An azophenolic colorimetric reagent for use in enzyme-linked immunosorbent assays

The biotin-streptavidin (BA)-ELISA system was optimized for the detection of three potyviruses infecting cucurbits, the watermelon strain of papaya ringspot virus (PRSV-W), zucchini yellow mosaic virus (ZYMV) and watermelon mosaic virus 2. BA-ELISA was four to eight times more sensitive than double antibody sandwich ELISA and detected all three viruses in the nanogram range. BA-ELSA was virus-specific but did not differentiate between different pathotypes of ZYMV. The use of egg-white avidin-enzyme conjugate or the simultaneous incubation of biotinylated antibody and streptavidin-enzyme conjugate decreased the sensitivity of BA-ELISA. The concentrations of PRSV-W and ZYMV in field-infected cucurbit specimens or in resistant breeding lines were estimated from calibration curves derived from a dilution series of purified virus in extracts of uninfected plants on each test plate. Thus it was possible to determine semi-quantitatively the degree and uniformity of resistance in plants from these lines and to differentiate resistant from tolerant selections.

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An azophenolic colorimetric reagent for use in enzyme-linked immunosorbent assays

Cited by 2 publications

References 9 publications

Fluorimetric-flow injection determination of theophylline based on its inhibitory effect on immobilized alkaline phosphatase

Fluorimetric-flow injection determination of theophylline based on its inhibitory effect on immobilized alkaline phosphatase

A sensitive semi-quantitative biotin-streptavidin ELISA for the detection of potyviruses infecting cucurbits

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