An automated potentiometric assay for acid phosphatase

Ogończyk, Dominika; Głąb, Stanisław; Koncki, Robert

doi:10.1016/j.ab.2008.06.012

Cited by 12 publications

(3 citation statements)

References 9 publications

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“…Traditional assays for ACP are based on colorimetric determination of hydrolysis products of its substrates, which varied from p -nitrophenylphosphate and phosphotyrosine to 2,6-dichloro-4-acetylphenyl phosphate. − Although several assays based on potentiometric and chromatographic approaches were established to detect ACP level, − most efforts have been devoted to fluorometric assay because of its high sensitivity, convenience, and accessible instrument requirement. To date, only a few fluorescent assays for ACP have been proposed, and most of them mainly adopted organic dyes or fluorescent polymers as the fluorogenic indicators.…”

mentioning

confidence: 99%

Luminescent Aggregated Copper Nanoclusters Nanoswitch Controlled by Hydrophobic Interaction for Real-Time Monitoring of Acid Phosphatase Activity

et al. 2016

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A reversible luminescence nanoswitch through competitive hydrophobic interaction among copper nanoclusters, p-nitrophenol and α-cyclodextrin is established, and a reliable real-time luminescent assay for acid phosphatase (ACP) activity is developed on the basis of this luminescence nanoswitch. Stable and intensely luminescent copper nanoclusters (CuNCs) were synthesized via a green one-pot approach. The hydrophobic nature of CuNCs aggregate surface is identified, and further used to drive the adsorption of p-nitrophenol on the surface of CuNCs aggregate due to their hydrophobic interaction. This close contact switches off the luminescence of CuNCs aggregate through static quenching mechanism. However, the introduction of α-cyclodextrin switches on the luminescence since stronger host-guest interaction between α-cyclodextrin and p-nitrophenol causes the removal of p-nitrophenol from the surface of CuNCs. This nanoswitch in response to external stimulus p-nitrophenol or α-cyclodextrin can be run in a reversible way. Luminescence quenching by p-nitrophenol is further utilized to develop ACP assay using p-nitrophenyl phosphate ester as the substrate. Quantitative measurement of ACP level with a low detection limit of 1.3 U/L was achieved based on this specific detection strategy. This work reports a luminescence nanoswitch mediated by hydrophobic interaction, and provides a sensitive detection method for ACP level which is capable for practical detection in human serum and seminal plasma.

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confidence: 99%

Luminescent Aggregated Copper Nanoclusters Nanoswitch Controlled by Hydrophobic Interaction for Real-Time Monitoring of Acid Phosphatase Activity

et al. 2016

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“…The regression coefficient is 0.998, and the detection limit (LOD) for ACP was calculated to be 3.1 nU mL −1 . Compared with the previous reports for the ACP assay, [33][34][35][36] our method based on the CuInS 2 QDs obtained a lower detection limit and wider linear range.…”

Section: The Acp Assay Based On the Fluorescence Quenchingmentioning

confidence: 58%

Near-infrared fluorescence probe for the determination of acid phosphatase and imaging of prostate cancer cells

Lin

Liu

Zhang

et al. 2015

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In this paper, we developed a near-infrared mercaptopropionic acid (MPA)-capped CuInS2 quantum dot (QD) fluorescence probe for the detection of acid phosphatases (ACP), which is an important biomarker and indicator of prostate cancer. The fluorescence of CuInS2 QDs could be quenched by Cu(2+), and then the addition of adenosine-5'-triphosphate (ATP) could effectively turn on the quenched fluorescence due to the strong interaction between Cu(2+) and ATP. The ACP could catalyze the hydrolysis of ATP, which would disassemble the complex of Cu(2+)-ATP. Therefore, the recovered fluorescence could be quenched again by the addition of ACP. In our method, the limit of detection (LOD) is considerably low for ACP detection in solution. Using the CuInS2 QDs fluorescence probe, we successfully performed in vitro imaging of human prostate cancer cells.

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“…Through quantifying alcohols generated from ACP-catalyzed dephosphorylation of various orthophosphoric monoesters, several methods have been developed to probe ACP activity, including electrochemistry, , colorimetry, − fluorimetry, − potentiometric immunoassay, and chromatography . Among these methods, colorimetry, featuring simplicity, cost-effectiveness, rapidity, and visibility, has attracted extensive attention.…”

Section: Introductionmentioning

confidence: 99%

Engineering Two-Dimensional Pd Nanoplates with Exposed Highly Active {100} Facets Toward Colorimetric Acid Phosphatase Detection

Chen

et al. 2019

ACS Appl. Mater. Interfaces

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Enzyme-like activity and efficiency of nanomaterials are strongly controlled by their size, composition, and structure, and hence the structural parameters need to be optimized. Here, we report that two-dimensional Pd nanoplates enclosed by {100}-facets [{100}PdSP@rGO] exhibit substantially enhanced intrinsic oxidase-like activities relative to the {111}-facets ones and Pd nanocubes in catalyzing the chromogenic reaction of 3,3′,5,5′-tetramethylbenzidine. By taking ascorbic acid 2-phosphate as the substrate, which transforms to ascorbic acid in the presence of acid phosphatase (ACP), the {100}PdSP@rGO could be used as an efficient nanozyme for colorimetric ACP detection without resorting to destructive H2O2. A good linear relationship from 0.01 to 6.0 mU/mL with a detection limit of 8.3 μU/mL is obtained, which is better than most previously reported ACP assays. Importantly, the excellent assay performance could be successfully applied to ACP determination in serum samples with high accuracy. This study demonstrates that the enzyme-like activity of nanomaterials could be finely tuned simultaneously by controlling their exposed crystal facets and high specific surface area.

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An automated potentiometric assay for acid phosphatase

Cited by 12 publications

References 9 publications

Luminescent Aggregated Copper Nanoclusters Nanoswitch Controlled by Hydrophobic Interaction for Real-Time Monitoring of Acid Phosphatase Activity

Luminescent Aggregated Copper Nanoclusters Nanoswitch Controlled by Hydrophobic Interaction for Real-Time Monitoring of Acid Phosphatase Activity

Near-infrared fluorescence probe for the determination of acid phosphatase and imaging of prostate cancer cells

Engineering Two-Dimensional Pd Nanoplates with Exposed Highly Active {100} Facets Toward Colorimetric Acid Phosphatase Detection

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