An automated marine biomonitoring system for assessing water quality in real-time

Cunha, Sérgio; Gonçalves, Renata; Silva, Sérgio Rui; Correia, Ana D.

doi:10.1007/s10646-008-0216-y

Cited by 11 publications

(10 citation statements)

References 12 publications

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“…It demonstrated that the behavioral response of zebrafish to a noxious stimulus can be automated by MOBS. In the present experimental design we monitored four fish at the same time, but the present system could monitor up to 14 fish [11]. …”

Section: Discussionmentioning

confidence: 99%

“…The MOBS system is capable of measuring other behavioral activity patterns such as fish ventilation [11]. However, ventilation could not be measured in the present experimental design because the chambers were too large relative to the size of the fish.…”

Section: Discussionmentioning

confidence: 99%

“…A detailed description of this equipment is reported elsewhere [11]. Briefly, the system consists of a hardware device, a set of four experimental test chambers (cylindrical chambers, 6 cm in diameter, 10 cm long) and signal processing software running on an external computer.…”

Section: Methodsmentioning

confidence: 99%

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A Novel Behavioral Fish Model of Nociception for Testing Analgesics

et al. 2011

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Pain is a major symptom in many medical conditions, and often interferes significantly with a person's quality of life. Although a priority topic in medical research for many years, there are still few analgesic drugs approved for clinical use. One reason is the lack of appropriate animal models that faithfully represent relevant hallmarks associated with human pain. Here we propose zebrafish (Danio rerio) as a novel short-term behavioral model of nociception, and analyse its sensitivity and robustness. Firstly, we injected two different doses of acetic acid as the noxious stimulus. We studied individual locomotor responses of fish to a threshold level of nociception using two recording systems: a video tracking system and an electric biosensor (the MOBS system). We showed that an injection dose of 10% acetic acid resulted in a change in behavior that could be used to study nociception. Secondly, we validated our behavioral model by investigating the effect of the analgesic morphine. In time-course studies, first we looked at the dose-response relationship of morphine and then tested whether the effect of morphine could be modulated by naloxone, an opioid antagonist. Our results suggest that a change in behavioral responses of zebrafish to acetic acid is a reasonable model to test analgesics. The response scales with stimulus intensity, is attenuated by morphine, and the analgesic effect of morphine is blocked with naloxone. The change in behavior of zebrafish associated with the noxious stimulus can be monitored with an electric biosensor that measures changes in water impedance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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A Novel Behavioral Fish Model of Nociception for Testing Analgesics

et al. 2011

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“…Indeed, behavior has been used as an integral parameter of physiological activity and as a robust biological warning indicator of the quality of water supplies and effluents[6]–[7]. Fish ventilatory parameters that are known to be sensitive to toxins include ventilatory rate (opercular movement), depth or amplitude of ventilation, and coughing or gill purge rate [8].…”

Section: Introductionmentioning

confidence: 99%

Acute Toxicity, Respiratory Reaction, and Sensitivity of Three Cyprinid Fish Species Caused by Exposure to Four Heavy Metals

Wang

Liang

et al. 2013

PLoS ONE

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Using 3 cyprinid fish species zebra fish, rare minnow, and juvenile grass carp, we conducted assays of lethal reaction and ventilatory response to analyze sensitivity of the fish to 4 heavy metals. Our results showed that the 96 h LC50 of Hg2+ to zebra fish, juvenile grass carp, and rare minnow were 0.14 mg L−1, 0.23 mg L−1, and 0.10 mg L−1, respectively; of Cu2+0.17 mg L−1, 0.09 mg L−1, and 0.12 mg L−1 respectively; of Cd2+6.5 mg L−1, 18.47 mg L−1, 5.36 mg L−1, respectively; and of Zn2+44.48 mg L−1, 31.37 mg L−1, and 12.74 mg L−1, respectively. Under a 1-h exposure, the ventilatory response to the different heavy metals varied. Ventilatory frequency (Vf) and amplitude (Va) increased in zebra fish, juvenile grass carp, and rare minnows exposed to Hg2+ and Cu2+ (P<0.05), and the Vf and Va of the 3 species rose initially and then declined when exposed to Cd2+. Zn2+ had markedly different toxic effects than the other heavy metals, whose Vf and Va gradually decreased with increasing exposure concentration (P<0.05). The rare minnow was the most highly susceptible of the 3 fish species to the heavy metals, with threshold effect concentrations (TEC) of 0.019 mg L−1, 0.046 mg L−1, 2.142 mg L−1, and 0.633 mg L−1 for Hg2+, Cu2+, Cd2+, and Zn2+, respectively. Therefore, it is feasible to use ventilatory parameters as a biomarker for evaluating the pollution toxicity of metals and to recognize early warning signs by using rare minnows as a sensor.

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“…Adult fish (n=15) were treated intramuscularly (i.m.) with 33 mg/kg of 6-OHDA and the individual fish swimming responses recorded at various time-points after injections using an electrical biosensor, the MOBS system [23,24]. The dose, 33 mg/kg, was selected on the bases of literature data and from our pilot experiments.…”

Section: -Ohda Treatmentsmentioning

confidence: 99%

Green Fluorescent Protein Labeling of Dopaminergic Neurons in Zebrafish for the Study of Parkinson’s Diseases

Outeiro¹

2017

JMEN

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The loss of dopaminergic neurons in the substantia nigra is the pathological hallmark of Parkinson's disease (PD) associated with the characteristic motor deficits. Despite all the recent progress in the identification of genes and pathways associated with PD, we still lack a complete understanding of the molecular mechanisms underpinning the disease. Our goals were to develop a new transgenic zebra fish line where tyrosine hydroxylase (TH)-positive neurons were labeled with green fluorescent protein (EGFP) under the control of the zebra fish tyrosine hydroxylase (th1) promoter and to demonstrate the usefulness of the model for the study of the mechanisms underlying cell death in a toxin induced model of PD.A 2.5 kb gene fragment of the th1 promoter was isolated and cloned into a miniTol2vector followed by EGFP and microinjected to originate the Tg (-2.5th: EGFP) zebra fish line. Tg (-2.5th: EGFP) were visualized by fluorescence microscopy for EGFP and TH expression during development. The levels of EGFP mRNA were detected by RT-qPCR and protein expression was examined by western blot analysis. The model of neurotoxicity was generated by treating transgenic zebra fish with 6-hydroxydopamine (6-OHDA), followed by the analysis of cell death markers and behavior.EGFP expression was detected mainly in the posterior tuberculum of the ventral diencephalon (vDC). In the vDC, TH-labeled neurons of groups 1-were correctly labeled with EGFP. Further, we demonstrated that these neurons in larvae and adults are sensitive to 6-OHDA treatment, resulting in cell loss and behavioral deficits. In conclusion, this transgenic line constitutes a powerful tool to visualize and study dopaminergic neurons throughout the zebra fish life-cycle and enables high-throughput screening of molecules of interest for PD.

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An automated marine biomonitoring system for assessing water quality in real-time

Cited by 11 publications

References 12 publications

A Novel Behavioral Fish Model of Nociception for Testing Analgesics

A Novel Behavioral Fish Model of Nociception for Testing Analgesics

Acute Toxicity, Respiratory Reaction, and Sensitivity of Three Cyprinid Fish Species Caused by Exposure to Four Heavy Metals

Green Fluorescent Protein Labeling of Dopaminergic Neurons in Zebrafish for the Study of Parkinson’s Diseases

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