Near-infrared fluorescence probe for the determination of acid phosphatase and imaging of prostate cancer cells

Abstract

In this paper, we developed a near-infrared mercaptopropionic acid (MPA)-capped CuInS2 quantum dot (QD) fluorescence probe for the detection of acid phosphatases (ACP), which is an important biomarker and indicator of prostate cancer. The fluorescence of CuInS2 QDs could be quenched by Cu(2+), and then the addition of adenosine-5'-triphosphate (ATP) could effectively turn on the quenched fluorescence due to the strong interaction between Cu(2+) and ATP. The ACP could catalyze the hydrolysis of ATP, which would disassemble the complex of Cu(2+)-ATP. Therefore, the recovered fluorescence could be quenched again by the addition of ACP. In our method, the limit of detection (LOD) is considerably low for ACP detection in solution. Using the CuInS2 QDs fluorescence probe, we successfully performed in vitro imaging of human prostate cancer cells.

Near-infrared quantum dots based fluorescent assay of Cu2+ and in vitro cellular and in vivo imaging

“…To address this issue, the new generation near-infrared QDs (NIR QDs) have been introduced and gradually attracted the interest of researchers due to its multifaceted advantages, including minimum photo-damage, deep penetration into biological tissues, lower fluorescence background, and weak scattering of emission light [32][33][34][35][36]. However, only a limited number of NIR QDs, such as Ag 2 Se [34], Ag 2 S [35], CuInS [37], Zn x Hg (1-x) Se [38], PbS [39], InP QDs [40] etc., has been reported and successfully synthesized and demonstrated as promising canScheme 1. The near infrared QDs based detection of Cu 2+ and monitoring the change of Cu 2+ concentration in vitro and in vivo through fluorescent imaging.…”

Smartphone colorimetric assay of acid phosphatase based on a controlled iodine-mediated etching of gold nanorods

“…In recent decades, a variety of strategies using colorimetry [2,[7][8][9][10][11][12][13][14], fluorescence [15][16][17][18][19], surface-enhanced Raman scattering [20], and electrochemistry [21,22] have been established for detecting ACP. Compared with other traditional detection methods, colorimetric assays have advantages such as fast response, simple operation, real-time visualization, and low cost.…”

Fluorescence imaging of hepatocellular carcinoma with a specific probe of COX-2

“…[8][9][10] Because of their high-accuracy and high-selectivity, and especially, non-radioactivity, uorescent probes may offer a more useful strategy for the diagnosis of tumors. 11,12 Cyclooxygenase-2 (COX-2) is an induced type of cyclooxygenase. For the most part, this type of enzyme is not expressed in normal tissue, but when stimulated by a tumor or infection, the expression of COX-2 will obviously increase.…”