citation

Unravelling the genetic basis for the rapid diversification of male genitalia betweenDrosophilaspecies

Hagen

Mendes

Booth

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Finally, in situ hybridization was carried out as previously described (Vincent et al. 2019). Briefly, we used an InsituPro VSi robot to rehydrate in PBT, fix in PBT with 4% PFA, and prehybridize in hybridization buffer for 1 h at 65°C.…”

Section: Methodsmentioning

confidence: 99%

Co‐evolving wing spots and mating displays are genetically separable traits in Drosophila

et al. 2020

Self Cite

View full text Add to dashboard Cite

The evolution of sexual traits often involves correlated changes in morphology and behavior. For example, in Drosophila, divergent mating displays are often accompanied by divergent pigment patterns. To better understand how such traits co‐evolve, we investigated the genetic basis of correlated divergence in wing pigmentation and mating display between the sibling species Drosophila elegans and Drosophila gunungcola. Drosophila elegans males have an area of black pigment on their wings known as a wing spot and appear to display this spot to females by extending their wings laterally during courtship. By contrast, D. gunungcola lost both of these traits. Using Multiplexed Shotgun Genotyping (MSG), we identified a ∼440 kb region on the X chromosome that behaves like a genetic switch controlling the presence or absence of male‐specific wing spots. This region includes the candidate gene optomotor‐blind (omb), which plays a critical role in patterning the Drosophila wing. The genetic basis of divergent wing display is more complex, with at least two loci on the X chromosome and two loci on autosomes contributing to its evolution. Introgressing the X‐linked region affecting wing spot development from D. gunungcola into D. elegans reduced pigmentation in the wing spots but did not affect the wing display, indicating that these are genetically separable traits. Consistent with this observation, broader sampling of wild D. gunungcola populations confirmed that the wing spot and wing display are evolving independently: some D. gunungcola males performed wing displays similar to D. elegans despite lacking wing spots. These data suggest that correlated selection pressures rather than physical linkage or pleiotropy are responsible for the coevolution of these morphological and behavioral traits. They also suggest that the change in morphology evolved prior to the change in behavior.

show abstract

“…This is possible because genital components usually have multiple functions (Wulff and Lehmann 2016; Genevcius and Schwertner 2017) and their development may be mediated by several genes and gene families (Dreyer and Shingleton 2019; Vincent et al. 2019). This multi‐factorial characteristic provides an avenue for distinct sources of variation available to selection, which in turn may facilitate the evolution of genital compartmentalization.…”

mentioning

confidence: 99%

Copulatory function and development shape modular architecture of genitalia differently in males and females

et al. 2020

View full text Add to dashboard Cite

Genitalia are multitasking structures whose development is mediated by numerous regulatory pathways. This multifactorial nature provides an avenue for multiple sources of selection. As a result, genitalia tend to evolve as modular systems comprising semi‐independent subsets of structures, yet the processes that give rise to those patterns are still poorly understood. Here, we ask what are the relative roles of development and function in shaping modular patterns of genitalia within populations and across species of stink‐bugs. We found that male genitalia are less integrated, more modular, and primarily shaped by functional demands. In contrast, females show higher integration, lower modularity, and a predominant role of developmental processes. Further, interactions among parts of each sex are more determinant to modularity than those between the sexes, and patterns of modularity are equivalent between and within species. Our results strongly indicate that genitalia have been subjected to sex‐specific selection, although male and female genitalia are homologous and functionally associated. Moreover, modular patterns are seemingly constant in the evolutionary history of stink‐bugs, suggesting a scenario of multivariate stabilizing selection within each sex. Our study demonstrates that interactions among genital parts of the same sex may be more fundamental to genital evolution than previously thought.

show abstract

An Atlas of Transcription Factors Expressed in Male Pupal Terminalia ofDrosophila melanogaster

Cited by 21 publications

References 100 publications

Unravelling the genetic basis for the rapid diversification of male genitalia betweenDrosophilaspecies

Unravelling the genetic basis for the rapid diversification of male genitalia betweenDrosophilaspecies

Co‐evolving wing spots and mating displays are genetically separable traits in Drosophila

Copulatory function and development shape modular architecture of genitalia differently in males and females

Contact Info

Product

Resources

About