An automated assay for the clinical measurement of plasma renin activity by immuno-MALDI (iMALDI)

Popp, Robert; Malmström, David; Chambers, Andrew G.; Lin, David; Camenzind, Alexander G.; Gugten, J. Grace van der; Holmes, Daniel T.; Pugia, Michael J.; Jaremek, Marta; Cornett, Shannon; Suckau, Detlev; Borchers, Christoph H.

doi:10.1016/j.bbapap.2014.10.008

Cited by 24 publications

(28 citation statements)

References 36 publications

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“…Using the sensitivity of immunoaffinity capture combined with the specificity of mass spectrometry detection, he demonstrated results of plasma renin activity measurement, an established biomarker for primary aldosteronism [7].…”

Section: Proteomic Techniquesmentioning

confidence: 99%

Synopsis of the 4th Biannual Proteomic Forum: PhD student’s insight

Kiseleva

2015

Expert Review of Proteomics

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Section: Proteomic Techniquesmentioning

confidence: 99%

Synopsis of the 4th Biannual Proteomic Forum: PhD student’s insight

Kiseleva

2015

Expert Review of Proteomics

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“…Recently, iMALDI technology development has focused on automation (Fig. (a)), particularly for the measurement of plasma renin activity (PRA) as a screening method for primary aldosteronism (PA). Protein G Dynabeads with 2.8‐μm diameter were used for immuno‐enrichment.…”

Section: Mass Spectrometry‐based Assays With Antibody‐based Enrichmentmentioning

confidence: 99%

“…Protein G Dynabeads with 2.8‐μm diameter were used for immuno‐enrichment. The LLOD and linear range obtained met clinical requirements, and inter‐day CVs lower than 10% were obtained with an automated liquid handling system . The results of measuring patients' samples using iMALDI correlated well with those of clinically used LC–MS/MS assays and ELISA.…”

Section: Mass Spectrometry‐based Assays With Antibody‐based Enrichmentmentioning

confidence: 99%

“…Importantly, inexpensive benchtop MALDI‐TOF instruments such as the BioMerioux and the Bruker Biotyper have been FDA validated and cleared for microorganism identification, and many have already been installed in clinical and hospital laboratories. Because the high‐throughput iMALDI technology is compatible with benchtop MALDI instruments, this provides a perfect opportunity to introduce MS‐based diagnostics into a clinical setting.…”

Section: Mass Spectrometry‐based Assays With Antibody‐based Enrichmentmentioning

confidence: 99%

See 1 more Smart Citation

Current trends in quantitative proteomics – an update

Han

Pan

et al. 2017

J. Mass Spectrom.

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Proteins can provide insights into biological processes at the functional level, so they are very promising biomarker candidates. The quantification of proteins in biological samples has been routinely used for the diagnosis of diseases and monitoring the treatment. Although large-scale protein quantification in complex samples is still a challenging task, a great amount of effort has been made to advance the technologies that enable quantitative proteomics. Seven years ago, in 2009, we wrote an article about the current trends in quantitative proteomics. In writing this current paper, we realized that, today, we have an even wider selection of potential tools for quantitative proteomics. These tools include new derivatization reagents, novel sampling formats, new types of analyzers and scanning techniques, and recently developed software to assist in assay development and data analysis. In this review article, we will discuss these innovative methods, and their current and potential applications in proteomics. Copyright © 2017 John Wiley & Sons, Ltd.

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“…Two of the main advantages of MALDI-TOF-MS in comparison with classical LC-MS/MS are its high throughput and its cost effectiveness. This approach could also be used after using immunocapture methods to reduce the complexity of the samples[63,64].3.2. Electrospray ionization (ESI) triple quadrupole mass spectrometers: the MRM/SRM mode Multiple Reaction Monitoring (MRM) (or Selected Reaction Monitoring) methods are classically performed using a triple quadrupole (QqQ) mass spectrometer coupled with HighPerformance Liquid Chromatography (HPLC).…”

mentioning

confidence: 99%