An AutoAnalyzer method for estimating serum glyceride glycerol using a glycerokinase procedure

Abstract

SYNOPSISThe glyceride glycerol analysis depends, after saponification of triglycerides, on a linked enzymatic procedure using glycerokinase, pyruvate kinase, and lactate dehydrogenase: the final conversion of NADH to NAD+ is followed fluorimetrically. Twenty analyses can be performed per hour on the AutoAnalyzer; recoveries of added triglycerides ranged between 90 and 104 %. In a mixed male and female group the normal range for glyceride glycerol was 2 5 to 15 5 mg/100 ml (0-2-1-4 mmol/l) fasting, and 2-5 to 18-0 mg/100 ml (0.2-1-6 mmol/l) postprandially using fresh serum. There was a significant rise postprandially in older men.

Lipid methodology.

“…Automated methods have been described by Cramp (1970) and by Bell, Atkinson, and Baron (1970) using enzymatic procedures.…”

A Comparative Study of Manual Methods for Serum Triglyceride Estimation

“…An automated fluorimetric procedure has been described by Bell et al (1970) and manual kits have 9 been produced by Calbiochem (Calbiochem, 10 Wyndham Place, London W.l) and by Boehringer (Boehringer Corporation, London Ltd., Bilton House, Uxbridge Road, Ealing, London W.5). Both firms have also produced a semi-automated procedure employing the LKB 8600 Reaction Rate Analyser.…”