An automated method for the analysis of phenolic acids in plasma based on ion-pairing micro-extraction coupled on-line to gas chromatography/mass spectrometry with in-liner derivatisation

Peters, Sonja; Kaal, Erwin; Horsting, Iwan; Janssen, Hans‐Gerd

doi:10.1016/j.chroma.2011.10.055

Cited by 19 publications

(14 citation statements)

References 11 publications

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“…Trimethylsilyl (TMS) reagents, such as BSTFA/TMCS: N,O-Bis(trimethylsilyl)trifluoroacetamide: trimethylchlorosilane, MSTFA: N-Methyl-N-(trimethylsilyl)trifluoroacetamide or oximation/MSTFA, are the most widely used reagents for derivatizing phenolics and their metabolites [11][12][13][16][17][18][19]. Recently, alkylation of phenolics using tetrabutylammonium (TBA) salts was reported in an automated procedure in the GC injection port [20]. TMS derivatives have been successfully used especially in the analysis of metabolites in plasma and other biological matrices [8,11,12].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of microextraction by packed sorbent, liquid–liquid microextraction and derivatization pretreatment of diet‐derived phenolic acids in plasma by gas chromatography with triple quadrupole mass spectrometry

Bustamante

Cardenas

Baer

et al. 2017

J of Separation Science

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Miniaturized sample pretreatments for the analysis of phenolic metabolites in plasma, involving protein precipitation, enzymatic deconjugation, extraction procedures, and different derivatization reactions were systematically evaluated. The analyses were conducted by gas chromatography with mass spectrometry for the evaluation of 40 diet-derived phenolic compounds. Enzyme purification was necessary for the phenolic deconjugation before extraction. Trimethylsilanization reagent and two different tetrabutylammonium salts for derivatization reactions were compared. The optimum reaction conditions were 50 μL of trimethylsilanization reagent at 90°C for 30 min, while tetrabutylammonium salts were associated with loss of sensitivity due to rapid activation of the inert gas chromatograph liner. Phenolic acids extractions from plasma were optimized. Optimal microextraction by packed sorbent performance was achieved using an octadecylsilyl packed bed and better recoveries for less polar compounds, such as methoxylated derivatives, were observed. Despite the low recovery for many analytes, repeatability using an automated extraction procedure in the gas chromatograph inlet was 2.5%. Instead, using liquid-liquid microextraction, better recoveries (80-110%) for all analytes were observed at the expense of repeatability (3.8-18.4%). The phenolic compounds in gerbil plasma samples, collected before and 4 h after the administration of a calafate extract, were analyzed with the optimized methodology.

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Section: Introductionmentioning

confidence: 99%

Evaluation of microextraction by packed sorbent, liquid–liquid microextraction and derivatization pretreatment of diet‐derived phenolic acids in plasma by gas chromatography with triple quadrupole mass spectrometry

Bustamante

Cardenas

Baer

et al. 2017

J of Separation Science

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“…[95][96][97][98][99][100][101][102][103] In addition to UV, PDA, and MS, other detection methods for liquid chromatographic separations have been successfully applied over the years, including chemiluminescence reaction detectors, fluorescence detectors, and electrochemical detectors, especially for quantitation. [104][105][106][107][108][109][110][111] Gas chromatography/mass spectrometry (GC/MS) has been employed for the analysis of phenolic microbial fermentation products in biological samples in order to study the metabolic impact of flavonoid consumption [112][113][114] but has also been used for the direct analysis of the polyphenolic content of plant material. [115][116][117][118] The major drawback to GC analysis of polyphenols is that derivatization is generally required while with liquid chromatographic analyses derivatization is not required.…”

Section: Sample Analysismentioning

confidence: 99%

An Overview of Plant Phenolics Measurement

Yang¹,

Guido²

2018

Adv Food Technol Nutr Sci Open J

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Plant phenolics are widely distributed in nature and are well known for their remarkable antioxidative properties in biological systems such as plants and non-biological systems such as foods. Substantial work has been done to measure plant phenolics in different matrices; the four common steps involved in a measurement process are sampling, sample preservation, sample preparation, and analysis. This review addresses how sampling and sample preservation affect phenolic compounds levels and stability in different plant materials. Other important measurement factors including various sample extraction techniques and analysis methods are also reviewed in this paper.

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“…Essentially, any sorbent material and functionalization can be applied. For example, silica-based materials (C2, C8, C18), [114][115][116][117][118] with additional ion exchange functionality 119 or even as mixed-mode materials, 120 restricted access materials (RAM), HILIC, carbon, polystyrene-divinylbenzene copolymers (PS-DVB) or molecular imprinted polymers 113,121 have been utilized for MEPS.…”

Section: Monolithic Spin Column Extractionmentioning

confidence: 99%

“…The method has been implemented in several recent reports for quantication of pharmaceutical compounds from human biological samples (urine, plasma, oral uid and whole blood), including antipsychotic drugs, 119 cardiac drugs, 114 local anesthetics, 115,121 phenolic acid, 116 immunosuppressants, 117 opioids 120 and antidepressants. 118 Recent studies have also reported the successful extraction of trazodone from plasma with polymer nano-bers as the extraction sorbent.…”

Section: Monolithic Spin Column Extractionmentioning

confidence: 99%

Recent advances in sample preparation techniques to overcome difficulties encountered during quantitative analysis of small molecules from biofluids using LC-MS/MS

Bylda

Thiele

Kobold

et al. 2014

Analyst

224

106

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Liquid chromatography-mass spectrometry analysis of small molecules from biofluids requires sensitive and robust assays. Because of the very complex nature of many biological samples, efficient sample preparation protocols to remove unwanted components and to selectively extract the compounds of interest are an essential part of almost every bioanalytical workflow. This review describes the most common problems encountered during sample preparation, ways to optimize established sample preparation techniques and important recent developments to reduce or eliminate major interferents from biofluids.

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An automated method for the analysis of phenolic acids in plasma based on ion-pairing micro-extraction coupled on-line to gas chromatography/mass spectrometry with in-liner derivatisation

Cited by 19 publications

References 11 publications

Evaluation of microextraction by packed sorbent, liquid–liquid microextraction and derivatization pretreatment of diet‐derived phenolic acids in plasma by gas chromatography with triple quadrupole mass spectrometry

Evaluation of microextraction by packed sorbent, liquid–liquid microextraction and derivatization pretreatment of diet‐derived phenolic acids in plasma by gas chromatography with triple quadrupole mass spectrometry

An Overview of Plant Phenolics Measurement

Recent advances in sample preparation techniques to overcome difficulties encountered during quantitative analysis of small molecules from biofluids using LC-MS/MS

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