An automated packed Protein G micro-pipette tip assay for rapid quantification of polyclonal antibodies in ovine serum

Identifying analytics for high throughput bioprocess development studies

“…Chromatographic separations are often used as analytical tools for the detection and measurement of analytes of interest in process streams (Table S5). For example, Protein A analytical scale columns (Hober et al, 2007) or micro‐pipette assays (Chhatre et al, 2010) measure the total content of mAb in a sample. For the latter method, the main contribution to the total analytical time stems from the liquid handling of samples.…”

Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette Tips

“…Feed: Stocks of ovine serum containing polyclonal antibody must be maintained at −20 °C for long-term storage. To avoid the potentially deleterious impact of repeated freeze-thaw cycles of bulk material [7], it is recommended that small aliquots of around 1.5-2.0 mL volumes are frozen. Samples should be thawed fully to ambient temperature prior to experimentation.…”

“…To create an isotherm curve requires one to know the number of cycles needed to achieve equilibrium between solid and liquid phase at each concentration; hence isotherm data can be obtained from the end point of uptake curve data by material balance (see below) (see Notes 9-12). Uptake curves as a function of time can be plotted either in terms of the residual feed concentration, i.e., C = f(t), or in a normalized form by dividing individual data points by the initial feed antibody concentration, i.e., C/C 0 = f(t) [7]. The value of time at each data point accounts for the accumulated period covering both the aspirate and dispense steps.…”

Intelligent Automation Platform for Bioprocess Development

“…A large number of researches focus on the high throughput screening (HTS) which is an essential part of drug discovery and development (Gribbon & Andreas 2005;Mishra et al 2008). Many studies have contributed to microbial fermentation (Kostov et al 2001;Rios-Solis et al 2013 (Saitoh & Yoshimori 2008), precipitation (Knevelman et al 2010;Titchener-Hooker et al 2008) and chromatography (Chhatre et al 2010;Wiendahl et al 2008). …”

“…The tips enable the liquid handling system to investigate the whole chromatography processes. Several studies used micropipette chromatography tips to model the performance of column (Coffman et al 2008;Chhatre et al 2010). The third approach is to apply miniature columns.…”